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  • 1
    Electronic Resource
    Electronic Resource
    IgE-mediated allergy to wood dusts probably does not explain the high prevalence of respiratory symptoms among Swedish woodwork teachers (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 50 (1995), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A previous study revealed an increased occurrence of work-related respiratory complaints among Swedish woodwork teachers. For determination of whether an IgE-mediated mechanism was the cause of the symptoms, 127 woodwork teachers and 111 reference subjects (other school personnel) in Stockholm gave serum for analysis of total IgE, Phadiatop, and RAST to extract of sawdust from five commonly used Scandinavian woods (pine, birch, juniper, alder, and linden). The total serum-IgE level was similar in the woodwork teachers (geometric mean 35, range 〈2–1700 kU/l) and the reference group (34, 〈2–860 kU/l), and the frequency of positive Phadiatop was equal (29.9% and 31.5%, respectively). RAST was positive only in three cases, two reference subjects (birch and pine) and one woodwork teacher (alder). Hence, no correlation between exposure and sensitization to wood dust was found, and the results do not support the assumption that the complaints related to exposure to dusts from the examined Scandinavian woods are IgE mediated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1995.tb01199.x
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  • 2
    Electronic Resource
    Electronic Resource
    IgG1 and IgG4 antibody responses to the dust mite Lepidoglyphus destructor in a naturally exposed farming population (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 50 (1995), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The natural humoral immune response to the dust mite Lepidoglyphus destructor was assessed by comparing the IgG1 and IgG4 responses elicited in allergic (n= 44) and healthy (n= 16) individuals in a farming population chronically exposed to this allergen. With the aid of an immunoblotting technique and ELISA, the sera were analyzed for anti-L. destructor antibodies. While the majority of sera from the allergy group displayed several bands for both IgG1 and IgG4, the nonatopic healthy group was negative as analyzed in immunoblotting. When they were analyzed in ELISA, there was a significantly higher response in the allergy group than in the healthy group for IgG4, but not for IgG1. Taken together, these results imply that the immune system of individuals spared from allergic reactions to L. destructor not only lacks IgE antibodies but also seems largely to “ignore” these allergens/antigens despite exposure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1995.tb01181.x
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  • 3
    Electronic Resource
    Electronic Resource
    ELISA method for detection of mite allergens in barn dust: comparison with mite counts (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 51 (1996), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: ELISA (enzyme-linked immunosorbent assay) inhibition with a monoclonal antibody (mAb) (42B6) to Lepidoglyphus destructor was used to detect and quantify the storage-mite allergens in 30 dust samples collected from barns. Regarding the mite fauna, microscopic inspection of the barn dust and mite counts showed that L. destructor infested all 30 barns investigated (range 430–195400 mites/g dust). In 29/30 barns, L. destructor constituted more than 70% of the Astigmata species. Acarus siro was found in 26 samples, the highest value being 16 155 nites/g. No Dermatophagoides species were found. As to mites of the suborder of Prostigmata, species belonging to seven different families were detected. Besides the predominant L. destructor, allergens derived from other storage mites such as Glycyphagus domesticus, A. siro, and Tyrophagus putrescentiue have previously been assessed by this ELISA method. The correlation between number of mites and concentrations of mite antigen as measured by ELISA was assessed by linear regression (r2= 0.83). Thus, inhibition of mAb 42B6 in ELISA would seem to offer a simple and reliable method to detect levels of dust-mite species belonging to the Acaridae and Glycyphagidae families.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1996.tb04602.x
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  • 4
    Electronic Resource
    Electronic Resource
    Occurrence of IgE antibodies against the 39-kDa allergen component of the mite Lepidoglyphus destructor in urban and rural subjects (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 48 (1993), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Several species of storage mite have been shown to cause IgE-mediated sensitization. Monoclonal antibodies (mabs) have been raised against the storage mite Lepidoglyphus destructor, and one mab (42B6) immunoprecipitated a band of 39 kDa of L. destructor. To search for the frequency of IgE antibodies in patient sera against this allergen component, we modified a radioimmunoassay (RIA), 42B6 being used as capturing antibody. Twenty of 43 sera from farmers who were RAST positive to L. destructor (46.5%) were scored as positive in this assay. There was a moderate degree of correlation between positive RAST results to L. destructor and the 39-kDa allergen. Among 14 sera from Stockholm residents positive in RAST to both Dermatophagoides pteronyssinus and L. destructor, six detected the 39-kDa allergen component. However, three sera from urban subjects lacking IgE antibodies against both mite species also had IgE antibodies against the 39-kDa allergen. The present study demonstrates that the 39-kDa allergen of L. destructor is an important allergen, and that rural as well as urban subjects can become sensitized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1993.tb00716.x
