ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

AVEC-DIC and electron microscopic analyses of axonally transported particles in cold-blocked squid giant axons (1985)

Fahim, Mohamed A. ; Lasek, Raymond J. ; Brady, Scott T. ; [et al.]

Springer

Journal of neurocytology 14 (1985), S. 689-704

add to mindlist on the mindlist

Details

ISSN: 1573-7381

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Anterogradely and retrogradely transported membranous organelles were analysed separately by focally cooling axons (cold-blocking) for 2–4 h. Video-enhanced differential interference contrast light microscopy (AVEC-DIC) and dark field light microscopy showed that particles accumulated in large numbers on both the anterograde and the retrograde sides of the cold-block and that the accumulated particles resumed their transport when the preparation was rewarmed to 18 °C. The particles accumulated in files on both sides of the cold-block suggesting that particles move along linear pathways in the axoplasm. Comparisons of the results obtained by AVEC-DIC light microscopy with those obtained by electron microscopy indicate that the AVEC-DIC method is capable of detecting all of the different types of rapidly transported membranous organelles, including the smallest (35–80 nm) vesicles that move anterogradely. Electron microscopic analyses of the transported particles demonstrate that the anterogradely transported organelles are structurally distinct from those that are transported retrogradely. The anterogradely transported particles consisted of normal mitochondria and small (35–80 nm) tubulovesicular profiles. By contrast, the retrogradely transported particles were 150 nm or larger and they often contained complex membranous inclusions. The largest retrogradely transported particles appeared to be degenerating mitochondria. The results are consistent with the hypothesis that the direction of organelle movement is related to the physiological state of the organelle. That is, organelles containing newly synthesized membrane components move primarily anterogradely and organelles that contain transformed and degraded membrane components move retrogradely.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01170822

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Direct visualization of particle velocity distribution by pseudostereoscopic viewing of time-lapsed sequential images: Application to fast axonal transport (1984)

Hodge, Alan J. ; Adelman, William J.

New York, NY : Wiley-Blackwell

Cell Motility and the Cytoskeleton 4 (1984), S. 231-239

add to mindlist on the mindlist

Details

ISSN: 0886-1544

Keywords: pseudostereoscopy ; particle speed distribution ; velocity distribution ; fast axonal transport ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: We describe a simple method for direct visualization of the velocity distribution of particles moving against an immobile background. The technique involves pseudostereoscopic viewing of image pairs separated by an appropriate time interval in a sequential recording of the subject. Under these conditions, the positive or negative parallax arising from particle motion results in the binocular image of a particle being perceived as raised or lowered relative to an immobile background plane depending on its direction of movement, and with the degree of perceived elevation being proportional to its speed. In effect, the binocular optic axis becomes a velocity (speed) axis under these conditions. The technique is illustrated with examples of image pair sequences showing fast axonal transport in lobster and squid axons using video-enhanced differential interference contrast microscopy. However, the pseudostereoscopic method is quite generally applicable to both microscopic and macroscopic time-dependent phenomena. Particle speeds can be quantitated using standard procedures for measuring frame-to-frame particle displacements, or alternatively, by determination of parallax using stereogrammatic methods. It should be also readily adaptable for on-line monitoring of particle velocity distribution, particularly in video systems where frame buffers can be utilized to extract and present serial image pairs having any desired time separation from video-taped sequences.

Additional Material: 6 Ill.