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  • 1
    Electronic Resource
    Electronic Resource
    Scientific Workers and War (1936)
    [s.l.] : Nature Publishing Group
    Nature 138 (1936), S. 80-80 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE article on “War, Science and Citizenship” in NATURE of May 9, and the letter on “Scientific Workers and War” published in the following week have expressed views no doubt widely held among readers of NATURE. Neither, however, gives any adequate suggestion as to how those scientific workers ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/138080a0
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosomal Variation in Lotus alpinus (Fabaceae) (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant species biology 4 (1989), S. 0 
    Details
    ISSN: 1442-1984
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract An accession of Lotus alpinus Schleich. (2n=2x=12) from Turkey in which B chromosomes have been found was studied morphologically and karyologically. Chromosome numbers were observed in 519 cells from nine plants which all exhibited mixoploidy (2n=11, 12, 12 + 1B, 12 + 2B and over 20). Keel tip color, stem pubescence, and inflorescence size differed from a collection of this species from Switzerland. While the percentage of total lengths of the chromosome complements and the relative chromosome lengths in the two accessions were very similar, the total complement lengths differed considerably (23.14μm Turkey vs. 29.46μm Switzerland). This karyological difference is not considered to be the result of the presence of B chromosomes, but probably the result of hybridization between different genotypes. Aborted seed pods were observed which lent credibility to this hypothesis. Plants of this accession may have arisen as a result of hybridization between Lotus corniculatus and/or L. alpinus as both diploid and tetraploid cytotypes are reported in the Turkey collection for these species. The data would lend support for their hybrid origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1442-1984.1989.tb00053.x
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  • 3
    Electronic Resource
    Electronic Resource
    An investigation into the mechanism for reduced seed yield inLotus corniculatus (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 157-160 
    Details
    ISSN: 1432-2242
    Keywords: Lotus corniculatus ; Birdsfoot trefoil ; Seed set ; Cross-pollination ; Self-pollination ; Self-discrimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A study of seed position in the pod ofLotus corniculatus L. cv. ‘Mirabel’ (Fabaceae) suggested that reduced seed set after self-pollination is not due to an inability of the self-pollen tube to reach the end of the ovary. As in other cultivars, it has been demonstrated that cultivar ‘Mirabel’ produced less seed per pod and shorter pods after self- than after cross-pollination. No differences were noted for percent germination of seeds produced by both types of pollination indicating that the number of seeds per pod is a reliable index of the ultimate productive potential of the pollination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279707
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  • 4
    Electronic Resource
    Electronic Resource
    An isoenzyme study in the genus Lotus (Fabaceae). Experimental protocols and genetic basis of electrophoretic phenotype (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 595-607 
    Details
    ISSN: 1432-2242
    Keywords: Lotus corniculatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An isoenzyme survey of some taxa in the genus Lotus (Fabaceae) was undertaken to increase the number of genetic markers available to breeders and to students of Lotus phylogeny. Twenty-one enzymes were examined using starch gel electrophoresis and nine buffer systems. Clear, consistent banding patterns were obtained for PGI, TPI, MDH, IDH (NADP), PGM, 6-PGDH, and ME. Clear but inconsistent banding patterns were obtained for FDP, G3PDH (NADP), β-EST, LAP, MDH, DIA, and NADHDH. Phenotypes of the seven consistently resolved enzyme systems were obtained for different tissues for each of several genotypes at different stages of development. Variation in enzyme phenotypes of the same individuals under different growth conditions indicated the presence of different isozymic forms of these enzymes. Shoot tissue of plants over 6 weeks of age was found to be suitable material for further genetic studies, since phenotype for this tissue was constant despite changes in growing conditions. A formal genetic analysis of segregation and/or recombination of allozymes for the enzymes PGM, TPI, MDH, IDH, and 6-PGDH was undertaken. Isoenzyme phenotypes were examined for the diploids L. alpinus Schleich., L. burttii Sz. Borsos, L. conimbricensis Brot., L. ornithopodioides L., L. tennis Waldst. et Kit., and L. uliginosus Schkuhr; and for the diploid interspecific hybrids L. alpinus x L. conimbricensis, L. burttii x L. ornithopodioides, and L. japonicus x L. alpinus. Several new loci were identified for Lotus, namely, Idh1, Idh2, Mdh3, Pgi1, Pgi2, Tpi1, Tpi2, and 6-Pdgh1. Duplications of loci of IDH, MDH, PGI, and 6-PGDH were detected in the diploid (2n=12) interspecific hybrid L. japonicus x L. alpinus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274286
