ISSN:
1432-1130
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A method for the determination of hydrogen peroxide and several organic peroxides by high-performance liquid chromatography with post-column UV irradiation, derivatization and fluorescence detection is described. By means of post-column UV irradiation in the presence of water organic peroxides are converted into hydrogen peroxide and organic hydroperoxides, which react rapidly with the post-column derivatization agent p-hydroxyphenylacetic acid (PHPAA) under catalysis of horseradish peroxidase to yield the fluorescent PHPAA dimer that is detected at excitation and emission wavelengths of 285 and 400 nm, respectively. The detection limit for hydrogen peroxide is 14 ng/mL, for organic peroxides between 34 ng/mL and 5 μg/mL. No interference by other compounds was observed when their concentrations were below 10 mg/mL except ethers and phenols.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002160050847
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