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  • 1
    ISSN: 1432-0428
    Keywords: Keywords Chylomicron composition ; apolipoprotein B48 ; microsomal triglyceride transfer protein.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. The aim of this study was to examine factors involved in chylomicron production in the streptozotocin diabetic rat, our hypothesis being that the synthesis of the chylomicron is abnormal in diabetes. Methods. Diabetic rats (n = 20) were paired with control rats (n = 20). Cholesterol emulsion was given by gavage and the lymph duct was cannulated. Lymph was collected for 4 h. Chylomicrons were prepared from the lymph by ultracentrifugation. Lymph apolipoprotein B48 was isolated by gradient gel electrophoresis and quantified by densitometric scanning. Intestinal microsomal triglycerol transfer protein mRNA was measured by solution hybridisation nuclease protection, using a rat specific [32P]-labelled cRNA probe. Results. Serum triglyceride and cholesterol were greatly increased in diabetic compared with control animals (258 ± 77 and 8.9 ± 6.4 mg/ml vs 1.04 ± 0.37 and 0.54 ± 0.03 mg/ml, p 〈 0.0001). Lymph chylomicron triglyceride and cholesterol were also higher in diabetic rats (29.4 ± 27.3 and 0.28 ± 0.3 mg/h vs 16.8 ± 10.6 and 0.18 ± 0.09 mg/h, p 〈 0.05). Lymph chylomicron apo B48 was similar in the two groups. Intestinal microsomal triglycerol transfer protein mRNA was higher in the diabetic rats (12.6 ± 3.2 vs 3.8 ± 3.0 amol/μg RNA, p 〈 0.0001) and there was a positive correlation between lymph triglyceride and microsomal triglycerol transfer protein mRNA in the whole group (r = 0.65, p 〈 0.01). Conclusion/interpretation. The study shows that microsomal triglycerol transfer protein mRNA is raised in diabetes without an increase in apolipoprotein B48 in the lymph suggesting that microsomal triglycerol transfer protein regulates chylomicron triglyceride content but not particle number. [Diabetologia (1999) 42: 944–948]
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 42 (1987), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sowing and post-sowing management techniques were assessed for their effectiveness in introducing Trifolium repens and T. pratense into an established Phalaris aquatica sward in spring and autumn.Percentage emergence, seedling survival, seedling weight and final clover content were greater with T. pratense than with T. repens. Surface sowing gave 10% emergence compared to 62% for tilled treatments. Rotary sowing gave superior emergence in autumn, while tined sowing gave superior emergence in spring. However, seedling survival and weight, and therefore final clover content, were generally greater with rotary sowing than with tined sowing.The removal of competition from the resident grass by bandspraying with glyphosate prior to sowing, and repeated lenient defoliation postsowing, increased final seedling numbers by 19% and 46%, respectively, and their individual weight by 29% and 82%, respectively.The results showed that direct sowing in spring or autumn can be used to restore successfully a substantial population of clover plants to an existing perennial grass sward. They also demonstrated the importance of management to manipulate the environment of the developing clover plant.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 45 (1990), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: White clover, (cv. Haifa) and subterranean clover (cv. Seaton Park) were grown in binary replacement series mixtures in the field at proportions of 100:0, 90:10, 50:50. 10:90 and 0:100, and cut every four weeks over three growing seasons from 1985 to 1987 at Scone, New South Wales (NSW) (32°S).Relatively low plant densities minimized competition in 1985, but in subsequent seasons Haifa and Seaton Park competed vigorously for the same resource;.; usually having relative crowding coefficients greater than 1.0, and relative yield totals greater than 1.0 in spring of 1986 and 1987, and autumn 1987. This over-yielding appeared to be due to some complementarity of leaf morphology and canopy architecture.Haifa severely depressed seed production of Seaton Park in mixtures. Haifa regenerated as an annual in 1986, but as a perennial, from surviving stolons, in 1987 owing to lower maximum temperatures and better rainfall distribution in late summer and autumn of 1987.It is concluded that white clover can survive severe competition from subterranean clover as a seedling, and if moisture allows, may severely depress seed production from subterranean clover, regardless of whether plants are annual or perennial in origin. Spatial separation may minimize competition in average years but competition effects may be severe in seasons with sufficient summer rainfall for perennation of white clover, and seasonal autumn rainfall for germination of subterranean clover.