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  • 1
    Electronic Resource
    Electronic Resource
    InducciÓn Experimental á Malformaciones por Instilacion de Etanol en Huevos Embrionados de Polio (Gallus gallus) (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 24 (1995), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The teratogenic effect of alcohol on chick embryos has been confirmed by many investigators. However, how this occurs is unknown.The aim of this study was to establish a teratogenic pattern of alcohol effects, on the first stages of development in avians.Fertilized eggs were infused through the air space of the shell on day 0, with ethanol in concentrations of 20%, 40% and 60%. The control group was infused with 0.1 ml of NaCl at 0.9%. At a second stage, the eggs were treated on the 4th day of incubation, using the same method. In both groups the eggs were removed on the 11th day of incubation.The teratological manifestations that appeared more frequently were evisceration, haemorrhagic embryos, oedema, cranial deformities, lack of eyes, and umbilical hernia, showing every embryo a clear reduction in size and body weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1995.tb00038.x
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  • 2
    Electronic Resource
    Electronic Resource
    Hatching Gland Cells of Trout Embryos as the Target of Positional Information (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 25 (1996), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: HGCs were found in the head epidermis, yolk sac and pharynx epithelium of trout embryos. These cells usually appear in clusters, closely related positionally to neighbouring cells. The differentiation and specialization of HGCs seem to be mainly dependent on cell-cell interactions, which provides, in part, the positional information necessary for the cells to differentiate and localize in the appropriate place. The final secretory process is the result of a sequence of events by which the maturation of enzymatic granules occurs. The electron-density of the granules varies according to the proximity of the secretory stage. Exocytosis of the secretory granules were observed. After secretion, each HGC undergoes cellular death by apoptosis (programmed cell death).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1996.tb00096.x
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  • 3
    Electronic Resource
    Electronic Resource
    Abstract (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 13 (1984), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In a study of the development of two oviparous species (domestic chicken and Japanese quail) and one ovoviviparous species (a common lizard), the relationship to alterations in egg size and weight during incubation was examined. It was observed, as reported by others that the avian egg diminishes in size during incubation, but that the lizard egg increases in size and weight. This investigation examines these relationships and suggests that the increase in the size and weight of the lizard egg is a result of an increase of liquid content. The mechanism is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1984.tb00388.x
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  • 4
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    Unknown
    Consejo pontificio para los laicos (1990)
    Pamplona : Periodicals Archive Online (PAO)
    Ius canonicum. 30:60 (1990) 493-510 
    Details
    ISSN: 0021-325X
    Topics: Law , Theology and Religious Studies
    Notes: ESTUDIOS SOBRE LA CONSTITUCIÓN
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:p022-1990-030-60-000005
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro differentiation of tooth buds from embryos and adult lizards (L. gravenhorsti): An ultrastructural comparison (1980)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 165 (1980), S. 225-236 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It is well established that the capacity for teeth to differentiate “in vitro” depends upon: (a) the age of the embryonic rudiments at the time of excision and (b) the number of cells within each tissue type which are capable of differentiating into organ culture. This paper studies ultrastructural aspects of tooth buds grown in vitro from lizard embryos and compares these characteristics with those observed in dental germs grown in situ in older lizard embryos. Moreover, we report the self-differentiation in vitro dental tissues from adult lizard and compare this phenomenon with the main features of a morphogenetic field. Our results suggest that approximately in the first third of gestation in L. gravenhorsti the dental buds has already acquired the capacity for self-differentiation in vitro. The ultrastuctural observations show that there are no significant differences between odontoblasts and ameloblasts in situ and in vitro. The tooth from “adult lizards,” isolated by combined microsurgical and enzymatic procedure and cultured in semisolid-liquid medium were also able to differentiate teeth. This phenomenon implies that self-differentiation is not rigidly determined, and that in these animals the tooth tissues represents a continuous morphogenetic field throughout the animal's life. This property is intrinsic, resides in the isolated tooth tissues, and is relatively independent of external factors. In addition, these studies indicate that the chick chorio-allantoic membrane and the semisolid-liquid culture medium supply the majority of the factors required for development of these tissues.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051650302