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  • 5
    Electronic Resource
    Electronic Resource
    Working with male rodents may increase risk of all_ergy to laboratory animals (2001)
    Copenhagen : Munksgaard International Publishers
    Allergy 56 (2001), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Our aim was to study the risk of laboratory animal all_ergy (LAA) among research staff working in laboratories separate from the animal confinement area. The roles of atopy and exposure intensity in LAA were studied with special regard to exposure to male rodents, who excrete higher levels of urinary all_ergens than female rodents. Methods: Eighty rodent-exposed subjects gave blood samples for the analysis of total IgE, Phadiatop, and specific IgE against rat (RUA) and mouse urinary all_ergens (MUA), and answered questionnaires. Air samples were collected for RUA and MUA aeroall_ergen measurement in both laboratories and animal confinement facilities. Results: Twenty percent of the subjects had IgE 〉0.35 kU/l to RUA and/or MUA, and 32% had experienced animal work-related symptoms, although 90% of aeroall_ergen samples from the research department laboratories were below the detection limit (〈0.26 ng RUA per m3 and 〈0.8 ng MUA per m3). Atopy (positive Phadiatop), total IgE 〉100 kU/l, other all_ergies (especiall_y to other animals), or more than 4 years of exposure significantly increased laboratory animal sensitization and symptoms. Working with mainly male rodents gave odds ratios (95% CI) of 3.8 (0.97–15) for sensitization and 4.4 (1.4–14) for symptoms. Subjects with both exposure to mainly male rodents and atopy or elevated total IgE had a 10-fold higher frequency of sensitization than exposed subjects with neither risk factor. Conclusions: A majority of subjects with a combination of exposure to mainly male rodents and atopy or elevated total IgE developed sensitization to and symptoms from laboratory animals. Current low exposure seems to maintain the presence of specific IgE. Further measures must be undertaken to provide a safe workplace for laboratory animal workers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2001.00899.x
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  • 6
    Electronic Resource
    Electronic Resource
    A new method for collecting airborne allergens (2000)
    Copenhagen : Munksgaard International Publishers
    Allergy 55 (2000), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: xxBackground: Measurement of airborne allergens has hitherto been done with the use of fixed-location pumps or personal air samplers. Our objective was to find out whether ionizers could be good tools for collecting airborne allergens. As a model we have used cat allergen (Fel d 1). We have compared Fel d 1 levels collected by the ionizer at different time periods, as well as comparing Fel d 1 levels obtained with the ionizer with those of low- and high-volume pumps. Methods: Dust samples from floors and air samples collected with ionizers and pumps, obtained in 31 homes with cat, 23 homes without cats, and 28 day-care centres, were analysed for cat allergen content (Fel d 1) by ELISA. Results: Fel d 1 was present in the reservoir in all homes with cats, ranging from 660 to 375000 ng/g (GM 75000) and in the air collected by the ionizer from 2.0 to 204 ng/24 h (GM 19.3). The allergen in homes without cat varied from 〈55 to 1800 ng/g (GM 166). Corresponding levels in air were found in two of these homes (2.3 and 7.3 ng/24 h). There was a correlation between the number of cats and the amount of airborne cat allergen (r: 0.47; P〈0.05). The levels in day-care centres were 〈55 to 3070 ng/g in dust (GM 360) and 〈1.1 to 7.9 ng/24 h in the air (GM 1.6). We obtained a moderately strong correlation between air and dust samples in homes with cats (rs: 0.64; P〈0.001) and in day-care centres (rs: 0.49; P〈0.05). We found that a collection period of 24 h is preferable for the ionizer. The intrahome reliability coefficient was nearly two times higher for the ionizer (r: 0.69) than the pump (r: 0.39). Conclusions: The ionizer seems to be a good tool for monitoring the environment. It is easy to use and silent and does not disturb the airflow in the room.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2000.00652.x
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  • 7
    Electronic Resource
    Electronic Resource
    Exposure to an abundance of cat (Fel d 1) and dog (Can f 1) allergens in Swedish farming households (1999)
    Copenhagen : Blackwell Publishing Ltd
    Allergy 54 (1999), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Earlier studies have shown that farmers are to a low degree sensitized to animal allergens. We have measured the amount of cat (Fel d 1) and dog (Can f 1) in farm households and examined the relationship between exposure and sensitization to cat and dog allergens. Methods: Dust samples from the homes of 403 farmers who had participated in an epidemiologic follow-up study on respiratory symptoms were analyzed for allergen content by two-site ELISA methods. Results: Fel d 1 was detected in 99.5% of the farmers' households ranging from 0.055 to 1455 μg/g dust in mattresses (GM 13.2) and to 3775 μg/g dust in living-room carpets (GM 17.1). Can f 1 was detected in 90.6% of the households from 0.2 to 116 μg/g dust in mattresses (GM 2.0) and to 504 μg/g dust in carpets (GM 4.3). Homes with pets present had the highest levels of the allergens (P〈0.001). A total of 8.4% and 7.4% of the farmers were sensitized to cat and dog, respectively. A significant correlation was noted between exposure to the allergens and specific IgE to cat and dog, respectively (P〈0.001). Sensitization to cat (OR=4.9) and dog (OR=17.8) was significantly associated with asthma. Conclusions: In spite of the abundance of Fel d 1 and Can f 1, farmers are only to a low degree sensitized to cats and dogs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.1999.00855.x
