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  • 1
    Electronic Resource
    Electronic Resource
    A method for determining the position and size of optimal sequence regions for phylogenetic analysis (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 1128-1138 
    Details
    ISSN: 1432-1432
    Keywords: Phylogeny ; Neighbor joining method ; Bootstrap ; Optimal size ; Optimal position ; Nonrandom sequence ; Information ; Foot-and-mouth disease virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The availability of fast and accurate sequencing procedures along with the use of PCR has led to a proliferation of studies of variability at the molecular level in populations. Nevertheless, it is often impractical to examine long genomic stretches and a large number of individuals at the same time. In order to optimize this kind of study, we suggest a heuristic procedure for detection of the shortest region whose informational content can be considered sufficient for significant phylogenetic reconstruction. The method is based on the comparison of the pairwise genetic distances obtained from a set of sequences of reference to those obtained for different windows of variable size and position by means of a simple index. We also present an approach for testing whether the informative content in the stretches selected in this way is significantly different from the corresponding content shown by the larger genomic regions used as reference. Application of this test to the analysis of the VP1 protein gene of foot-and-mouth-disease type C virus allowed us to define optimal stretches whose informative content is not significantly different from that displayed by the complete VP1 sequence. We showed that the predictions made for type C sequences are valid for type O sequences, indicating that the results of the procedure are consistent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173194
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Larval competition in Drosophila melanogaster: effects on development time (1990)
    Springer
    Genetica 82 (1990), S. 33-44 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects on development time of larval competition that is interrupted at different times are analyzed in an experiment with constant larval density and varying proportions of two competing strains. Interruption of competition is carried out by means of the overfeeding technique, which allows for the study of individuals that, after several days under competition stress, either complete their development in crowded conditions or migrate to a vial with fresh food where the rest of their development takes place. Results of mean development time and within-vial variance of development time show that individuals under strong competition stress can arrest their development retaining the capability to resume it, once they are exposed to fresh food. The effects of competition are observed sooner on development time than on viabilities, which is in agreement with Ohba's observations (Biol. J. Okayama Univ. 7: 87–125, 1961), but the observed effects are not always coincident for both fitness components.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00057671
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Multiple methods for the identification of polymorphic simple sequence repeats (SSRs) in sorghum [Sorghum bicolor (L.) Moench] (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 190-198 
    Details
    ISSN: 1432-2242
    Keywords: Microsatellite ; Germplasm ; Genetic resources ; Genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simple sequence repeats (SSRs), also known as microsatellites, are highly variable DNA sequences that can be used as markers for the genetic analysis of plants. Three approaches were followed for the development of PCR primers for the amplification of DNA fragments containing SSRs from sorghum [Sorghum bicolor (L.) Moench]: a search for sorghum SSRs in public DNA databases; the use of SSR-specific primers developed in the Poaceae species maize (Zea mays L.) and seashore paspalum grass (Paspalum vaginatum Swartz); and the screening of sorghum genomic libraries by hybridization with SSR oligonucleotides. A total of 49 sorghum SSR-specific PCR primer pairs (two designed from GenBank SSR-containing sequences and 47 from the sequences of genomic clones) were screened on a panel of 17 sorghum and one maize accession. Ten primer pairs from paspalum and 90 from maize were also screened for polymorphism in sorghum. Length polymorphisms among amplification products were detected with 15 of these primer pairs, yielding diversity values ranging from 0.2 to 0.8 with an average diversity of 0.56. These primer pairs are now available for use as markers in crop improvement and conservation efforts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225745
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Multiple methods for the identification of polymorphic simple sequence repeats (SSRs) in sorghum [Sorghum bicolor (L.) Moench] (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 190-198 
    Details
    ISSN: 1432-2242
    Keywords: Key words Microsatellite ; Germplasm ; Genetic resources ; Genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simple sequence repeats (SSRs), also known as microsatellites, are highly variable DNA sequences that can be used as markers for the genetic analysis of plants. Three approaches were followed for the development of PCR primers for the amplification of DNA fragments containing SSRs from sorghum [Sorghum bicolor (L.) Moench]: a search for sorghum SSRs in public DNA databases; the use of SSR-specific primers developed in the Poaceae species maize (Zea mays L.) and seashore paspalum grass (Paspalum vaginatum Swartz); and the screening of sorghum genomic libraries by hybridization with SSR oligonucleotides. A total of 49 sorghum SSR-specific PCR primer pairs (two designed from GenBank SSR-containing sequences and 47 from the sequences of genomic clones) were screened on a panel of 17 sorghum and one maize accession. Ten primer pairs from paspalum and 90 from maize were also screened for polymorphism in sorghum. Length polymorphisms among amplification products were detected with 15 of these primer pairs, yielding diversity values ranging from 0.2 to 0.8 with an average diversity of 0.56. These primer pairs are now available for use as markers in crop improvement and conservation efforts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050265
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Larval competition in Drosophila melanogaster: frequency-dependence of viability (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 366-377 
    Details
    ISSN: 1432-2242
    Keywords: Larval competition ; Larva-to-adult viability ; Frequency-dependent selection ; Overfeeding technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The application of the overfeeding technique (interruption of the competition during larval development) to the study of larval competition in two-strain cultures of Drosophila melanogaster demonstrates the following points: (1) viability is a function of competition time; (2) viability becomes more frequency-dependent as competition time increases; (3) the dynamics of the “inner” subpopulation (adults that have passed all their development in a crowded condition) and “outer” subpopulation (adults coming from larvae recovered by interruption of competition) vary with time as regards frequency-dependence; and (4) the wild type strain Oregon is the active agent in competition with the strain cardinal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303979
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Intra- and intergenotypic competition in Drosophila melanogaster: effects of density on larval survival and rate of development (1986)
    Springer
    Genetica 70 (1986), S. 59-67 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the effects of density and frequency in the larval competition of Drosophila melanogaster by measuring three fitness components: viability (V), mean development time (MDT) and a combination of these two (E). We have detected (contrary to most published results) non-linear effects of density in single-genotype cultures; in addition, different functions are required to describe the density effects below and above the optimal density. Frequency has also non-linear effects in the two-genotype cultures. Only one polymorphic equilibrium frequency, which is stable, occurs with respect to V; but two polymorphic equilibria, one stable and one unstable, exist with respect to E. The responses in single-genotype cultures do not allow one to predict the outcome of the competition in two-genotype cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00123215
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    Paper (German National Licenses)
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