ISSN:
1612-1112
Keywords:
Opium alkaloids
;
Reversed-phase
;
Ion-pair
;
HPLC
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new procedure for the rapid analysis of four major opium alkaloids is described using ion-pair, reversedphase high performance liquid chromatography and recently developed radial compression column technology. The solvents were aqueous 10mM potassium perchlorate with 5.0mM n-butylamine (pH 3.0) and pure acetonitrile. A gradient was run for five minutes from 10% to 70% acetonitrile and held at that level for two minutes. Flow rates were 3 ml/min. Two column packing materials were compared with this solvent system: standard C-18 packing and a fully-end-capped C-18 material. Results obtained with the fully-end-capped C-18 packing and the two solvent ion-pair gradient showed elution times under six minutes, with greater sensitivity (〈50 nanograms) than previous systems.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268463
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