ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
This study is to investigate the effect of PAM on protein adsorption and osteoblastic cellsadhesion to HA bioceramics. PAM was immobilized on the surface of HA bioceramics for bonescaffold by chelating. The outermost layer of the specimens was analyzed by XPS and FT-IR. Theprotein adsorption test was performed using the 10% bovine calf serum absorbed on the specimens invitro. The osteoblastic cells were inoculated and cultured on the scaffolds. SEM, MTT test and ALPactivity test evaluated the cell attachment, proliferation and activity on the scaffolds. Characteristicpeaks in XPS and FT-IR spectra indicated PAM being immobilized on the surface of the bioceramics.PAGE and 2-D DIGE results indicated that HA absorbed more acidic proteins, while PAM-HAabsorbed more basic and neutral proteins.The cell culture test indicated that the cells activelyproliferated on the scaffolds. There was no significant difference between the ALP activity of the cellscultured for 1d, 3d, 5d and 7d on PAM-HA and that of the controls. PAM had no obvious effect on thecytocompatibility of HA, and PAM-HA bioceramics could be used as bone scaffold with potentialability to improve osteogenesis
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.885.pdf
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