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  • 1
    Electronic Resource
    Electronic Resource
    Genetics and molecular biology of Azospirillum (1999)
    Springer
    Biology and fertility of soils 29 (1999), S. 10-23 
    Details
    ISSN: 1432-0789
    Keywords: Key words Azospirillum ; Plant interaction genes ; Plasmid p90 ; Plant-growth-promoting rhizobacteria ; Promoter sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Genetic manipulation of Azospirillum spp. has facilitated a better understanding of the mode of action of this plant-growth promoting bacterium and should help to improve its ability to stimulate plant growth and development. This review considers and discusses Agospirillum plasmids, promoter sequences, the isolation of Azospirillum mutants, the genetic transformation of Azospirillum, the transfer of foreign genes into Azospirillum by conjugation and the Azospirillum genes that have been isolated and characterized. The Azospirillum genes that are discussed include genes involved in nitrogen fixation, plant root attachment, phytohormone biosynthesis, tryptophan biosynthesis, carbon metabolism and a few other less well characterized processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050519
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The production of ethanol from lactose in a tubular reactor by immobilized cells of Kluyveromyces fragilis (1986)
    Springer
    Applied microbiology and biotechnology 24 (1986), S. 277-281 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary An immobilized-cell tubular reactor for the continuous fermentation of lactose by Kluyveromyces fragilis was developed. Two types of supporting media were successfully tested; beechwood cubes and activated charcoal pellets. Ethanol productivity of 17.2 g/l/h was achieved from a 15% whey-lactose solution using K. fragilis immobilized on charcoal pellets, with a final ethanol concentration of 18 g/l. The use of two reactors in series demonstrated that it is possible to obtain up to 50 g/l of ethanol in the final product. No decrease in biological activity of the immobilized yeast cells occurred over a period of up to 31 days of continuous operation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257049
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter (1996)
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 145-154 
    Details
    ISSN: 1476-5535
    Keywords: lac promoter ; tac promoter ; recombinant DNA ; protein overexpression ; fermentation strategies ; IPTG ; lactose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This review examines factors which influence the expression of foreign proteins inEscherichia coli under the transcriptional control of thelac andtac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-β-d-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569997
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of Wild-Type and Mutant Plant Growth-Promoting Rhizobacteria on the Rooting of Mung Bean Cuttings (1999)
    Springer
    Journal of plant growth regulation 18 (1999), S. 49-53 
    Details
    ISSN: 1435-8107
    Keywords: Key Words. Ethylene—Plant growth-promoting rhizobacteria—Adventitious roots—ACC deaminase—Indole-3-acetic acid—Mung bean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Mung bean cuttings were dipped in solutions of wild type and mutant forms of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 and then incubated for several days until roots formed. The bacteria P. putida GR12-2 and P. putida GR12-2/aux1 mutant do not produce detectable levels of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, whereas P. putida GR12-2/acd36 is an ACC deaminase minus mutant. All bacteria produce the phytohormone indole-3-acetic acid (IAA), and P. putida GR12-2/aux1 overproduces it. Treatment of cuttings with the above-mentioned bacteria affected the rates of ethylene production in the cuttings in a way that can be explained by the combined effects of the activity of ACC deaminase localized in the bacteria and bacterial produced IAA. P. putida GR12-2 and P. putida GR12-2/acd36-treated cuttings had a significantly higher number of roots compared with cuttings rooted in water. In addition, the wild type influenced the development of longer roots. P. putida GR12-2/aux1 stimulated the highest rates of ethylene production but did not influence the number of roots. These results are consistent with the notion that ethylene is involved in the initiation and elongation of adventitious roots in mung bean cuttings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007047
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  • 5
    Electronic Resource
    Electronic Resource
    Development and enhancement of agricultural biotechnology in some countries in Latin America (1991)
    Springer
    World journal of microbiology and biotechnology 7 (1991), S. 164-170 
    Details
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A number of research institutions and both local and international agencles in Latin America are using biotechnology as part of an effort to enhance agricultural productivity. However, it is very much an open question as to whether all of these various organizations can provide the best means of realizing this goal. Latin American countries vary dramatically in their knowledge base and current use of modern biotechnology. Thus, while some countries lack the ability to develop, or possibly even implement, many aspects of modern biotechnology, others are quite advanced in this regard. This review provides a somewhat selective overview of current research in the area of agricultural biotechnology in Mexico, Costa Rica and Ecuador, with emphasis on how the existing agencies and institutions have responded to the challenge of biotechnology. In addition, general strategies for the development of agricultural biotechnology in these countries are presented and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328986
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    Paper (German National Licenses)
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