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  • 5
    Electronic Resource
    Electronic Resource
    The development of sulfonylurea herbicide-resistant birdsfoot trefoil (Lotus corniculatus) plants from in vitro selection (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 480-488 
    Details
    ISSN: 1432-2242
    Keywords: Lotus corniculatus ; Herbicide resistance ; Sulfonylurea herbicide ; In vitro selection ; Acetolactate synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Herbicide-resistant lines of birdsfoot trefoil (Lotus corniculatus L. cv ‘Leo’) were isolated after sequential selection at the callus, shoot, and whole plant levels to the sulfonylurea (SU) herbicide Harmony {DPX-M6316; 3-[[[(4-methoxy-6methyl-1,3,5, triazine-2-yl) amino] carbonyl] amino] sulfonyl-2-thiophenecarboxylate}. In field and growth chamber tests the Harmony regenerant lines displayed an increased tolerance as compared to control plants from tissue culture and controls grown from seed. Results of evaluation of callus cultures of regenerated mutant lines signify stability of the resistance. Outcrossed seeds collected from field trials, and tested in vitro for herbicide resistance, indicate that the trait is heritable and that resistance may be due to reduced sensitivity of acetolactate synthase to SU inhibition. Genetically stable herbicide-resistant lines of birdsfoot trefoil were successfully isolated using in vitro selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226537
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  • 6
    Electronic Resource
    Electronic Resource
    An isoenzyme study in the genus Lotus (Fabaceae) (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 360-368 
    Details
    ISSN: 1432-2242
    Keywords: Lotus corniculatus ; Fabaceae ; Autopolyploidy ; Allopolyploidy ; Isoenzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Segregation of the cytosolic Pgi2 locus was studied among progeny of the synthetic allotetraploid (L. japonicus × L. alpinus)2, the synthetic autotetraploid (L. alpinus)2, and the cultivated tetraploid species L. corniculatus L. Evidence of an original diploid duplication found within the interspecific hybrid L. japonicus × L. alpinus was also found within the synthetic allotetraploid (quadruplication of loci). Evidence suggesting quadruplication of loci was also found in the tetraploid L. corniculatus, but not in the synthetic autotetraploid (L. alpinus)2. It is suggested that the original duplication resulted from unequal crossing-over between homoeologues and that it provides evidence that L. corniculatus is a segmental allotetraploid. Quadruplication of loci in L. corniculatus could explain previously reported distorted tetrasomic ratios for segregation of qualitative characters in this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305829
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  • 7
    Electronic Resource
    Electronic Resource
    Evaluation of hypotheses concerning the origin of Lotus corniculatus (Fabaceae) using isoenzyme data (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 267-276 
    Details
    ISSN: 1432-2242
    Keywords: Lotus corniculatus ; Lotus species ; Fabaceae ; interspecific hybridization ; isoenzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An isoenzyme survey was conducted for several geographically dispersed accessions of four diploid Lotus species, L. alpinus Schleich., L. japonicus (Regel) Larsen, L. tenuis Waldst. et Kit and L. uliginosus Schkuhr, and for the tetraploid L. corniculatus L., in order to ascertain whether isoenzyme data could offer additional evidence concerning the origin of L. corniculatus. Seven enzyme systems were examined using horizontal starch gel electrophoresis. These were PGI, TPI, MDH, IDH, PGM, 6-PGDH, and ME. Lotus uliginosus had monomorphic unique alleles, that were not found within L. corniculatus, at 7 loci. These loci and alleles are: Tpi1-112, Pgm1,2-110, Pgm3-82, Mdh3-68, 6-Pgdh1-110, 6-Pgdh2-98,95, and Me2-100. Other diploid taxa contained alleles found in L. corniculatus for these and other loci. The implications of the isoenzyme data to theories on the origin of L. corniculatus are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257855
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  • 8
    Electronic Resource
    Electronic Resource
    Genome relationships among Lotus species based on random amplified polymorphic DNA (RAPD) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 417-422 
