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  • 1
    Electronic Resource
    Electronic Resource
    Ein beitrag zur directphotometrie von papierchromatogrammen mit hilfe eines einfachen extinktionenschreibers quantitative bestimug von aminosauren
    Amsterdam : Elsevier
    Journal of Chromatography A 1 (1958), S. 434-445 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(00)93443-5
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Combination of static-field gel electrophoresis and densitometric scanning for the determination of radiation-induced DNA double-strand breaks in CHO cells (1994)
    Springer
    Radiation and environmental biophysics 33 (1994), S. 111-124 
    Details
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract An experimental setup using static-field gel electrophoresis (SFGE) was developed to determine radiation-induced DNA double-strand breaks (DSBs) in CHO-K1 cells after exposure to X-rays or heavy charged particles. The fraction of DNA eluted into the gel matrix depends on the quantity of DSBs introduced. In agreement with a recent report, SFGE and pulsed-field electrophoresis were found to be equally sensitive in DSB detection. With radiolabeled DNA from cell cultures, the absolute amount of DNA migrating out of agarose plugs into the gel was quantified by determining the radioactivity in the gel lane. Alternatively, relative measurements of the amount of DNA released into the gel were achieved with a standardized protocol for both SFGE and a subsequent densitometric scanning of photographic negatives from gels stained with ethidium bromide. After calibration with the radioactive method, the fractions of DNA retained could be calculated directly from the data obtained with the densitometric assay to set up classical dose-effect curves. This procedure was validated for its application with heavy ions using an 500 MeV/u lead beam.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01219335
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Protection of DNA from high LET radiation by two OH radical scavengers, tris (hydroxymethyl) aminomethane and 2-mercaptoethanol (1993)
    Springer
    Radiation and environmental biophysics 32 (1993), S. 21-32 
    Details
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The effect of high linear energy transfer (LET) radiation on DNAin vitro, both in protective and non-protective environments was investigated. Two hydroxyl radical scavengers, tris(hydroxymethyl) aminomethane and 2-mercaptoethanol, were compared for their ability to protect SV40 DNA from radiation damage over a wide LET range. At comparable OH scavenging capacities, significant differences were found between these protective agents, indicating that other, radical scavenger-dependent processes affected the extent to which the DNA was protected. In general, a decrease in single-strand breaks (SSBs) relative to double-strand breaks (DSBs) was observed as LET increased. This effect was more pronounced when a radioprotector was present. Comparison of the relative biological efficiency (RBE) of radiation damage as LET increased showed a peak of DSB production in the mid-LET range. These data agree with measurements made by Christensen et al. (1972). An explanation for this increase in DSB production efficiency has been proposed based on the particle track structure of high-LET radiation.Correspondence to: G. Taucher-Scholz
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213127
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of heavy-ion-induced DNA double-strand breaks using static-field gel electrophoresis (1995)
    Springer
    Radiation and environmental biophysics 34 (1995), S. 101-106 
    Details
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Radiation-induced DNA double-strand breaks (DSBs) were measured in Chinese hamster ovary cells (CHO-K1) using an experimental protocol involving static-field gel electrophoresis following exposure to various accelerated ions. Dose-effect curves were set up, and relative biological efficiencies (RBEs) for DSB induction were determined for different radiation qualities. RBEs around 1 were obtained for low energy deuterons (6–7 keV/µm), while for high energy oxygen ions (20keV/µm) an RBE value slightly greater than 1 was determined. Low energetic oxygen ions (LET=250 keV/µm) were found to show RBEs substantially below unity, and for higher LET particles (≥31 y≥-250 keVµm) RBEs for DSB induction were generally found to be smaller than 1. The data presented here are in line with the generally accepted view that not induced DSBs, but rather misrepaired or unrepaired DNA lesions are related to cellular inactivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275214
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    Paper (German National Licenses)
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