ISSN:
1432-136X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary 1. Resting blood metabolite concentrations were determined at 19 different times of the daily cycle (15°C, LD12:12). Blood glucose values for 151 frogs determined over a 6-month period yielded a mean level of 24.5±3.0 mg·100 ml−1 blood (mg%). Blood lactate values for 132 animals yielded a mean level of 31.0±12.3 mg%. 2. Blood glucose concentrations over a 24-hour period showed no diel cycle. Blood lactate values over a 24-hour period were much more erratic, but with a distinct peak one h after the onset of scotophase (1900 hrs CST), suggestive of a diurnal cycle. 3. No correlations were found between normal blood glucose and lactate concentrations and weight, sex, or season. 4. Faradic stimulation of 15- and 30 min duration were equally efficient in eliciting maximal lactate concentrations. A 2-min period of shocking was significantly less effective. 5. Stressed (i.e., due to strenuous exercise) blood metabolite concentrations forR. pipiens were determined 0.0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 8.5 hrs following a 30-min period of Faradic stimulation from 1200 to 1230 hrs CST. Blood glucose levels increased from 20.9 to 57.2 mg% and blood lactate values increased from 18.6 to 99.5 mg% during a period of two hrs following stimulation; normal levels were again attained 4.0 hrs after the end of stimulation. 6. Resting liver, muscle, and total body lactate concentrations were 20.9±3.5, 76.8±17.5, and 44.7±12.5 mg 100 g−1 tissue wt. (mg%), respectively. Stressed tissue lactate levels were determined 0.0, 1.0, 3.0, 4.0, and 7.5 hrs following the Faradic stimulation period. Immediately following the end of stimulation, liver, muscle, and total body lactate concentrations increased by factors of 4.6 (from 20.9 to 95.6 mg%), 3.2 (from 76.8 to 244.0 mg%), and 4.0 (from 44.7 to 180.8 mg%), respectively. After one h of recovery, all three lactate concentrations were reduced abruptly to approximately one-half the peak value and were maintained for several hrs before return to normal values after 7.5 hrs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00710369
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