    Details
    ISSN: 1432-2242
    Keywords: Lotus corniculatus ; Lotus species ; Fabaceae ; interspecific hybridization ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ability of random amplified polymorphic DNA (RAPD) to distinguish among different taxa of Lotus was evaluated for several geographically dispersed accessions of four diploid Lotus species, L. tennis Waldst. et Kit, L. alpinus Schleich., L. japonicus (Regel) Larsen, and L. uliginosus Schkuhr and for the tetraploid L. corniculatus L., in order to ascertain whether RAPD data could offer additional evidence concerning the origin of the tetraploid L. corniculatus. Clear bands and several polymorphisms were obtained for 20 primers used for each species/accession. The evolutionary pathways among the species/accessions presented in a cladogram were expressed in terms of treelengths giving the most parsimonious reconstructions. Accessions within the same species grouped closely together. It is considered that L. uliginosus which is most distantly related to L. corniculatus, may be excluded as a direct progenitor of L. corniculatus, confirming previous results from isoenzyme studies. Lotus alpinus is grouped with accessions of L. corniculatus, which differs from previous studies. With this exception, these findings are in agreement with previous experimental studies in the L. corniculatus group. The value of the RAPD data to theories on the origin of L. corniculatus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223654
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  • 9
    Electronic Resource
    Electronic Resource
    Chromosome differentiation in diploid species of Lotus (Leguminosae) (1972)
    Springer
    Theoretical and applied genetics 42 (1972), S. 34-40 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiotic chromosome behavior was studied in seven diploid species of Lotus (L. alpinus Schleich., L. japonicus (Regel) Larsen, L. filicaulis Dur., L. schoelleri Schweinf., L. krylovii Schischk. and Serg., L. tenuis Waldst. et Kit., L. corniculatus var. minor Baker) and in 51 interspecific hybrids from 16 different crosses. Meiosis in the diploid species was quite regular. In a high proportion of the PMC's of the hybrids there was close chromosome homology with a normal association of 6 II's. However, meiotic irregularities including bridges, lagging chromosomes, univalents, and quadrivalents, occurred in a small percentage of the cells. The late separation of bivalents, the presence of quadrivalents, and inversion bridges with fragments, would indicate for some hybrids that certain chromosomes were structurally differentiated. The large number of rod bivalents observed at diakinesis was also highly suggestive that genetic nonhomology in one chromosome arm could contribute to the frequency of this type of bivalent. Therefore, the maximum number of 6 II's which occurred in a high percentage of cells may be misleading in that cryptic structural differences between chromosome arms, or segments, are not revealed. Pollen fertility in the species and hybrids was not correlated with meiotic irregularities suggesting that pollen fertility is genotypically controlled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306076
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  • 10
    Electronic Resource
    Electronic Resource
    Interspecific hybridization between diploid species of Lotus (Leguminosae) (1971)
    Springer
    Genetica 42 (1971), S. 353-367 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An interspecific hybridization study has been carried out between seven diploid species of Lotus (L. alpinus Schleich., L. japonicus (Regel) Larsen, L. filicaulis Dur., L. schoelleri Schweinf., L. krylovii Schischk. and Serg., L. tenuis Waldst. et Kit., and L. corniculatus var. minor Baker) closely related to L. corniculatus L. A total of 139 interspecific hybrids were produced in 16 combinations of the 7 species. Nine of these crosses were produced for the first time and four were obtained by means of embryo-culture. The growth habit, number of florets per umbel, flower color expression, HCN reaction and 15 metrical traits were compared between parents and hybrids. The relative case with which some hybrids were produced suggested that during the early evolutionary history of the genus species diversification could have originated through interspecific hybridization and subsequent gene differentiation. In some crosses, the hybrids resembled one parent more closely than the other. This close morphological affinity between the hybrids and one of their parents would make it extremely difficult to detect such hybrids in natural populations and probably aceounts for the prevailing belief that there is little or no hybridization in nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00123329
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