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  • 8
    Electronic Resource
    Electronic Resource
    Detection of at least one high-molecular-mass, IgE-binding component of the dust mite Lepidoglyphus destructor (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 49 (1994), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We studied the allergen composition in an extract of the storage mite Lepidoglyphus destructor. Monoclonal antibodies (mAbs) were raised against L. destructor by a standard hybridoma technique. In the subsequent screening, we concentrated on mAbs fulfilling three criteria. First, in ELISA, mAbs were assessed against a panel of various mite species, and only those reacting exclusively with L. destructor extract were selected for further analyses. Secondly, mAbs were selected in immunoblotting according to whether or not a novel pattern of reactivity emerged in comparison with earlier results. Thirdly, by radioimmunoassay (RIA), we selected mAbs that recognized components which were also recognized by human IgE from sera RAST positive to L. destructor. This yielded an L. destructor-specific mAb (117F9) reacting with two previously unknown components of approximately 79 and 93 kDa, respectively. We also analyzed 80 sera for the presence of IgE binding to these components. These sera were divided into three groups according to their RAST specificity. Eighteen of the 30 sera (60%) that were RAST positive to L. destructor were also positive in RIA. Correlation was moderate between kU/1 for L. destructor and the counts per minute values for the two components in RIA. The group of control sera lacking IgE antibodies against L. destructor displayed no positive results in RIA. However, 4/20 sera RAST positive to Dermatophagoides pteronyssinus but negative to L. destructor were scored as positive in RIA. We conclude that at least one of the high-mol.-mass components of L. destructor causes IgE-mediated sensitization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1994.tb00129.x
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  • 9
    Electronic Resource
    Electronic Resource
    Comparison of clinical significance and allergenic cross-reactivity of storage mites Blomia kulagini and Lepidoglyphus destructor in Sweden and Brazil (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 45 (1990), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Comparison of the clinical significance and allergenic cross-reactivity of Blomia kulagini(B. kulagini) and Lepidoglyphus destructor (L. destructor) was made on sera from Sweden and Brazil using the radio-allergo-sorbent test (RAST) and the RAST inhibition technique. RAST-positive sera were obtained from 53 allergic Swedish farmers and 31 allergic subjects from Brazil who were positive to B. kulagini and/or L. destructor. B. kulagini was shown to be a common cause of sensitization especially in Brazil. There was a fairly high correlation between positive RAST results to L. destructor and B. kulagini based on sera from both Sweden and Brazil. The highest RAST scores were found against L. destructor in Swedish sera and against B. kulagini in Brazilian sera. The RAST inhibition studies showed that the L. destructor extract was able to inhibit the B. kulagini system (a positive RAST to B. kulagini allergen disc) in Swedish but not in Brazilian sera. In contrast, the B. kulagini extract was only able to inhibit the L. destructor system in sera from Brazil and not in sera from Sweden. This study shows that results obtained with RAST inhibition are not entirely dependent on the overall specificity of the IgE antibodies in the patient's sera, since the more subtle specificity of the primarily sensitizing allergen will dominate. Thus, conclusions drawn regarding allergenic cross-reactivity are dependent on the populations tested, and conclusions on the existence or absence of cross-reactivity, e.g. between two species of mites may be contradictory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1990.tb01091.x
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  • 10
    Electronic Resource
    Electronic Resource
    Bronchial provocation studies in farmers with positive RAST to the storage mite Lepidoglyphus destructor (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 43 (1988), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Bronchial provocation studies with L. destructor (L. des.) extract were made on 12 farmers with asthma who were exposed daily to stored hay and grain and had positive radio-allergo-sorbent test (RAST) to L. des. All 12 farmers developed more than a 20 % fall in FEV1 to the extract. None of the symptomatic farmers who were also RAST positive to the L. des. culture medium developed an asthmatic reaction to the medium extract. Neither four healthy farmers nor two patients with asthma caused by D. pteronyssinus (D. pt.) responded to L. des. or the L. des. culture medium. Challenges with D. pt. extract were negative in two symptomatic farmers who were RAST positive to L. des. and negative to D. pt. The present study further supports our previous hypothesis that there is an IgE-mediated immunological mechanism behind L. des. -induced asthma in farmers and that atopic farmers are liable to develop occupational asthma caused by allergy to L. des..
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1988.tb00926.x
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