ISSN:
1432-1211
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract To analyze how many D-region-encoded molecules could be detected inH-2 q , we produced a panel of nine monoclonal antibodies from AKR (KkDk) anti-AKR.M (KkDq) immunizations. All of the Dq region antibodies cross-reacted on Dd and/or Ld, and all except one cross-reacted on Db, confirming the previously observed serologic and amino acid sequence homology between theD-region products ofH-2 d ,H-2 b , andH-2 q . All of these monoclonal antibodies precipitated 46 000 dalton molecules from both cell-surface-labeled and biosynthetically labeled BIO.AKM spleen cells, indicating that all were reactive with class I-like molecules. Sequential immunoprecipitation analysis with one of these antibodies, 66-3-5, reveals the presence of a previously unidentified class I-like molecule. Tryptic peptide map analysis reveals that this molecule may be the product of a newly describedH-2D q -region gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00768827
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