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  • 1
    Electronic Resource
    Electronic Resource
    Annexin gene structures and molecular evolutionary genetics (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 508-515 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Chromosomal maps; exon-intron splicing; gene structures; gene duplication dates; homologous recombination; molecular evolution; multigene family; phylogenetic analysis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Annexins provide an exemplary model for studying the pattern and process of molecular evolution in multigene families. Their related gene structures, broad dispersal in eukaryotic genomes and abundant coding sequences permit a phylogenetic reconstruction of their genetic history. The emerging picture is one of prolific expansion by gene duplication to more than 27 paralogous subfamilies that have undergone steady sequence divergence, speciation and differential selection. Homologous recombination via the common tetrad of internal repeats has, nevertheless, strictly preserved this core structure for over 1200 million years, implying a basic functional role. The existence of multiple annexins with unique 5′ coding and regulatory regions has facilitated their adaptation to the varying ontogenetic and cell-specific needs of diverse organisms. Computational and cladistic sequence analyses have permitted the determination of original gene duplication dates and mutation rates for the ten known vertebrate annexins. Molecular genetic and evolutionary studies of annexins can help to define their structure-function relationships, elucidate their individual physiological roles and ultimately link them to hereditary phenotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050064
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  • 2
    Electronic Resource
    Electronic Resource
    A BC200-derived element and Z-DNA as structural markers in annexin I genes: Relevance to Alu evolution and annexin tetrad formation (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 979-985 
    Details
    ISSN: 1432-1432
    Keywords: Alu subfamily ; Annexins ; BC200 gene ; Calcium-binding protein ; Gene structure ; Genomic DNA ; Molecular evolution ; Molecular sequence data ; Structural elements ; Z-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified two types of structural elements in genomic DNA for annexin I that provide physical evidence of genetic events leading to conserved changes in gene structure. The sequence upstream of the transcribed region in human annexin I contained a rare, Alu-like repetitive element with flanking direct repeats, probably derived from the active BC200 gene via germline retroposition. Nucleotide substitutions in this BC200 insert relative to the 7SL gene and its absence in rodent annexins I identified it as a recent primate pseudogene. Phylogenetic analysis showed that the BC200 gene represents a new clade of primate Alu evolution that branched near the time of appearance of the progenitor to the free left Alu monomer, FLAM-C. Separate analysis identified a Z-DNA motif in pigeon annexin I intron 7 that may represent the vestigial recombination site involved in primordial assembly of the annexin tetrad. These distinct structural features in annexin I genes provide insight into the evolution of Alu repeats and the mechanism of annexin tetrad formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173179
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  • 3
    Electronic Resource
    Electronic Resource
    The gene encoding human annexin V has a TATA-less promoter with a high G + C content
    Amsterdam : Elsevier
    Gene 149 (1994), S. 253-260 
    Details
    ISSN: 0378-1119
    Keywords: Calcium-binding protein ; cis-regulatory elements ; gene structure ; genomic DNA sequence ; phospholipase A2 inhibitor ; protein kinase C inhibitor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(94)90157-0
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  • 4
    Electronic Resource
    Electronic Resource
    Structure of the gene encoding anchorin CII (chick annexin V)
    Amsterdam : Elsevier
    Gene 141 (1994), S. 179-186 
    Details
    ISSN: 0378-1119
    Keywords: Genomic DNA ; calcium-binding protein ; cis-regulatory elements ; collagen-binding protein ; lipocortin V ; phospholipid-binding protein
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(94)90568-1
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  • 5
    Electronic Resource
    Electronic Resource
    Distribution and remobilization of symbiotically fixed nitrogen in soybean (Glycine max) (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 65 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Partitioning of nitrogen by soybeans (Glycine max L. Merr. cv. Hodgson) grown in natural conditions was studied by successive exposures of root systems to 15N2 and periodical measurements of 15N distribution. Nitrogen derived from the atmosphere was mainly found in the aerial parts of the plants, and the stage of development exerted a strong influence on the initial 15N distribution (measured one week after incorporation). Until day 69 after sowing, leaf blades contained 47 to 57% of the fixed N. After that, reproductive structures attracted increasing proportions, 10 to 60% between days 69 and 92. Around day 82, stems and petioles stored up to 30% of the newly fixed N. During pod development and pod filling and until maturity, fixed N was remobilized from vegetative tissues and pod walls to seeds. These transfers first concerned the newly incorporated N, but at maturity 80 to 90% of the total was recovered in the seeds. The high mobility of N originating from the atmosphere as compared to that coming from the soil (vegetative tissues exported only 50% of their total N) seems to indicate that fixed N was at least partially integrated in a special pool. This was certainly the case at the later stage of N2 fixation, when a large portion of fixed N accumulated in the stems and petioles, probably in the form of storage compounds such as ureides for later transfer to the developing seeds. Further research is needed in order to investigate the nature and role of this pool in the nitrogen nutrition of soybeans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb02396.x
