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  • 1
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract: A3 adenosine receptors have been proposed to play an important role in the pathophysiology of cerebral ischemia with a regimen-dependent nature of the therapeutic effects probably related to receptor desensitization and down-regulation. Here we studied the agonist-induced internalization of human A3 adenosine receptors in transfected Chinese hamster ovary cells, and then we evaluated the relationship between internalization and signal desensitization and resensitization. Binding of N6-(4-amino-3-[125I]iodobenzyl)adenosine-5′-N-methyluronamide to membranes from Chinese hamster ovary cells stably transfected with the human A3 adenosine receptor showed a profile typical of these receptors in other cell lines (KD = 1.3 ± 0.08 nM; Bmax = 400 ± 28 fmol/mg of proteins). The iodinated agonist, bound at 4°C to whole transfected cells, was internalized by increasing the temperature to 37°C with a rate constant of 0.04 ± 0.034 min-1. Agonist-induced internalization of A3 adenosine receptors was directly demonstrated by immunogold electron microscopy, which revealed the localization of these receptors in plasma membranes and intracellular vesicles. Moreover, short-term exposure of these cells to the agonist caused rapid desensitization as tested in adenylyl cyclase assays. Subsequent removal of the agonist led to restoration of the receptor function and recycling of the receptors to the cell surface. The rate constant of receptor recycling was 0.02 ± 0.0017 min-1. Blockade of internalization and recycling demonstrated that internalization did not affect signal desensitization, whereas recycling of internalized receptors was implicated in the signal resensitization.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 188 (1980), S. 107-113 
    ISSN: 1432-041X
    Schlagwort(e): Planarian regeneration ; Cell transdifferentiation ; DNA Cytophotometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The role and fate of male germ cells in planarian regeneration was studied in a population ofDugesia lugubris s.1. which provided a suitable karyological marker to distinguish diploid male germ cells from triploid embryonic and somatic cells. The nuclear Feulgen-DNA content in non-replicating triploid muscle cells of the pharynx and in non-replicating male gonia of testes from intact animals were measured by the cytophotometric technique. The pharynx was then removed by transection and each anterior regenerant was allowed to completely regenerate this organ. Measurements of the Feulgen-DNA content in muscle cells of the regenerated pharynx showed that most of these cells (∼95%) have a DNA content typical of triploid cells; however, some muscle cells (∼5%) with a nuclear DNA content typical of male gonia alone were observed. These results were interpreted in the following way. After transection, young male germ cells move from the testes to the wound where they participate in blastema formation along with reserve and/or somatic dedifferentiated cells. During regeneration some of these cells of male origin differentiate into pharyngeal muscle cells. Our findings are discussed in relation to the occurrence of mataplasia in planarians.
    Materialart: Digitale Medien
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  • 3
    ISSN: 1432-234X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary European populations of the turbellarianMesostoma ehrenbergii can produce two types of eggs according to a seasonal cycle. Subitaneous eggs (S eggs) are produced throughout the warm season and develop rapidly inside the parental uteri. They are meant to ensure a fast increase in the number of worms. Dormant eggs (D eggs) are produced during the warm season and are usually deposited in water at the death of the parent. They can hatch at the beginning of the next warm season and are, therefore, meant to ensure the survival of the population throughout the cold season, when adult worms are no longer present. S eggs are bounded by a thin, translucent covering which has a loose structure and is mainly proteinaceous in composition. These characteristics account for the functional role played by the S egg covering during embryonic development. The covering has, in fact, to be permeable to parental nutrients, and to keep pace, by stretching, with the enlargement of the embryo. By contrast, D eggs are bounded by a thick shell, presumably composed of a sclerotin, the precursors of which (polyphenols) are synthesized together with yolk in the vitelline cells. The shell has a smooth surface devoid of pores and has the function of isolating the developing D egg from the external environment.
    Materialart: Digitale Medien
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  • 4
    ISSN: 1432-234X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The unpaired germarium of Dicrocoelium dendriticum contains many female germ cells at different stages of maturation and is enveloped by a fibrous basal lamina-like structure and a multilayered cytoplasmic sheath whose origins and functions are discussed. The maturation process of primary oocytes occurs completely within the prophase of the first meiotic division. It has been divided into three stages, as previously suggested for monogeneans. Stage I corresponds to oogonia and early oocytes which are located in the distal germinative area of the gonad. These cells are characterized by a high nucleo/cytoplasmic ratio and a poorly differentiated cytoplasm. Stage II corresponds to maturing oocytes grouped in the central area of the gonad and exhibiting long synaptonemal complexes and a prominent nucleolus. The main feature of cytoplasmic differentiation is the increase in the number of RER and Golgi complex which are involved in the production of small electron-dense granules. Stage III corresponds to mature oocytes located in the proximal area of the germarium near the origin of the oviduct. In this stage, the granules become regularly distributed in a monolayer in the peripheral ooplasm and make contact with the oolemma. They show a distinctive complex structure, are composed of proteins and glycoproteins and do not contain polyphenols. Their possible role as cortical granules is discussed in relation to chemical composition and previous studies on other Plathelminthes. Neither yolk globules nor glycogen are present in the oocytes.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Hydrobiologia 305 (1995), S. 207-212 
    ISSN: 1573-5117
    Schlagwort(e): Rhabdocoela ; Platyhelminthes ; germanium ; ovary ; oocyte ; cortical inclusions ; ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Ultrastructure features of the germanium (ovary) have been investigated in several species of Rhabdocoela. The gonad is usually unpaired, small and pear-shaped; it is enveloped by an extracellular lamina and contains oocytes at different stages of maturation. Elongate accessory cells surround germ cells in the peripheral zone of the gonad and can also fill the internal spaces between oocytes with their long, flattened processes. The main features observed during oocyte maturation were the appearance of chromatoid bodies, annulate lamellae, lipid droplets and glycogen particles, and the development of R.E.R. and Golgi complexes which appeared correlated with the production of small inclusions that became localized in the cortical ooplasm of mature germ cells. The inclusions exhibited a different structure in different taxa and contained variable amounts of polyphenols. Cortical inclusions of rhabdocoels most probably represent residual shell-granules and may participate in the capsule-shell formation.
