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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 206 (1998), S. 63-67 
    ISSN: 1431-4630
    Keywords: Key words Anaerobiosis ; Injury ; Bacillus stearothermophilus ; Process optimization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The effect of recovering Bacillus stearothermophilus spores under anaerobic conditions on their apparent thermal resistance was studied. Spores were suspended in bidistilled water as a reference medium, heated at 115, 117, 119, 121, 123 and 125°C and recovered under aerobic and anaerobic conditions. D values (decimal reduction time) obtained following recovery under anaerobic conditions were lower than those obtained under aerobic conditions. Reductions of between 31 and 48% were found for all the temperatures studied. When spores were suspended in mushroom extract and recovered under anaerobic conditions the apparent heat resistance was much lower than that obtained under aerobic conditions (D 121°C was 4.3 min and 1.7 min, under aerobic and anaerobic conditions, respectively). Heating the spores in mushroom extract and recovering the spores under anaerobic conditions produced an additive effect, decreasing the apparent heat resistance of the B. stearothermophilus spores.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 206 (1998), S. 58-62 
    ISSN: 1431-4630
    Keywords: Key words Bacillus stearothermophilus ; Predictive ; microbiology ; Thermal resistance ; Sodium chloride ; Environmental factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The effect of different concentrations of sodium chloride (NaCl) in the recovery medium on the apparent thermal resistance of Bacillus stearothermophilus spores (strain ATCC 12980) was studied. Spores were heated at different temperatures (115–125°C) and subcultured in medium with different NaCl concentrations [1, 2, 3 and 3.5% (w/v)]. The results indicate that increasing NaCl levels decreases the apparent heat resistance of the spores, over a temperature range of 115–125°C. No significant difference was found between 3 and 3.5% (w/v) NaCl although both reduced B. stearothermophilus heat resistance considerably. A second-order polynomial equation provided a good description of the spores’ D value, as influenced by the temperature and NaCl concentration. Predictions from the model compared well with published data, and therefore the model is suitable for predicting the effect of NaCl concentration on thermal inactivation of B. stearothermophilus.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 207 (1998), S. 322-327 
    ISSN: 1431-4630
    Keywords: Key words Mandarin ; Enzymatic peeling ; Peeling process ; Fresh fruit ; Modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A process for enzymatic peeling of mandarin was optimised. Enzymatic peeling was completed by means of infusion under vacuum of a commercial preparation of pectolases and cellulases (Rohament PC), followed by a period of incubation with the prepared enzymatic solution, elimination of the skin and separation of the segments. Distinct designs of the cuts of the flavedo were studied, by analysing characteristics of enzymatic saturation at distinct pressures and times of vacuum. Based on the data obtained, a mathematical model was developed which allowed us to predict the infusion of enzyme reached as a function of pressure and time of vacuum. The best peeling results were achieved in those combinations of pressure and time of vacuum that allowed a saturation of approximately 90% in less than 10 min. The design of an ideal cut (a cut in the equatorial zone, the removal of part of the peel of the calycinal and peduncular zones and two longitudinal cuts), two suitable vacuum conditions (defined using the mathematical model; 400 mm Hg and 500 mm Hg during 5 min), the effect of distinct concentrations of Rohament PC and incubation times on the main attributes of peeling quality (ease of separation of the remains of the residual peel, ease of segment separation, percentage of viable segments and percentage of albedo not attacked by the enzyme) were studied. The greatest quantity of viable segments and the best quality of enzymatic peeling were achieved by using an enzymatic concentration of 5 g/l of Rohament PC and an incubation time of 20 min. Under these conditions, 90% and 85% of viable segments were obtained, less than 10% of the albedo was not attacked, an easy separation of the remains of the residual peel and easy separation of the segments were achieved.
    Type of Medium: Electronic Resource
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