ISSN:
1573-8272
Keywords:
α2C2 adrenergic receptor
;
production
;
purification
;
recombinant
;
Saccharomyces cerevisiae
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The objective is to generate milligram quantities of recombinant human α2C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008150412294
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