Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Purification and sequencing of the active site tryptic peptide from penicillin-binding protein 1b of Escherichia coli (1985)
    s.l. : American Chemical Society
    Biochemistry 24 (1985), S. 3448-3453 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00335a009
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Acriflavine as an Effective Agent for eliminating F -factor in Escherichia coli K -12 (1957)
    [s.l.] : Nature Publishing Group
    Nature 180 (1957), S. 655-656 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] An overnight culture of F -f cells is inoculated into a nutrient broth containing 'Kyokuto' broth 10 gm., 'Kyokuto' peptone 10 gm., sodium chloride 2 gm., and distilled water 1 litre or 'Difco' broth 10 gm., 'Difeo' peptone 10 gm., and distilled water 1 litre, with a sublethal concentration of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/180655a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Effects of radioactive decay on the autonomous replicatin of anEscherichia coli episome (1964)
    Springer
    Molecular genetics and genomics 95 (1964), S. 184-194 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary TheE. coli episomeF 13 loses its capacity for autonomous replication when the integrity of the molecule is impaired by the decay of32P atoms incorporated into its polynucleotide chains. Upon further division of the host bacteria, the distal fragments near the episomal terminus are eliminated, whereas, the proximal fragments near the episomal origin may survive as heterogenotes attached to the host chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00894919
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Replication origin of the Escherichia coli K-12 chromosome: The size and structure of the minimum DNA segment carrying the information for autonomous replication (1980)
    Springer
    Molecular genetics and genomics 178 (1980), S. 9-20 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA fragment containing the replication origin of the Escherichia coli K-12 chromosome was inserted in two orientations at either the BamHI or SalI site of pBR322 DNA. All the resulting hybrid plasmids were found to replicate in both polA and polA + cells, whereas pBR322 replicates only in polA + cells. This characteristic provided a method for assaying the autonomously replicating ability (Ori function) of the E. coli origin. In order to define the minimum DNA region (ori) that determines Ori function, deletions of various sizes were introduced from either side of the ori-containing segment in the hybrid plasmids by in vitro techniques, and the correlation between the Ori phenotype and nucleotide sequence of the deletion derivatives was analyzed. It was found that the left end of ori is between positions 23 and 35, and the right end is either position 266 or 267 in our nucleotide coordinate (Sugimoto et al., 1979). Therefore, ori is present within a region of minimum 232 base pairs and maximum 245 base pairs in length. The Ori+ and Ori- phenotypes were clearly resolved at both sides of these boundaries by the above assay procedure. To obtain information about the effect of mutations in the internal region of the defined ori stretch, short sequences were inserted or deleted in vitro in the vicinity of several restriction sites within ori on the hybrid plasmids. Most of these plasmids carrying modified sequences showed Ori- phenotype, suggesting that most parts of the ori stretch play important roles in Ori function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267207
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Overlapping of the coding regions for α and γ components of penicillin-binding protein 1 b in Escherichia coli (1984)
    Springer
    Molecular genetics and genomics 196 (1984), S. 449-457 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mode of biosynthesis of penicillin-binding protein(PBP)-1 b in Escherichia coli was investigated by use of the plasmid carrying the ponB(PBP-1 b) gene region. Analyses of the products synthesized in minicells and in vitro showed that PBP-1 b was synthesized as two molecular species corresponding to the α and γ components of PBP-1 b. The coding regions for the α and γ components were located within the ca. 3.7 kb MluI-HincII fragment and transcribed in the direction from the HincII to the MluI site. The capacity for producing the α component was abolished by a deletion extending to the MluI site ca. 0.7 kb inward from the HincII end of the ca. 3.7 kb fragment; the remaining 3.0 kb region with the MluI site at both ends directed the production of the γ component alone. The production of the γ component was enough to correct all the known defects caused by a ponB mutation. In addition to these results, the analyses for cross-reacting materials produced in correspondence to the various deletions indicated that the coding regions for the α and γ components overlapped and that the N-terminal portion was responsible for the difference between the two components. The distal region about 0.7 kb long inward from the MluI end of the MluI-HincII fragment was dispensable for producing the functional PBP-1 b, although the PBP-1 b produced was curtailed. By a larger distal deletion reaching almost to the middle of the MluI-HincII fragment, the polypeptide produced for PBP-1 b lost the ability to bind penicillin and still retained a low but significant activity for glycan synthesis. We suggest, therefore, that the polypeptide portion required for transglycosylase activity resides on the N-terminal half of PBP-1 b, followed by the middle portion necessary for penicillin-binding and the C-terminal part dispensable for the function of PBP-1 b.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436192
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Mutants of Escherichia coli defective in penicillin-insensitive murein DD-endopeptidase (1983)
    Springer
