ISSN:
1432-072X
Keywords:
Paracoccus denitrificans
;
Ammonia assimilation pathways
;
Nitrogen catabolism regulation
;
Ammonium transport
;
Glutamine synthetase
;
Glutamate synthase
;
Aminotransferase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A Tn5 insertional prototrophic mutant of Paracoccus denitrificans (UBM219) was generated which grew on high (〉1 mM) but not low (〈0.5 mM) ammonium as sole nitrogen source. It did not utilize nitrate and most amino acids except glutamate and aspartate. UBM219 showed more than 10-fold lower levels of ammonium (methylammonium) transport, aspartate and alanine aminotransferase, but more than 10-fold higher activities of glutamate dehydrogenase and glutamate synthase. This pleiotropy indicates a mutation in a regulatory gene affecting nitrogen metabolism in general. — Ammonia assimilation pathways and regulation in Paracoccus resemble the patterns in enterobacteria with the exception, that alanine is generated by amino transfer from glutamate to pyruvate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00288721
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