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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 5 (1970), S. 70-79 
    ISSN: 1432-0827
    Keywords: Induction ; Transplant ; Chondrogenesis ; Osteogenesis ; Immunosupperession
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Les cellules FL ou WISH provenant de l'amnios humain ont été greffées à des souris d'espèces diverses par voie intramusculaire: 1. Les cellules des deux lignées, greffées à des animaux traités préalablement par la cortisone ou l'ACTH, provoquent la formation du cartilage au voisinage du tissu transplanté. 2. Le méme resultat a été obtenu chez toutes les souris examinées, sans différence selon les espèces. 3. Le cartilage a été remplacé, par de l'os, qui est graduellement résorbé en deux mois. 4. Les cellules FL greffées aux animaux traités par l'hydrocortisone ou l'imurane, ainsi qu'a des animaux irradiés et des souris nouveau-nées, ne provoquent ni la formation de cartilage ni celle d'os.
    Abstract: Zusammenfassung Menschliche amniotische FL- und WISH-Zellen wurden verschiedenen Mäusestämmen intramuskulär implantiert: 1. Implantierung der beiden Zellreihen in Mäuse, die gleichzeitig mit Cortison oder ACTH behandelt wurden, führten zur Knorpelbildung in der Umgebung der implantierten Zellen. 2. Bei keinem der untersuchten Mäusestämme konnten Unterschiede hinsichtlich der Anregung zur Knorpelbildung festgestellt werden. 3. Knorpel wurde durch Knochengewebe ersetzt und letzters innerhalb von 2 Monaten allmählich resorbiert. 4. Implantation der FL-Zellen in die mit Hydrocortison oder Imuran behandelten oder röntgenbestrahlten oder neugeborenen Mäuse führte zu keiner Knorpel- oder Knocheninduktion.
    Notes: Abstract FL or WISH cells, originating from the human amnion, were grafted intramuscularly into various strains of mice. 1. When grafted, cells of both lines evoked cartilage formation in their vicinity, provided that the animals were pretreated with cortisone (5 mg) or ACTH (36 u). 2. No host strain differences were found in respect to the cartilage induction. 3. Cartilage was replaced by bone, tissue, which was gradually resorbed within two months. 4. Grafting the FL cells into animals treated with hydrocortisone or imuran and into newborn or X-irradiated mice failed to induce cartilage or bone tissue.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 192 (1979), S. 148-157 
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Ultrasructure Research 60 (1977), S. 12-20 
    ISSN: 0022-5320
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0533
    Keywords: Key words Glioma ; Elastin ; Elastin binding protein ; Proliferation ; Suramin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have recently shown that glioma cell lines, as well as cells of human malignant gliomas in situ, synthesize tropoelastin. In addition, glioma cells degrade tropoelastin using metalloproteinase(s), and the resulting peptides, incapable of assembling in the extracellular fibers, interact with the 67-kDa cell surface elastin binding protein (EBP), to transduce signals leading to up-regulation of cell proliferation. In this report, we show that exposure to the polysulfonated bis-naphthylurea suramin causes accumulation of physiologically active EBP molecules on the cell surface of a panel of glioma cell lines (U87, MG, U251 MG, U343 MG-A, U373 MG, SF 126, SF188, SF539), which results in an increase of cellular attachment to elastin-coated dishes and in an efficient binding of radiolabeled tropoelastin. Moreover, 100–200 μM suramin stimulates [3H]-thymidine incorporation by those tropoelastin-producing glioma cell lines, but not by A 2058 melanoma cells, which do not produce elastin. Treatment of all glioma cell lines with 100 μM suramin consistently increased expression of cyclin A and its cyclin-dependent kinase, cdk 2, to levels reached following the exposure to exogenous elastin-degradation products (κ-elastin). Our data suggest that a suramin-stimulated accumulation of EBP molecules on the cell surface of glioma cells amplifies the elastin-derived signals, leading to their progression through the cell cycle.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 224 (1989), S. 167-179 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The calcification of cartilage matrix in endochondral bone formation occurs in an extracellular matrix composed of fibrils of type II collagen with which type X collagen is closely associated. Also present within this matrix are the large proteoglycans containing chondroitin sulfate which aggregate with hyaluronic acid. In addition, the matrix contains matrix vesicles containing alkaline phosphatase. There is probably a concentration of calcium as a result of its binding to the many chondroitin sulfate chains. At the time of calcification, these proteoglycans become focally concentrated in sites where mineral is deposited. This would result in an even greater focal concentration of calcium. Release of inorganic phosphate, as a result of the activity of alkaline phosphatase, can lead to the displacement of proteoglycan bound calcium and its precipitation. The C-propeptide of type II collagen becomes concentrated in the mineralizing sites, prior to which it is mainly associated with type II collagen fibrils and is present in dilated cisternae of the enlarged hypertrophic chondrocytes. The synthesis of type II collagen and the C-propeptide, together with alkaline phosphatase, are regulated by the vitamin D metabolites 24,25(OH)2 cholecalciferol and 1,25 (OH)2 cholecalciferol. At the time of calcification, type X collagen remains associated with type II collagen fibrils. It may play a role in preventing the initial calcification of these fibrils focusing mineral formation in focal interfibrillar sites. This process of calcification is clearly very complex, and involves different interacting matrix molecules and is carefully regulated at the cellular level.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 39 (1998), S. 40-51 
    ISSN: 0021-9304
    Keywords: polyethylene particles ; macrophages ; phagocytosis ; cytokines ; histology ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: In this study, an in vitro model has been developed to examine the interactions of macrophages with ultrahigh molecular-weight polyethylene (UHMWPE) and high-density polyethylene (HDPE) particles. Polyethylene particles are the major constituent of the material debris formed as a result of orthopedic implant wear. However, the study of polyethylene particle interactions with cells has been limited. UHMWPE (18-20 μm) and HDPE (4-10 μm) were suspended in soluble collagen type I and subsequently solidified on glass coverslips. The particle chemistry was characterized by Fourier transform infra-red spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). Mouse cell line macrophages (IC-21) were established on the collagen-particle substrata and maintained for up to 24 h. The response of the cells to the particles was examined by light and transmission electron microscopy (LM and TEM), as well as by scanning electron microscopy (SEM), and compared to cells on control collagen surfaces without particles. Histological analysis of the samples revealed that the macrophages surrounded larger particles (18-20 μm) and the cells appeared to be attached to the surface of the particles, and the smaller particles (4-10 μm) had been phagocytosed within 2 h. Inflammatory cytokines (TNF-α, IL-1α, IL-1β, and IL-6), lysosomal enzymes (β-galactosidase and hexosaminidase), and prostaglandin E2 were released into the medium, and IL-1α, IL-1β, PGE2, β-galactosidase, and hexosaminidase levels were significantly increased over collagen control values. The results demonstrate active phagochemotaxis by macrophages for wear particulates and validate this model as a means of studying the specific in vitro interactions of polyethylene with cells. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 40-51, 1998.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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