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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 24 (1986), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Twelve monoclonal antibodies specific for Sendai virus were prepared by fusing immune LOU rat spleen cells with Y3 or FO myeloma cells. Six antibodies bound the viral glycoprotein HN, and six the viral protein F. Among the six HN-specific monoclonal antibodies, five reacted with the very same epitope and inhibited viral haemagglutination. Two antibodies against the F protein recognized the same epitope, but all the others reacted with different epitopes. All monoclonals were characterized with regard to specificity, biological function, epitope recognition, isotypes, and pI.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 24 (1986), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The monoclonal antibodies A and B specific for the HN molecule of Sendai virus were used to induce polyclonal and monoclonal anti-idiotypic antibodies. No response was observed in allogeneic Lewis rats, low responses in syngeneic LOU rats, and high responses in allogeneic BN rats and xenogeneic Balb/c mice. The monoclonals A and B share a similar or identical idiotype. since polyclonal anti-idiotypic antisera to antibody A cross-reacted completely with antibody B and vice versa. The same was found with the three monoclonal anti-idiotypes 1, 2, and 3elicited in a BN rat or in Balb/c mice. None of the polyclonal or monoclonal anti-idiotypes reacted with other monoclonal antibodies specific for Sendai virus, even when these recognized the same epitope as antibodies A and B. The monoclonal anti-idiotypic antibodies could be used to induce anti-Sendai antibodies in BN rats.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 21 (1985), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Polyclonal anti-idiotypic antibodies against monoclonal antibodies with specificity for RT1-encoded antigens were induced in syngeneic, allogeneic, and xenogeneic hosts. The immune response to the idiotypic determinants on monoclonal antibodies was T-cell-dependent. The anti-idiotypic antibodies, independent of whether they were induced in syngeneic, allogeneic, or xenogeneic (after proper absorption) hosts, showed an exquisite specificity for the monoclonal antibody used for the induction. No cross-reactivity with other monoclonal antibodies could be observed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 21 (1985), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Sixteen monoclonal anti-idiotypic antibodies were prepared against RTI antigen-specific monoclonal antibodies by means of an inhibition assay. Three (110-3, 112-89, and 133-4) were produced against antibody 3-8C2 (19), three (178-60, 181-14, and 184-38) against antibody 11/23/5, and three (144-3, 144-33, and 146-31) against antibody 1-5A4. One monoclonal antiidiotypic antibody (21–39) was directed against antibody 1-8D2 and six (CE1-3, CE2-1, CE7-2, CE8-46, CE9-35, and CE 10-2) against antibody 3-12A1. The anti-idiotypic antibodies were all highly specific for the respective monoclonal antibody used for the induction of the antiidiotype and did not cross-react with other monoclonal antibodies.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 9 (1979), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Rat T lymphocytes were activated in MLC (mixed leucocyte culture) against allogeneic stimulator cells. A few percent of such T blasts could be shown to carry IgM on their surface when a mixture of T and B lymphocytes was used as responder cells. The immunoglobulin was absent when purified T lymphocytes served as responders, A large proportion of MLC T lymphoblasts could be shown to carry stimulator alloantigens on their surface. These antigens were demonstrated (a) by indirect immunofluorescence using anti-stimulator alloantibody and FITC (fluorescein isothiocyanate Isomer I)-labelled F(ab)2 fragments of rabbit anti-rat Ig; (b) by autoradiography after internal labelling of stimulator cells. which showed the transfer of label from stimulator to responder cells. For biochemical analysis T blasts were restimulated with internally labelled allogeneic or syngeneic stimulator cells, and bound radiolabelled stimulator alloantigens were analysed on SDS-PAGE (sodium lauryl sulphate polyacrylamide gel electrophoresis). The results suggest that intact SD antigens with a molecular weight of 45,000 daltons and LD determinants with a molecular weight of 34,000 daltons derived from stimulator cells are found on MLC T blasts. No evidence of binding of the 27,000-dalton la chain to T blasts was found. Possible arrangements of these molecules on the responder blasts is discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 23 (1986), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Polyclonal syngeneic, allogeneic. and xenogeneic and monoclonal syngeneic anti-anii-idiotypic antibodies have