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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of RNA–Mediated Resistance to Tomato Spotted Wilt Virus in Transgenic Tobacco Plants (1992)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 10 (1992), S. 1133-1137 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Recently high levels of protection against tomato spotted wilt virus (TSWV), a negative–strand RNA virus infecting plants, have been obtained by transforming tobacco with viral nucleoprotein (N) gene sequences. Here we demonstrate that this protection is primarily due to the presence of N ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1092-1133
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Engineered Resistance to Tomato Spotted Wilt Virus, a Negative–Strand RNA Virus (1991)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 9 (1991), S. 1363-1367 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] For a growing number of positive–strand RNA viruses, it has been demonstrated that transformation of host plants with the viral coat protein gene confers resistance to the corresponding virus. Thus far, successful transformation strategies to obtain resistance to negative–strand RNA ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1291-1363
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Resistance to tomato spotted wilt virus in transgenic tomato hybrids (1955)
    Springer
    Euphytica 85 (1955), S. 159-168 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; tomato spotted wilt virus ; tospovirus ; transformation ; virus resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tomato spotted wilt virus (TSWV) causes significant economic losses in the commercial culture of tomato (Lycopersicon esculentum Mill.). Culture practices and introgression of natural sources of resistance to TSWV have only been marginally effective in controlling the TSWV disease. Recently however, high levels of protection against TSWV have been obtained by transforming tobacco with a chimaeric gene cassette comprising the TSWV nucleoprotein gene. This report demonstrates the successful application of this newly-created TSWV resistance gene in cultivated tomato. Transformation of an inbred tomato line with the TSWV nucleoprotein gene cassette resulted in high levels of resistance to TSWV that were maintained in hybrids derived from the parental tomato line. Therefore, transformant lines carrying the synthetic TSWV resistance gene make suitable progenitors for TSWV resistance to be incorporated into the breeding programmes of tomato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023944
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Breeding of transgenic orange Petunia hybrida varieties (1955)
    Springer
    Euphytica 85 (1955), S. 403-409 
    Details
    ISSN: 1573-5060
    Keywords: Petunia hybrida ; petunia ; flower ; colour ; DFR ; maize dihydroflavonol-4-reductase ; anthocyanin biosynthesis ; genetic modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Colour is the major contributor to the total ornamental value of a flower. The combination of biochemical knowledge and genetic engineering technology has resulted in the addition of a new colour to the existing colour range of Petunia hybrida. This has been achieved by expression of the maize dihydroflavonol-4-reductase (dfr) gene in a suitable petunia acceptor which leads to the accumulation of pelargonidin-derived pigments in flowers. The resulting flower colour, however, was a pale brick-red, which is commercially unattractive in petunia. Our objective was to produce a product suitable for commercialisation by introducing the dfr gene into our breeding material via normal sexual recombination. Although the initial transformant exhibited many negative characteristics, first analyses indicated that it was feasible to obtain suitable material for creating commercial hybrids. Experimental hybrids based on F4 lines were obtained with improved phenotypic expression of the orange flower colour in combination with a good general performance. In order to assess consumer-related characteristics, selected experimental hybrids were tested under field conditions. All transgenic plants had a normal appearance when compared with non-transgenic control plants. No linkage was observed between the transgenic trait and any negative characteristic. From these studies it can be concluded that through a combination of biochemistry, breeding and genetic engineering, it is possible to generate unique flower colours in a cultivars with commercial potential.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023973
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Coat protein-mediated protection to cucumber mosaic virus infections in cultivated tomato (1996)
    Springer
    Euphytica 88 (1996), S. 139-149 
    Details
    ISSN: 1573-5060
    Keywords: coat protein ; cucumber mosaic virus ; Lycopersicon ; tomato ; transformation ; virus resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cucumber mosaic virus (CMV) infections rank among the most devastating diseases in the commercial culture of tomato (Lycopersicon esculentum Mill.), for which suitable sources of natural resistance are not available. The concept of pathogen-derived resistance, however, offers an alternate approach to combat plant viral diseases by transformation of crops with nucleotide sequences derived from the viral genome. This report demonstrates the successful application of such a pathogen-derived resistance gene comprising the CMV coat protein (CP) gene, to generate protection to CMV infections in cultivated tomato. Transformation of an inbred tomato line with the CMV CP gene isolated from a subgroup I strain, engendered high levels of protection to various CMV strains, including a virulent strain causing lethal necrosis and a typical subgroup II strain. Moreover, when challenged by natural infection through aphid vectors in open field, levels of protection were largely maintained in hemizygous hybrids. In all, these results demonstrate that synthetic resistance genes based on the CMV CP gene make excellent sources of broad spectrum resistance to CMV infections for introgression into tomato breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032445
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Breeding of transgenic orangePetunia hybrida varieties (1995)
    Springer
    Euphytica 84 (1995), S. 175-181 
    Details
    ISSN: 1573-5060
    Keywords: Petunia hybrida ; petunia ; flower ; colour ; DFR ; maize dihydroflavonol-4-reductase ; anthocyanin biosynthesis ; genetic modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Flower colour is the major contributor to the total ornamental value of a flower. The combination of biochemical knowledge and genetic engineering technology has resulted in the addition of a new colour to the existing colour range ofPetunia hybrida. This has been achieved by expression of the maize dihydroflavonol-4-reductase (DFR) gene in a suitable petunia acceptor which leads to the accumulation of pelargonidin-derived pigments in flowers. The resulting flower colour, however, was a pale brick-red, which is commercially unattractive in petunia. Our objective was to produce a product suitable for commercialisation by introducing the DFR gene into our breeding material via normal sexual recombination. Although the initial transformant exhibited many negative characteristics, first analyses indicated that it was feasible to obtain material for creating commercial hybrids. Experimental hybrids based on F4 lines were obtained with improved phenotypical expression of the orange flower colour in combination with a good general performance. In order to assess consumer-related characteristics, selected experimental hybrids were tested under field conditions. All transgenic plants had a normal appearance when compared to non-transgenic control plants. No linkage was observed between the transgenic trait and any negative characteristic. From these studies it can be concluded that through a combination of biochemistry, breeding and genetic engineering it is possible to generate unique flower colours in a cultivar with commercial potential.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01681809
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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