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 43 (1988), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Phalaris, subterranean clover and white clover were grown together in binary, diallel replacement series mixtures at (day/night) 15/10 or 24/19°C under long-day conditions, and cut at 2- or 4-weekly intervals. Analysis of white clover mixtures was confined to 15/10°C due to losses of seedlings prior to imposition of treatments at 24/19°C.With white clover at 15/10°X, seedling losses occurred after the first harvest, the greater losses occurring in mixtures where subterranean clover was the major component. The de Wit analyses showed that subterranean clover excluded its companions, whereas phalaris and white clover competed for slightly different niches resulting in over-yielding. Gleeson McGilchrist analyses showed that subterranean clover and phalaris were more aggressive than white clover under infrequent cutting, but that white clover was more aggressive as a major than as a minor component of a mixture under frequent cutting. Between phalaris and subterranean clover [he pattern of competition at 24/19°C differed from that at 15/10°C only in the more rapid development of subterranean clover and consequent swing to phalaris dominance.We conclude that the seedlings of white clover and subterranean clover are suited to combination with phalaris because phalaris is not too aggressive towards white clover, and is tolerant of the aggressiveness of subterranean clover. The survival of only a few seedlings of white clover in mixtures with subterranean clover may be sufficient for long-term growth provided some perennation occurs after the senescence of subterranean clover.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 31 (1998), S. 881-889 
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: A new instrument for simultaneous small-angle X-ray scattering and rheology experiments on soft solids is described. This device is based on a commercial rheometer with a shear sandwich geometry in which the sample is subjected to a planar oscillatory deformation. This instrument has been used for time-resolved small-angle X-ray scattering/rheology experiments at the Synchrotron Radiation Source, Daresbury Laboratory, England. The focus has been in particular on the effect of large-amplitude shearing on the orientation of cubic phases in gels of block copolymers formed in concentrated solutions, and on the bicontinuous cubic phase of a block copolymer melt. Representative results are presented for face-centred cubic (f.c.c.) and body-centred cubic (b.c.c.) phases in gels of poly(oxyethylene)–poly(oxybutylene) diblock copolymers, and for the bicontinuous cubic `gyroid' structure in a poly-(ethylene-alt-propylene)–poly(dimethylsiloxane) di-block copolymer melt. The orientations of the micellar b.c.c. phases in the gels and the gyroid structure (belonging to the b.c.c. space group Ia\bar 3d) following large-amplitude shearing are shown to be the same, i.e. directionally oriented crystals are produced in both cases, in which (111) directions are oriented along the shear direction.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sheep x hamster cell hybrids containing sheep metacentric Chromosome (Chr) 2 were produced by fusing blood leukocytes from normal sheep with hamster auxotrophic Ade F-minus mutants. Cell clones that were isocitrate dehydrogenase 1 (IDH1) positive were cytogenetically characterized, confirming that they contained sheep Chr 2. The following loci were newly assigned by Southern hybridization to sheep Chr 2: lipoprotein lipase (LPL), glycoprotein-4-beta galactosyltransferase 2 (GGTB2), neurofilament light polypeptide (68 kDa; NEFL), surfactant-associated protein 2 (SFTP2), lymphocyte-specific protein tyrosine kinase (LCK), and nebulin (NEB). These new assignments and the in situ localization of gelsolin (GSN) to sheep Chr 2pter-p24 are consistent with the predicted homology of cattle Chr 8 (U18) with sheep Chr 2p, and of cattle Chr 2 (U17) with sheep 2q. In addition, the assignment by cell hybrid analysis of loci previously mapped to Chr 2 in sheep, viz., cholinergic receptor, nicotinic, delta polypeptide (CHRND), collagen type III alpha 1 (COL3A1), fibronectin 1 (FN1), isocitrate dehydrogenase (IDH1), and villin 1 (VIL1), confirmed the localization of sheep syntenic group U11 to this chromosome. By nutritional selection and complementation of the hamster auxotrophic Ade F mutation, the multifunctional enzyme locus phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase)/IMP cyclohydrolase (inosinicase) (provisionally given the symbol PRACFT) has also been newly assigned to sheep Chr 2. This report significantly extends the number of loci physically mapped to sheep Chr 2 and confirms its close homology with cattle Chrs 2 and 8.
    Type of Medium: Electronic Resource
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