been produced against previously described monoclonal anti-idiolypic anti-bodies with specificity for monoclonal RTI alloantigen-specific antibodies. The anti-anti-idiolypes could again be shown to be highly specific for the monoclonal anti-idiotype used for the induction of the anti-anti-idiotypie antibodies and lo carry the same, or a very similar, idiotype as the original monoclonal idiotypic antibody used lo induce the monoclonal anti-idiotypic. Among the 30syngeneic and allogeneic and the live xenogeneic polyclonal anti-anti-idiotypic antisera and the three monoclonal anti-anti-idiotypes. only one polyclonal antiserum showed binding capacity to the corresponding RT1-eneoded antigenic determinants on spleen cells. All the oilier antibodies were idiotypic but not antigen binding.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 21 (1985), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Sixteen monoclonal antibodies against antigens coded for by the RT1 complex of the rat have been produced. Fourteen arc specific for the a haplotype: six recognize class II and eight class I antigens. Two are specific for the I haplotype, one reacting with class I and the other with class II antigens. By means of these monoclonal antibodies four independent clusters of antigens for class I antigens of the a haplotype and three for class II antigens could be defined. The three antigenic sites of class II antigens reside on the same heterodimer. The monoclonals described here are characterized with regard to Ig class and subclass, pI, and complement-activating capacity.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 35 (1995), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: This experiment investigated the effect of pre-germination moist chilling of seeds (for 3 weeks at 2^C) of 10 common arable weeds on the subsequent growth and flowering of the plants. In only three of the species (Alopecurus myosuroides Huds., Veronica persica Poir. and Trifolium dubium Sibth.) was flowering markedly increased by the seed chilling treatments. At 3 months, the mean number of flowers and seed heads per plant on the treated plants was greater than that on the controls by factors of 13.9, 2.02 and 1.74 respectively. Shoot lengths of the seed-chilled plants were also significantly greater in all three species, though dry weights did not differ significantly from those of the controls. Thus, the plants showed morphological changes associated with reproduction but no significant alteration in total dry weight. The ecological implications of the data are discussed in relation to possible effects of climatic change on species which require seed vernalization.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 20 (1980), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The seeds of thirty-two species of common East African weeds were set to germinate in the light and in the dark. Eighteen species with more than 40% germination in the light were also germinated in leaf shade and in neutural shade of equal intensity. Germination of fifteen of these was inhibited by darkness, and of sixteen by leaf shade, with complete inhibition in six species. A high degree of dormancy was found in fourteen species. Only one species in this group responded to alternate wetting and drying of the seeds. In eight species dormancy was less in older seeds.The ecological implications of the results are briefly discussed, and comparisons made with temperate weeds.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1939
    Keywords: Herbivory ; Wound-induced defence ; Cabbage ; Pieris brassicae ; Spodoptera littoralis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper presents tests of a model of wound-induced defence in herbaceous plants. Many studies have reported both chemical changes in leaves and changes in the behaviour and/or physiology of herbivores as a result of wounding leaves. These studies and others have led to the development of various models to explain wound-induced effects both in terms of plant response and herbivore behaviour. The model under test was proposed by Edwards and Wratten (1987) and predicts that wounding a plant will cause herbivores (1) to take more meals of a smaller size and/or consume less foliage overall (2) grow more slowly and (3) be more mobile. These predictions were tested in cabbage Brassica oleracea L. var. Capitata cv. Pixie with Pieris brassicae L. (Lepidoptera: Pieridae) as a herbivore specialising on cabbage, and Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae) as a generalist herbivore. Both insects showed some reduction in consumption of leaves from upper parts of the plant, but no change in meal size. There were no effects on the growth or mobility of either species as a result of wounding foliage. These results are discussed in relation to the predictions of the model.
    Type of Medium: Electronic Resource
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