ISSN:
0935-6304
Keywords:
Chromatographic separation techniques
;
High Performance Liquid Chromatography (HPLC)
;
Gel Permeation Chromatography (GPC)
;
Supercritical Fluid Chromatography (SFC)
;
Capillary HPLC
;
Nuclear Magnetic Resonance (NMR) spectroscopy
;
hyphenation of chromatography with NMR spectroscopy
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
---The hyphenation of chromatographic separation techniques with NMR spectroscopy is one of the most powerful and time-saving methods for the separation and structural elucidation of unknown compounds and molecular compositions of mixtures. Most of the routinely used NMR flow-cells have detection volumes between 40-180 μL for conventional separations with analytical columns, and the newest designs employ detection volumes in the order of 200 nL for capillary separations. The low flow rates used in capillary chromatography permit the use of deuterated solvents. Unequivocal structural assignment of unknown chromatographic peaks is possible by two-dimensional stopped-flow capillary HPLC-NMR experiments.
Additional Material:
14 Ill.
Type of Medium:
Electronic Resource
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