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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Chromatography B: Biomedical Sciences and Applications 565 (1991), S. 1-26 
    ISSN: 0378-4347
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 16 (1995), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Characteristics of the lactobacilli colonizing the various regions of the porcine gastrointestinal tract were investigated. The lumenal contents from the gastric, jejunal, cecal and colonic regions, and biopsies from the gastric non-secreting and secreting regions were homogenized and serial dilutions were spread on Rogosa agar and colonies (50 per region and animal) were randomly sampled. The lactobacillus isolates were grouped according to their protein profiles of lysozyme-treated whole cells. Several different groups of lactobacillus could be detected. Specific groups were associated and often unique for the stomach, jejunal, cecal and colonic regions of the gastrointestinal tract. A few groups were uniformly distributed through the tract. Although published studies of lactobacilli colonizing stomach mucosa have been restricted mainly to the non-secreting region, significant levels were also detected on the secreting stomach tissue. Population densities ranged from 2 × 104 to 2 × 106 CFU per cm2 on both the secretory and non-secretory regions. Of the isolates which colonized the gastric secreting epithelium, 26% formed smooth colonies and 74% formed rough colonies. In contrast, the non-secreting epithelium was mainly colonized by lactobacilli forming smooth colonies (67%). From this study, one can suggest that different lactobacilli are associated with specific regions of the gastrointestinal tract, and that some specific groups appear to be restricted to defined regions.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 153 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Multiplex RAPD-PCR was used to generate unique and identifying DNA profiles for isolates of the genus Lactobacillus. The method that was used was based on the combination of two 10-mer oligonucleotides in a single PCR. The generated RAPD profiles enabled discrimination of all lactobacillus strains that were used in this study. A dendrogram was generated from the RAPD profiles. The results of genetic relatedness obtained from the dendrogram were compared with the results obtained using carbohydrate fermentation profiles. Most of the gastrointestinal isolates studied could not be grouped using carbohydrate fermentation profiles. The RAPD profiles provided sufficient information to prepare a dendrogram of genetic relatedness. The gastrointestinal isolates were clustered together on the dendrogram. Furthermore an isolate originating from the stomach (strain ML004) was closely related to Lactobacillus fermentum. It was concluded that multiplex RAPD-PCR was useful for characterisation and inference of relatedness of Lactobacillus isolates.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 110 (1980), S. 200-204 
    ISSN: 0014-5793
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical chemistry accounts 27 (1972), S. 303-313 
    ISSN: 1432-2234
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Eine semiempirische MO-Methode, die PEEL-Methode, wurde bei der Untersuchung der Elektronenstruktur und der angeregten Zustände von Kupferphthalocyaninen angewendet. Das Absorptionsspektrum des CuPc wird interpretiert. Neue Banden vomn-π *-,d-d- und CT-Typ werden vorhergesagt. Die berechneten Werte der ESR-Parameter stimmen gut mit den experimentellen Werten überein, wodurch gezeigt wird, daß die Methode eine korrekte Elektronenverteilung ergibt.
    Abstract: Résumé Une méthode d'OM semi-empirique, la méthode PEEL, est appliquée à l'étude de la structure électronique et des états excités de la phtalocyanine du cuivre. Le spectre d'absorption de CuPc est discuté et des attributions sont effectuées. De nouvelles bandes de typen-π *,d-d et CT sont prédites. Les valeurs calculées des paramètres ESR sont en bon accord avec les valeurs expérimentales, indiquant que la méthode donne une distribution électronique correcte.
    Notes: Abstract A semi-empirical MO method, the PEEL method, has been applied in an investigation of the electronic structure and excited states of copper phthalocyanine. The absorption spectrum of CuPc is discussed and assignments are made. New bands ofn-π *,d-d and CT types are predicted. The calculated values of ESR parameters accord very well with experimental values, indicating that the method gives a correct electronic distribution.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical chemistry accounts 27 (1972), S. 213-222 
    ISSN: 1432-2234
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Das Elektronensystem der einsamenπ- undσ-Elektronenpaare des Phthalocyaninmoleküls wurde mit Hilfe einer semiempirischen SCF-MO-Methode untersucht. Dabei wurden Elektronen-übergänge vomπ-π *-Typ und vomn-π *-Typ behandelt. Die angeregten Zustände wurden nach der CI-Methode unter Einschluß aller einfach angeregten Zustände berechnet. Die Zuordnung der Ergebnisse zum Elektronenspektrum des Phthalocyanins brachte gute Übereinstimmung mit dem Experiment. Die Lage des niedrigsten elektronisch erlaubtenn-π *-Übergangs wird für das Gebiet der starken Soret-Bande vorhergesagt.
    Abstract: Résumé Les électronsπ et les paires libresσ de la molécule de phthalocyanine ont été étudiés par une méthode SCF MO semi-empirique. On envisage les transitions du typeππ * etn-π *. Les états excités ont été calculés par la méthode de superposition des configurations incluant tous les états monoexcités. Les attributions du spectre électronique de la phthalocyanine sont en bon accord avec l'expérience. La position de la plus basse transition permise du typen-π * est prédite dans la région de la forte bande de Soret.
    Notes: Abstract Theπ andσ lone pair electron system of the phthalocyanine molecule has been studied by a semiempirical SCF-MO method. Electronic transitions of bothπ-π * andn-π * types have been considered. The excited states have been calculated by means of the method of superposition of configurations where all singly excited states are included. Assignments for the electronic spectrum of phthalocyanine could be made in good agreement with experiment. The position of the lowest electronically allowedn-π * transition is predicted to be found in the region of the strong Soret band.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Veterinary research communications 10 (1986), S. 223-228 
    ISSN: 1573-7446
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A new technique, using an intraluminally placed dacron catheter carrying an ultra miniature pressure sensor (MIKRO-TIP, PR-249, Millar Instr., USA) was designed for recording spontaneous intraluminal pressure variations which reflected contractions in the oviductal wall. The catheter was passed subcutaneously from the lumbar back of the animal to the flank where an incision had been made. The distal end of the catheter was passed from a small incision in the uterus tip 2 cm into the isthmus of the oviduct. The catheter's position was secured and control recordings made by connecting the catheter's electrical connector to a polygraph. During the subsequent 24–48 hours successful recordings were made at frequent intervals. Peripheral blood was collected in connection with each recording to monitor ovarian functions.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 33 (1996), S. 31-34 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The adhesion to whole and fractionated porcine gastric mucus of both Lactobacillus fermentum 104-S cells and a saccharide extracted from this strain was investigated. It has been shown previously that this saccharide had affinity for nonsecreting gastric epithelium. The mucus component(s) with affinity the bacterial cells was partly characterized by gel filtration and treatment with protease or metaperiodate. L. fermentum 104-S extracts containing the saccharide were radioactively labeled, fractionated by gel filtration, and tested for affinity for the gastric mucus component showing receptor activity for the whole cells of strain 104-S. The mucus material with affinity for the bacterial cells had a relative molecular weight of 30–70 K. From the results of treatment with protease or metaperiodate, it is proposed that the mucus components(s) that adhered to the whole bacterial cells contained glycoprotein groups. The radioactively labeled saccharide extracted from L. fermentum 104-S cells did not bind to the mucus fraction that had affinity for the whole cells. Conclusively, we suggest that the mechanism by which cells of L. fermentum 104-S adhere to the gastric mucus is different from the mechanism mediating the adhesion of this strain to the nonsecreting gastric epithelium. Cells of L. fermentum 104-S adhere to a glycoproteinaceous mucus component with a relative molecular weight of 30–70 K.
    Type of Medium: Electronic Resource
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