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  • 1
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in Vicia faba L. (1987)
    Springer
    Plant cell, tissue and organ culture 9 (1987), S. 167-171 
    Details
    ISSN: 1573-5044
    Keywords: Vicia faba L. ; callus culture ; suspension culture ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The paper describes a method of somatic embryo induction in callus and suspension cultures of Vicia faba L. Callus was induced from immature cotyledons (green maturity stage) of white-flowering horse bean lines cultured on L2 medium (Phillips and Collins 1979) supplemented with 1% sucrose, 0.7% agar and different concentrations of 2,4-dichlorophenoxyacetic acid. The medium with 2.5 μM 2,4-Dichlorophenoxyacetic acid was found optimum for embryogenic callus induction. Somatic embryos developed after transfer of the callus to media lower or zero 2,4-Dichlorophenoxyacetic acid and increased level of sucrose (2.5%). The release of somatic embryos from the callus was more apparent after transfer to liquid medium. There were various stages of somatic embryo development, i.e. globular, heart-shaped and torpedo ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044253
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Utilization of isozyme polymorphism for cultivar identification of 45 commercial peas (Pisum sativum L.) (2000)
    Springer
    Euphytica 113 (2000), S. 249-256 
    Details
    ISSN: 1573-5060
    Keywords: cultivar identification ; electrophoresis ; isozymes ; pea ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Forty five Pisum sativum cultivars were analysed by isozyme electrophoresis with the objective to find protein markers for exact and reproducible discrimination of individual genotypes. The combination of six enzyme systems (acid phosphatase, amylase, esterase, leucine aminopeptidase, shikimate dehydrogenase and phosphoglucomutase) with two electrophoretic techniques (NATIVE-PAGE, isoelectric focusing) and use of seed and leaf tissue enabled to identify all 45 studied cultivars. Critical factors which may affect utilization of isozyme electrophoresis for commercial applications in pea breeding and seed production and testing are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003967315213
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of tissue culture-derived variation in pea (Pisum sativum L.)-preliminary results (1955)
    Springer
    Euphytica 85 (1955), S. 335-339 
    Details
    ISSN: 1573-5060
    Keywords: callus culture ; organogenesis ; pea ; Pisum sativum ; somaclonal variation ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The possibility of producing agronomically-useful somaclones via organogenesis and somatic embryogenesis from callus cultures of pea (Pisum sativum L.) was studied. Organogenic calli were induced from immature leaflets on MSB medium with NAA and BAP. Embryogenic calli were derived either from immature zygotic embryos (using 2,4-D) or from shoot apices (using picloram) of aseptically-germinated seedlings. The seed progenies (T1 to T3-generation) of primary regenerants were grown in field conditions and their phenotypic variation was evaluated and compared with control, non-tissue culture-derived plant material. In addition, electrophoretic analyses of selected isoenzyme systems and total proteins have been done. The results do not show dramatic changes in qualitative and quantitative traits. The evaluation of at least two future generations (T4, T5) is planned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023964
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro clonal propagation of Pisum sativum L. (1986)
    Springer
    Plant cell, tissue and organ culture 6 (1986), S. 95-104 
    Details
    ISSN: 1573-5044
    Keywords: Pisum sativum L. ; pea ; shoot tip culture ; axillary bud culture ; regeneration ; organogenesis ; in vitro clonal propagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A system of in vitro clonal propagation has been developed in Pisum sativum L. (cv. Bohatýr). A modified MS-medium supplemented with 20 μM 6-benzylaminopurine (BAP) and 0.1 μM α-naphthaleneacetic acid (NAA) was used to induce multiple shoot formation from shoot apices, axillary buds of the first normal leaf, axillary buds of the first and second primary scales and axillary buds of cotyledons of 4 to 6 day old pea seedlings. Meristem explants maintained a high proliferation ability in each subculture in the course of 20 months of the culture. Regenerated shoots were rooted in the same basal medium containing 5 μM NAA. Rooted plants were cultured in hydroponic pots filled with half-strength MS-medium to attain anthesis and seed maturity. The phenotypic uniformity of the regenerants was evaluated. Cytological investigation confirmed the diploid stage (2n=14) of regenerants and their progeny. Histological studies revealed that proliferating shoots originated from axillary and adventitious buds. In vitro propagation is discussed as related to pea breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037762
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    Paper (German National Licenses)
    Fulltext
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