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  • 6
    Electronic Resource
    Electronic Resource
    Odontogenesis and amelogenesis in interacting lizard - Quail tissue combinations (1986)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 189 (1986), S. 121-129 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this study we examined the possible inductive role of the dental papilla from polyphyodont lizard tooth germs. Flank skin sheets of quail ectoderm enzymatically separated from dermal tissue were recombined with lizard tooth papillae and placed on semisolid medium and cultured for 2 days. Subsequently, the recombinants were removed and placed on the chorioallantoic membrane of chick hosts and incubated for 6 days. After this period of 8 days in explant, control tissues differentiated according to their own phenotypes. Lizard dental papilla alone differentiated as fibroblasts. Quail flank skin ectoderm differentiated into epithelial sheets. Intact lizard tooth buds developed into teeth with dentine and incipient enamel. In the best experimental recombinants, advanced and relatively well-constructed teeth were observed, with clear indications of hard tissue deposition in association with quail epithelium. The results show that mesenchyme of the adult lizard dental papilla and embryonic quail ectoderm of heterotopic origin are capable of carrying out the complex sequence of morphogenetic interactions involved in normal odontogenesis.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051890203
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  • 7
    Electronic Resource
    Electronic Resource
    Comparative analysis of the development of the lizard, Liolaemus tenuis tenuis. II. A series of normal postlaying stages in embryonic development (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 169 (1981), S. 337-349 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this work, we have completed a study of the development of the ovoviviparous lizard Liolaemus tenuis tenuis. Ovoviviparity in this lizard is a condition in which eggs are retained within the reproductive duct for about 60 days. During this period the phases of segmentation, gastrulation, neurulation, presomitic, and somitic embryos transpire. During the months of December and January the eggs are laid, and at this time the embryos are comparable to stage 27 Liolaemus gravenhorsti lizard embryos, or to stage 29 Calotes versicolor lizard embryos. Differentiation of the facial region occurs between Days 12 and 42 after egg laying. Limbs develop rapidly between the 8th and 23rd days. By 53 days the appendicular skeleton is completely formed. After 36 days the mesonephros begins to degenerate, and its function is gradually taken over by the developing metanephros. Newborn lizards do not possess an egg caruncle. During the period up to hatching, there is a great increase of liquid within the egg, presumably amniotic fluid. Cracks develop in the leathery shell shortly before hatching and are, perhaps, the first sign of the onset of hatching. Increase of liquid in the egg during postlaying development accounts for its increase in weight and change in shape. Weight of the embryo at hatching does not exceed 32% of the total weight of the egg.
    Additional Material: 27 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051690307
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructural aspects of dental tissues and their behavior in xenoplastic association (lizard quail) (1983)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 176 (1983), S. 341-350 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ultrastructural characteristics of tooth buds of the polyphyodont adult lizards Liolaemus tenuis and Liolaemus gravenhorsti have been elucidated. Xenoplastic combinations of lizard whole tooth buds and neural crest cells from embryos of the quail Coturnix coturnix japónica have been cultured in vitro. Mesenchymal cells (preodontoblasts) of lizard teeth early develop filopodia that contact the basal lamina. Fragments of quail neural crest isolated by dissection were recombined with isolated lizard tooth buds and cultured for 84 hours in dishes kept in an incubator at 37.8°C in air. Some identifiable quail cells in these recombinants developed a cytoplasmic extension like that of an odontoblastic process. These results suggest that lizard tooth rudiments already determined for tooth development produce some non-species specific transmissible constituents which are capable of inducing quail cranial neural crest cells to express certain dental characteristics (odontoblastogenesis) not expressed in their normal development in vivo.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051760307
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