ISSN:
1662-9752
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The development of tissue engineering provides a novel approach to restore bodilyfunctions by seeding cells onto various scaffolds. Although chitosan is a non-toxic biomaterial, itscytocompatibility still needs to be improved. In this study, gamma-poly(glutamic acid) (γ-PGA) wasblended with chitosan to prepare both dense and porous γ-PGA/chitosan composite scaffolds usingthe freeze-gelation method. This method saves time and energy, and there is less residual solvent.SEM micrographs demonstrated that an interconnected porous structure with a pore size of 30-100micrometer was present in the scaffolds. The hydrophilicity of the scaffolds was significantlyimproved by γ-PGA. Further, the tensile strength of the porous γ-PGA-modified chitosan scaffoldswas about 50% higher than that of the unmodified chitosan scaffolds. The number of osteosarcomacells cultured on the γ-PGA-modified scaffolds was about double that on the unmodified chitosanscaffolds on day 7. Thus, the γ-PGA/chitosan composite scaffolds, due to their better hydrophilicity,cytocompatibility, and mechanical strength, are very promising biomaterials for tissue engineeringapplications. We further demonstrated the use of glutamic acid to enhance the tensile strength ofchitosan-based composite porous scaffolds. The tensile strength of the chitosan/collagen compositescaffolds was increased by more than 2 times with the addition of glutamic acids as cross-linkingbridges. We found that the hepatocytes attached and proliferated well on these composite scaffolds,demonstrating that the glutamic acid modified-chitosan composite scaffolds are also potential tissueengineering biomaterials
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/02/14/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.567.pdf
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