ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Introduction: The cellular phases (granulation, reepithelialization, and dermal remodelling) of the healing process involve many cell types. Fibroblasts and myofibroblasts are the key cells in granulation tissue formation and wound contraction.Objective: To compare the effects on cultured human fibroblasts of a new nonadhesive lipidocolloid wound dressing, Urgotul®, with five other wound dressings including impregnated gauzes and some other modern wound dressings.Method: Cultures in monolayer were used to study the morphology and growth of fibroblasts. The Bell model of cultured dermis equivalents was used to investigate myofibroblast differentiation. These cultures were labelled α-SM actin and F-actin.Results: Two of the tested dressings induced cytotoxic effects on the fibroblasts. They were found to inhibit cell growth (greater than 60%) and to disturb cell shape and cytoskeletal differentiation. Urgotul® and the remaining three dressings showed no effect on proliferation. However some of them modified fibroblast morphology and affected F-actin distribution.Conclusion: Depending on their nature and components, wound dressings may respect or affect in vitro fibroblast behaviour (proliferation, morphology, and α-SM actin and F-actin distribution). The observed in vitro findings require further investigations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2005.130117o.x
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