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The use of gelatin as a vehicle for drug and peptide delivery (1994)

Silvio, L. ; Courteney-Harris, R. G. ; Downes, S.

Springer

Journal of materials science 5 (1994), S. 819-823

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ISSN: 1573-4838

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine , Technology

Notes: Abstract Gelatin, a naturally occurring polymer, has been investigated as a vehicle for drug delivery in two different delivery systems: microspheres and as a coating on titanium implants. The gelatin was loaded with recombinant human growth hormone (hGH) which was dispersed within the polymer matrix prior to crosslinking; it was then made into microspheres or coated onto the implants. The release of hGH was monitored in vitro using an “in-house” ELISA system. The effects of pH on the swelling kinetics and the physical properties of the loaded gelatin in the microsphere system were studied. In addition, the effect of ultrasound on the microspheres was investigated as a possible method for controlling the rate of release of hGH, it was demonstrated that exposure to ultrasound significantly increased hGH release. Biocompatibility of the gelatin was determined using both primary human (HOB) and rabbit (ROB) osteoblast-like cells in culture.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00213141

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The silver staining of reticular fibers by electrophoresis (1965)

Monroe, Clyde W. ; Harris, R. G.

New York, NY [u.a.] : Wiley-Blackwell

The @Anatomical Record 151 (1965), S. 239-242

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ISSN: 0003-276X

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: In this technique reticulin is stained black with silver by the electrophoretic method. Use Zenker or formalin (10%) fixed tissue, but mordant the latter in Zenker stock for one hour or longer‥ Carry paraffin sections to water; mordant, if necessary; wash well in tap water; transfer to distilled water; place slide in tray containing the ammoniacal silver solution; turn voltage regulator to a potential of 70 v; manipulate the electrodes by passing the negative one back and forth over the section and about 5 mm above it, one minute or less; both electrodes are of 20 gauge copper wire; dip in distilled water; reduce in 5% formalin, three minutes or more; rinse in tap water; to AuCl3, 1-5 minute rinse in tap water; into 5% Na2S2O3. 5 H2O, two minutes; dehydrate, clear, and mount in synthetic resin. This method was used on tissues obtained from human autopsies (liver, lung, scrotum, ovary, esophagus and suprarenal).