ISSN:
1420-908X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Effects of aspirin (200 μg/ml), hydrocortisone (10 μg/ml), sodium aurothiomalate (100 μg/ml), and indomethacin (10 μg/ml) on recovery of cartilage from interleukin 1 or catabolin-induced degradation were examined in this initialin vitro study. The experimental protocol involved a “degradative phase” of eight days during which cartilage plugs were incubated in the presence or absence of spent human rheumatoid synovial culture media. A “recovery” period of six days followed during which the effects of the aforementioned drugs on treated cartilage were analyzed. Incorporation of [35S]sulfate and [3H]proline precursors, and total contents of hydroxyproline and glycosaminoglycan in cartilage were determined two, four, and six days after insult. Aspirin treatment caused a rise in total proteoglycan content over degraded controls (p〈0.002), however, this increase was not associated with increased [35S]sulfate incorporation into glycosaminoglycans. Hydrocortisone resulted in a delayed rise in proteoglycan content concommitant with increased [35S]sulfate uptake, whereas sodium aurothiomalate treatment was without effect on proteoglycans. Indomethacin treatment was associated with an increased release of newly synthesized macromolecules by cartilage into the media (p〈0.01). These results suggest that common anti-inflammatory drugs may exhibit distinctly different effects on thein vitro synthesis and retention of proteoglycans by cartilage explants previously exposed to a degradative phase. Further work is necessary to assess the influence of drug concentration in this experimental system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01974935
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