    Molecular genetics and genomics 189 (1983), S. 215-221 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two mutants of Escherichia coli that are deficient in the penicillin-insensitive DD-endopeptidase have been isolated. The strain JE10874 (mepA) has about 10%–20% of the residual activity and another strain, JE10368 (mepB) has 40%–50% of the activity found in the wild-type, parental strain, PA3092. The penicillin-insensitive endopeptidase is a periplasmic enzyme. Genetic mapping studies show that the mutation mepA is located close to aroC (50 min) and the other mutation, mepB, is very close to malE (91 min) on the chromosome. These mutants grow normally under a wide range of growth conditions; other phenotypic properties of the mutants are very similar to those of the parent strain. A double mutant (mepA mepB), and a triple mutant (mepA mepB dacB), deficient in both penicillin-insensitive and penicillin-sensitive endopeptidases, were constructed. Again, these mutants grew normally. We conclude that either the very low level of residual enzyme activity in the mutants is enough for their survival or that the penicillin-insensitive endopeptidase is not essential for survival under laboratory conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337807
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Dispensability of either penicillin-binding protein -1a or -1b involved in the essential process for cell elongation in Escherichia coli (1985)
    Springer
    Molecular genetics and genomics 200 (1985), S. 272-277 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A strain of Escherichia coli lacking the entire ponB gene and a strain lacking the proximal part of the ponA gene were constructed by substitution with a drug resistance gene. These strains lost either penicillin-binding protein(PBP) -1b or -1a totally and their growth was apparently normal at 30°C and 42°C except that growth of the ponB deletion strain was poor on a nutrient agar plate containing no NaCl at 30°C as well as at 42°C. Transductional experiments to introduce the ponB deletion into the ponA deletion strain, and vice versa, showed that the ponA ponB double deletion was lethal unless the deletion was functionally compensated, e.g., by the presence of a plasmid carrying either gene. Thus, either PBP-1b (ponB) or PBP-1a (ponA), but not both, is dispensable for cell viability, at least under ordinary culture conditions. Transductional experiments also suggested that the γ component of PBP-1b or the PBP-1b lacking the C-terminal portion encoded in the distal region to the SphI site on the ponB was sufficient for supporting growth of the E. coli cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425435
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Characterization of the dnaA gene carried by lambda transducing phage (1980)
    Springer
    Molecular genetics and genomics 180 (1980), S. 235-247 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Specialized transducing phages λdnaA were obtained by inducing lysogens in which λtna was integrated at the tnaA region of the Escherichia coli chromosome; the tnA region is located in the vicinity of the dnaA gene. The dnaA - deletion derivatives of λdnaA were isolated from the lysate of λdnaA grown on bacteria carrying a transposon Tn3. The structures of various transducing phages thus obtained were determined by heteroduplex DNA mapping. From these results, the transducing fragment of 13.8-kb-long was divided into nine domains. Upon infection of UV-irradiated cells with the phage, production of polypeptides of 49 kD and 42 kD was specifically associated with infections by the dnaA and recF transducing phages. Polypeptides of 49 kD and 42 kD appeared to be coded for by dnaA and recF genes, respectively. The dnaA gene was assigned to the region of 2.8-kb-long which extends by 2.4 kb in the counterclockwise direction on the E. coli genetic map and 0.4 kb in the opposite direction, as measured from the nearest HindIII site close to the tnaA gene. The recF gene was also discovered to lie very close to dnaA in the order of tnaA-dnaA-recF. Merogenotes heterozygous for the dnaA gene were constructed by introducing F′100-12 carrying λdnaA into the recipients with different mutations at or near dnaA. For combinations, F′(λdnaA +)/dnaA46 and F′(λdna +)/dna-83, dnaA + was trans-dominant, whereas the dnaA + was recessive for F′(λdnaA +)/dna-5. For F′(λdnaA +)/dna-167, the result of the transdominance test was affected by the growth media employed; dnaA + was dominant on a λ-broth plate, and dna-167 was dominant on an M9-minimal plate. Thus, transdominance of dnaA + in heterozygotes is affected by difference in mutations and growth media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425835
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Suppressor genes of a dnaA temperature sensitive mutation in Escherichia coli (1982)
    Springer
    Molecular genetics and genomics 187 (1982), S. 67-71 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recombinant plasmids were constructed from EcoRI digests of Escherichia coli chromosomal DNA and pMB9 DNA by selecting for suppression of a dnaA−T46 temperature-sensitive mutation. Two types of plasmid capable of suppressing the dnaA mutation were isolated. They did not carry any genetic markers around dnaA and physical mapping with various restriction enzymes showed that neither of the plasmids contained the dnaA gene. One plasmid, pYT47, was characterized further and the protein responsible for the suppression was identified by two-dimensional gel electrophoresis. The molecular weight of the suppressor protein was about 68 Kdal and thus is clearly different from the dnaA gene product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384385
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli (1978)
    Springer
    Molecular genetics and genomics 165 (1978), S. 15-20 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Temperature-sensitive mutants of an Escherichia coli K-12 strain PA3092 have been isolated following mutagenesis with nitrosoguanidine, and their ribosomal proteins analyzed by two-dimensional gel electrophoresis This method was found to be very efficient in obtaining mutants with various structural alterations in ribosomal proteins. Thus a total of some 160 mutants with alterations in 41 different ribosomal proteins have so far been isolated. By characterizing these mutants, we could isolate, not only those mutants with alterations in the structural genes for various ribosomal proteins, but also those with impairments in the modification of proteins S5, S18 and L12. Furthermore, a mutant has been obtained which apparently lacks the protein S20 (L26) with a concomitant reduction to a great extent of the polypeptide synthetic activity of the small subunit. The usefulness of these mutants in establishing the genetic architecture of the genes coding for the ribosomal proteins and their modifiers is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270371
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...