ISSN:
1617-4623
Keywords:
Key words Hfr donor
;
Mapping
;
Obligate methylotroph
;
Methylobacillus flagellatum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We present a refined genetic map of the obligate methylotroph Methylobacillus flagellatum. New, Hfr (high-frequency-of-transfer) donors, and pulsed-field gel electrophoresis, were used to determine that M.␣flagellatum contains one ∼3.1-Mb circular chromosome, and no plasmids. A correlation between time-of-entry units and DNA length was established. Using in vivo and in vitro cloning, and sequencing, a number of new genetic markers were identified and mapped; in addition, the nature of some of the previously mapped markers was elucidated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380050715
Permalink