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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytica Chimica Acta 292 (1994), S. 107-112 
    ISSN: 0003-2670
    Keywords: Acetaminophen ; Amperometry ; Catalytic oxidation ; Chemically modified electrodes ; Dicyanobis(1,10-phenanthroline)iron(II) ; Flow injection ; Liquid chromatography ; Pharmaceuticals
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The molecular mechanisms involved in the invasion of Aeromonas hydrophila (strain PPD 134/91) into host cells were studied in vitro using a carp epithelial cell line. Bacterial fractions were extracted with potassium thiocyanate (KSCN) to investigate the adhesins involved. Two groups of adhesins were found. The major group was high molecular weight proteins with the largest component being a 43-kDa protein. Amino terminal sequence analysis indicated that this may have been an outer membrane porin. This supports previous suggestions that a 43-kDa outer membrane protein may be important in adhesion of a human isolate of A. hydrophila. The minor group of adhesins were low molecular weight proteins likely to be less effective in mediating bacterial adhesion and invasion into carp epithelial cells.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Blue gourami, Trichogaster trichopterus (Pallas), were intraperitoneally immunized with major adhesin, a 43 kDa OMP protein isolated from fish Aeromonas hydrophila, in the presence of Freund's complete adjuvant (FCA). Three weeks later, a booster injection of adhesin without FCA was administered. Control group fish were similarly treated with phosphate-buffered saline (PBS) and FCA. Results showed that anti-adhesin serum obtained from fish after booster immunization exhibited very strong ability in agglutinating bacterial cells. Although this antiserum had no bactericidal effect, it could significantly inhibit serologically different strains of A. hydrophila from invading EPC (Epithelioma papillosum of carp) cells in vitro. In addition, the proliferative response of head kidney leucocytes of these immune fish was significantly increased as compared to that of the control. The results also showed that the major adhesin could provide significant protective immunity to fish against the challenge by homologous and heterologous strains of A. hydrophila and one virulent strain of Vibrio anguillarum.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of optimization theory and applications 54 (1987), S. 241-252 
    ISSN: 1573-2878
    Keywords: Global minimizer ; basin (region of attraction) of a minimizer ; lower minimizer ; higher minimizer ; filled (modified) function method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper is concerned with filled function methods for finding global minimizers of a function of several variables. A class of filled functions is defined. The advantages and disadvantages of every filled function in the class are analyzed. The best one in this class is pointed out. The idea behind constructing a better filled function is given and employed to construct the class of filled functions. A method is also explored on how to locate minimizers or saddle points of a filled function through only the use of the gradient of a function.
    Type of Medium: Electronic Resource
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