ISSN:
1432-1017
Keywords:
Single-stranded DNA
;
oligonucleotide
;
solution structure
;
NMR
;
nuclear Overhauser effect
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract A 500-MHz 1H-NMR study on the single-stranded DNA undecamer (11-mer) 5′d AAGTGTGATAT is presented. Using a combination of one-dimensional pre-steady-state nuclear Overhauser enhancement (NOE) measurements and two-dimensional homonuclear J-correlated spectroscopy, virtually complete resonance assignments are obtained. The relative magnitudes of the intra- and internucleotide NOEs indicate that the overall structure of the single-stranded 11-mer is a right-handed B-type helix with extensive base stacking. Within this overall structure there is quite a large degree of variability, as exemplified by variations in glycosidic bond and sugar pucker conformations, most likely determined by base sequence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00276624
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