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  • 1
    Electronic Resource
    Electronic Resource
    Energetics of swimming in Callinectes sapidus and walking in Homarus americanus
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 82 (1985), S. 267-279 
    Details
    ISSN: 0300-9629
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0300-9629(85)90853-9
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  • 2
    Electronic Resource
    Electronic Resource
    Bone Marrow Stromal Cells are Load Responsive In Vitro (1996)
    Springer
    Calcified tissue international 58 (1996), S. 101-108 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Bone marrow — Mechanical load — Differentiation — Prostaglandins —In vitro — Bone cells — Alkaline phosphatase.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Mechanical load-related effects on bone marrow stromal cells in vitro have been investigated. A dose response of a cyclical load of 1 Hz between 350 ustrain and 2500 ustrain applied to 10-day-old cultures resulted in elevated alkaline phosphatase levels and the number of cells expressing this protein after 2 days. No significant changes in the number of cells expressing or the production of collagen type 1 was observed. A critical stage of development of the cultures must be reached before load-related elevation in alkaline phosphatase expression could be measured independent of the stage at which loading was applied. Using a prostaglandin inhibitor at concentrations previously used in vivo, the load response was abolished. We have demonstrated that bone marrow stromal cells are load responsive in culture and have made preliminary studies into determining the involvement of prostaglandins in this process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900018
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Induction of a low voltage-activated, fast-inactivating Ca2+ channel in cultured bone marrow stromal cells by dexamethasone (1994)
    Springer
    Calcified tissue international 54 (1994), S. 125-132 
    Details
    ISSN: 1432-0827
    Keywords: Marrow stromal cells ; Dexamethasone ; Alkaline Phosphatase ; Calcium channels ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The production of biochemical markers associated with the osteoblastic phenotype, and accompanying changes in the expression of voltage-operated Ca2+ channels, have been examined in rat bone marrow stromal cell cultures treated with dexamethasone (10-8 M). Whole cell clamp analysis of voltage-operated Ca2+ channels in control cultures (using Ba2+ as the charge carrier) revealed primarily a high voltage-activated (HVA), slowly inactivating current, which was enhanced two- to threefold by treatment of the cells with Bay K 8644 (300 nM) and inhibited by nifedipine (4 μM). In dexamethasone-treated cultures, the I–V relationship for inward current was shifted to more positive potentials in comparison with control cells. Most cells in these cultures possessed both the HVA current and also a faster inactivating, low-voltage-activated (LVA), nifedepineresistant current. These two currents could be separated both by nifedipine and by the use of steady state inactivation of the LVA current. The two components of the Ba2+ current varied widely in their relative size. The combination of LVA and HVA currents seen in dex-induced stromal cells resembles records of voltage-operated Ca2+ channels from cultures of calvarial osteoblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296063
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  • 4
    Electronic Resource
    Electronic Resource
    Haemolymph oxygen transport and acid-base status in Glyptonotus antarcticus Eights (1997)
    Springer
    Polar biology 18 (1997), S. 10-15 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Haemolymph samples were withdrawn from routinely active male intermoult Glyptonotus held at 0 ± 0.5°C, and analysed for blood-gas and acid-base variables. In both the arterialised (a) and venous (v) haemolymph, over 50% of the oxygen was transported as dissolved oxygen at PaO2 and PvO2 levels of 12.0 ± 1.15 and 7.70 ± 1.89 kPa, respectively. The maximum oxygen-carrying capacity of the haemocyanin (CmaxHcO2) was relatively low at 0.19 ± 0.05 mmol l−1, accompanied by relatively low protein and [Cu2+] levels indicating low circulating haemocyanin concentrations. Arterialised haemolymph had a mean pH of 7.88 ± 0.02(6) at a PCO2 of 0.12 ± 0.01(6) kPa and a bicarbonate level of 12.95 ± 0.80(6) mequiv l−1 with small differences in PCO2 and pH between arterial and venous haemolymph. The non-bicarbonate buffering capacity of Glyptonotus haemolymph was low at −2.0 mequiv l−1 HCO3 − pH unit−1. Haemolymph [l-lactate] and [d-glucose] levels were similar at 〈 1 mmol l−1 in animals held in the laboratory and those sampled in Antarctica. The blood-gas and acid-base status of Glyptonotus haemolymph may be a reflection of the low and stable temperatures experienced by this Antarctic crustacean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003000050153
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  • 5
    Electronic Resource
    Electronic Resource
    The global element method for stationary advective problems (1980)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 15 (1980), S. 167-175 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: We extend the variation principle used in the global element method for self-adjoint elliptic problems1, to problems containing advective terms. Used with the global element formalism, the extended principle yields a uniform framework for treating advective or boundary layer problems, with the attractive feature that the implicit treatment of interface conditions between elements yields an effective decoupling between ‘boundary layer’ and ‘interior’ parts of the solution. As a numerical example, we solve the one-dimensional model problem of Christie and Mitchell3, obtaining high accuracy for Peclet numbers up to 106 with no sign of instability. These results suggest that, given a suitable choice of global elements, the decoupling is very effective in damping the oscillations found in standard finite difference or finite element treatment.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620150202
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    Paper (German National Licenses)
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