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic complementation of rhizobial nod mutants with Frankia DNA: artifact or reality? (1998)
    Springer
    Molecular genetics and genomics 260 (1998), S. 115-119 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsFrankia ; Nodulation ; Complementation ; Tn5 mutant ; Rhizobiaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two divergent reports have been published on the genetic complementation of rhizobial nod mutants using Frankia DNA. In 1991 putative Frankia cosmid library clones were reported to restore normal nodulation properties to Rhizobium leguminosarum biovar viciaenodD::Tn5, but no supporting sequence data were published. In 1992 a second group reported a failure to find any evidence of functional complementation of various rhizobial nod mutants by Frankia DNA (nodA, nodB and nodC). Complementation tests of nine Nod− R. leguminosarum bv. viciae or Sinorhizobium meliloti Tn5 mutants (nodA − , nodB − , nodC − , nodD − , nodF  − , nodL − , nodH − ) were thus performed using a Frankia gene library in pLAFR3 to clarify this situation. Rhizobial transconjugants obtained by tri-parental matings were screened for restoration of the nodulation phenotype on their host plants, Vicia sativa subsp. nigra or Medicago sativa. Nodulation was observed on plants inoculated with transconjugants of the R. leguminosarum bv. viciaenodC::Tn5 mutant. The Nod+ rhizobial transconjugants were isolated and analysed. The Nod+ phenotype of these transconjugants was found to be due to Tn5 excision/transposition. No functional complementation was found with any of the mutants used, suggesting that rhizobial complementation of nod mutants with Frankia DNA is unlikely to occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050877
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  • 7
    Electronic Resource
    Electronic Resource
    Diffusion du15N2 dans le sol pendant la mesure de fixation biologique de l'azote (1983)
    Springer
    Plant and soil 73 (1983), S. 431-434 
    Details
    ISSN: 1573-5036
    Keywords: Nitrogen fixation ; 15N2 diffusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary The kinetic of15N2 diffusion has been measured in a system similar to that for the estimation of N2 fixation in plant microorganism associations cultivated in soil. The15N2 enrichment of the soil atmosphere reached an homogenous value one hour after injection of15N2 and is identical to that obtained by calculation, indicating that no adsorption occurs in the soil particles.
    Notes: Résumé La cinétique de diffusion du15N2 est mesurée sur un système identique à ceux pouvant être utilisés pour la mesure de fixation de l'azote chez les associations plantes-microorganismes cultivées sur sol. L'enrichissement homogène de l'atmosphère du sol est obtenu une heure environ après l'injection de15N2 et correspond à l'enrichissement calculé, ce qui indique qu'aucune adsorption n'a lieu dans les particules du sol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184321
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  • 8
    Electronic Resource
    Electronic Resource
    Distribution and utilization of assimilated carbon in red clover during the first year of vegetation (1987)
    Springer
    Plant and soil 97 (1987), S. 131-143 
    Details
    ISSN: 1573-5036
    Keywords: Assimilates distribution14CO2 labelling ; C remobilization ; N2 fixation ; Respiration costs ; Trifolium pratense L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The pattern of distribution of14C labelled assimilates and translocation with time was measured in red clover during one reproductive cycle. Measurements were made on whole plants grown outdoors in pots by exposing the aerial parts to14CO2 during one photoperiod. Simultaneously, root respiration and N2 fixation were recorded. At the beginning of the vegetative period, 2/3 of the assimilates remained in the leaves (basal leaves), and 1/3 were directed to the root system. Then the development of branches required as much as 40% of the C and the root allocation decreased. Reproductive structures diverted 17% of the current photosynthates. Nitrogen fixation was optimal during the maximum extension of the basal leaves and decreased during the development of branches. During this period, C allocation to the nodulated roots was high with an estimated amount of 3.2 mg of C per mg of N fixed. With time, translocation occured within the foliage, from basal leaves to the leaves of the branches and to the new basal leaves developed after senescence of the branches. Remobilization to the reproductive structures remained minimal indicating that flower and seed growth was supported by current photosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02149831
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