    Materialart: Digitale Medien
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  • 6
    ISSN: 1573-5117
    Schlagwort(e): Platyhelminthes ; Lecithoepitheliata ; female gonad ; oocytes ; vitellocytes ; ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The female gonad of Prorhynchus is heterocellular (neoophoran organization) and consists of an unpaired, elongate germovitellarium enveloped by a finely granular extracellular lamina. It is composed of a posterior germinative area where early oocytes are randomly associated with differentiating vitellocytes and a growth area with follicular organization. In each follicle a single oocyte is surrounded by a layer of vitellocytes. By electron microscopy, the oocytes showed features typical of non-vitellogenic germ cells; they had chromatoid bodies, annulate lamellae, lipid droplets and R.E.R. and Golgi complexes producing small granules with a multilamellar pattern. Vitellocytes showed features typical of secretory cells with the R.E.R. and Golgi complex developed to a great extent and involved in the production of type A and type B globules, respectively. We speculate that type A globules are shell-globules and type B globules are yolk. The structure, composition and role of vitellocyte globules of Prorhynchus are compared with those of homologous inclusions from other Platyhelminthes.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Hydrobiologia 227 (1991), S. 105-111 
    ISSN: 1573-5117
    Schlagwort(e): Platyhelminthes ; Turbellaria ; vitelline cells ; shell-globules ; gonad ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The Neoophora is characterized by the presence of complex female gonads composed of both germaria and vitellaria. The vitellaria are made up of vitelline cells that differentiate to produce and accumulate reserve substances (proteins, lipid, glycogen) and precursors of the egg capsule or cocoon shell (phenolic proteins). A comparative ultrastructural and cytochemical investigation of shell-forming globules from the vitelline cells of some neoophoran platyhelminths shows that the internal structures of the shellforming globules can be grouped into two (or three) main types. In the first, globule content is characterized by a more or less homogeneous electron-dense substructure evidently arising from repeated coalescence of small vesicles produced by the rough endoplasmic reticulum and Golgi complex. A variant of this type of globule shows intermingling (or concentric) electron-dense and translucent areas producing a pattern resembling brain convolutions (‘convoluted pattern’). The second type of shell-globule structure shows a ‘multigranular pattern’, presumably resulting from repeated fusions of Golgian vesicles followed by incomplete coalescence of the electron-dense content. Comparison of shell-globule structure in different taxa could be useful in elucidating some complex and still-unclear phylogenetic relationships among Platyhelminthes.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Hydrobiologia 383 (1998), S. 215-226 
    ISSN: 1573-5117
    Schlagwort(e): Platyhelminthes ; Temnocephalida ; female gonad ; ultrastructure ; cytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The female gonad of Temnocephala dendyi and T. minor consists of a single germarium and two rows of vitellaria. It is enveloped by an extracellular lamina and accessory cells. Accessory cells are only peripherally located in the germarium while their cytoplasmic projections also fill the spaces between vitellocytes in the vitellarium. The main feature of oocyte maturation is the appearance of chromatoid bodies and the development of rough endoplasmic reticulum (R.E.R.) and Golgi complexes which appear to be correlated with the production of double-structured egg granules. The egg granules, which are localized in the cortical cytoplasm of mature oocytes, contain glycoproteins, are devoid of polyphenols and are similar in structure and composition to the cortical granules observed in some Digenea and Monogenea. Vitellocytes are typical secretory cells with well-developed R.E.R. and Golgi complexes which are involved in the production of shell globules and yolk. The multigranular pattern and the polyphenolic composition of the shell globules of the temnocephalids investigated are similar to those observed in other rhabdocoels, and in some Prolecithophora and Neodermata. This feature may represent a synapomorphy shared by these taxa.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Hydrobiologia 84 (1981), S. 171-179 
    ISSN: 1573-5117
    Schlagwort(e): Turbellaria ; Tricladida ; cell differentiation ; regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Journal of Morphology 149 (1976), S. 265-277 
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Oogenesis of the fresh-water triclad Dugesia dorotocephala has been studied by electron microscopical methods, with particular regard to the genesis and composition of the so-called “Balbiani body.” Its origin is clearly recognizable in young oocytes where the few mitochondria present seem to gather at the level of the perinuclear ooplasm. Here they surround dense masses of finely granular, fibrillar material probably coming from the nucleus. During the previtelloge ic period, mitochondria rapidly increase in number while the dense masses progressively dissolve.In the vitellogenic oocytes the Balbiani body shows its final configuration: it appears as a large area (up to 15-20 pm in diameter) consisting of innumerable densely packed mitochondria, some smooth vesicles and free ribosomes. This aggregate of cytoplasmic organelles remains unmodified in the mature oocytes.The function of the “Balbiani body” of D. dorotocephala is as yet unclear; it can only be asserted that it is not correlated with yolk production in which the endoplasmic reticulum and the Golgi complex are involved.
    Materialart: Digitale Medien
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