ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Experimental determination of the instrumental transparency function of texture goniometers (2000)

Moras, K. ; Fischer, A. H. ; Klein, H. ; [et al.]

Copenhagen : International Union of Crystallography (IUCr)

Applied crystallography online 33 (2000), S. 1162-1174

add to mindlist on the mindlist

Details

ISSN: 1600-5767

Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001

Topics: Geosciences , Physics

Notes: The instrumental transparency functions of five commercially available texture goniometers were measured experimentally with six monocrystalline samples cut in different orientations from a large highly perfect silicon crystal with a rocking curve of less than 0.01°. Transparency functions were measured in steps of 0.02 to 0.2° in the pole-figure angles α, β. The window size Δα depends on the Bragg angle θ in the form 1/sinθ; the window size Δω is constant for each goniometer. The dominant instrumental parameter determining the long axis Δα of the pole-figure window is the axial width of the detector entrance slit. This parameter is smallest for area detectors (smaller by more than an order of magnitude compared with conventional scintillation detectors as well as one-dimensional position-sensitive detectors). The main features of the pole-figure window and their dependence on the instrumental parameters can be deduced fairly well from a simple geometrical model. The particular shapes of the transparency functions of the studied goniometers are markedly different. Particularly, they are not very well represented by Gauss functions. The two-dimensional transparency function can be fairly well characterized by its α and β profiles. The normalized profiles are virtually independent of the goniometer angles 2θ and α. The increasing size of the pole-figure window with decreasing θ puts a lower limit on the Bragg angle below which pole-figure measurement ceases to be meaningful.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1107/S0021889800007251

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes (1988)

Shipman, P. M. ; Sabath, D. E. ; Fischer, A. H. ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 38 (1988), S. 189-198

add to mindlist on the mindlist

Details

ISSN: 0730-2312

Keywords: cyclin ; proliferating cell nuclear antigen ; cloned T lymphocytes ; interleukin 2 ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Expression of cyclin, a non-histone nuclear protein, during recombinant interleukin 2 (rIL2)-driven cell-cycle progression of cloned T lymphocytes has been assessed. We found that expression of cyclin protein, as detected by immunofluorescence, is tightly associated with proliferation, and not merely S-phase, of L2 cells stimulated with rIL2. Cyclin immunofluorescence was detected in all cell-cycle phases (G1/S/G2/M, as detected by flow cytometry) of proliferating L2 cells. Accumulation of cyclin mRNA levels was induced as early as 1 h after stimulation, was maximal at 25-49 h, and remained elevated throughout stimulation, as detected by Northern blot analysis. A cDNA-encoding murine cyclin was cloned from a cDNA library prepared from IL2-stimulated cloned T cells. The sequence of the 5′ end of the murine cyclin cDNA was determined and found to be 88% and 82% similar to the sequences of cDNA clones encoding rat and human cyclin, respectively. The present studies demonstrate that cyclin protein and mRNA accumulation are highly regulated during IL2-induced proliferation of a cloned T cell. These data provide a framework for addressing the molecular mechanisms regulating cyclin gene expression during cellular proliferation.

Additional Material: 4 Ill.