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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Surgical and radiologic anatomy 13 (1991), S. 123-128 
    ISSN: 1279-8517
    Schlagwort(e): Sinoatrial node ; Terminal sulcus ; Right auricular crest
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Le nœud sinu-atrial (NSA) a été observé, disséqué et mesuré sur les cœurs de 95 adultes et 30 enfants lors de dissections sous microscope. La majorité des NSA est caractérisée chez l'adulte par une coloration pâle, une consistance ferme, et une situation en regard du lieu de pénétration de l'artère du nœud sinu-atrial, à la partie craniale du sillon terminal. Chez l'enfant, le NSA est toutefois difficile à discerner. Les variations de l'apex de la crête de l'auricule droite et l'incisure à la partie craniale du sillon terminal ont été décrits, et les auteurs suggèrent que le triangle du NSA puisse être considéré comme un important repère pour identifier le NSA. Les caractéristiques de la surface du NSA, ses relations avec le myocarde avoisinant et sa valeur chirurgicale peropératoire sont ensuite discutées.
    Notizen: Summary The sinoatrial nodes (SAN) were observed, dissected, and measured on 95 adults and 30 child hearts under a dissection microscope. The majority of the SANs in adults are characterized by their pale color, firm consistency, and the location in relation to the penetration of the SAN artery, and they can be located in the superior part of the terminal sulcus. The SANs in children, however, are not easily discerned. The variation of the apex of the right auricular crest and the notch in the superior part of the terminal sulcus have been described, and the present authors suggested that the trigone of the SAN could be used as an important landmark to identify the SAN. The surface features on the SAN, its relationship to the surrounding, myocardium and its surgical significance during operation are further discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-4943
    Schlagwort(e): Aminoacylase ; sodium dodecyl sulfate ; inactivation ; conformational change
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract During denaturation by sodium dodecyl sulfate (SDS), aminoacylase shows a rapid decrease in activity with increasing concentration of the detergent to reach complete inactivation at 1.0 mM SDS. The denatured minus native-enzyme difference spectrum showed two negative peaks at 287 and 295 nm. With the increase of concentration of SDS, both negative peaks increased in magnitude to reach maximal values at 5.0 mM SDS. The fluorescence emission intensity of the enzyme decreased, whereas there was no red shift of emission maximum in SDS solutions of increasing concentration. In the SDS concentration regions employed in the present study, no marked changes of secondary structure of the enzyme have been observed by following the changes in far-ultraviolet CD spectra. The inactivation of this enzyme has been followed and compared with the unfolding observed during denaturation in SDS solutions. A marked inactivation is already evident at low SDS concentration before significant conformational changes can be detected by ultraviolet absorbance and fluorescence changes. The inactivation rate constants of free enzyme and substrate-enzyme complex were determined by the kinetics method of the substrate reaction in the presence of inactivator previously described by Tsou [Tsou (1988),Adv. Enzymol. Related Areas Mol. Biol. 61, 381–436]. It was found that substrate protects against inactivation and at the same SDS concentrations, the inactivation rate of the free enzyme is much higher than the unfolding rate. The above results show that the active sites of metal enzyme containing Zn2+ are also situated in a limited and flexible region of the enzyme molecule that is more fragile to denaturants than the protein as a whole.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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