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  • 1
    Electronic Resource
    Electronic Resource
    Chromosome alterations in tissue culture cells ofAllium fistulosum (1995)
    Springer
    Genetica 96 (1995), S. 191-198 
    Details
    ISSN: 1573-6857
    Keywords: Allium ; mutations ; heterochromatin ; telomeres ; callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterochromatin behaviour and structural alterations in chromosomes of cells derived from callus culture ofAllium fistulosum have been studied. The diploid chromosome complement ofAllium fistulosum consists of 16 chromosomes with significant amount of heterochromatin mainly of telomeric nature. In eight collections of callus cells analysed, a high rate of numerical and structural chromosome abnormalities was observed. After 12 months in culture about 20% of metaphase chromosomes possessed distinct signs of mutational events. C-banded preparations revealed that many structural alterations involved regions of heterochromatin. Interchromosomal connections and chromatid fusions occurred at telomeric heterochromatin segments. Also formation of the end-to-end associations and polycentric chromosomes often took place without visible loss of telomeric heterochromatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01439572
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Megachromosomes in tissue culture ofAllium (1993)
    Springer
    Genetica 90 (1993), S. 35-40 
    Details
    ISSN: 1573-6857
    Keywords: Allium ; chromosomes ; megachromosomes ; tissue-culture ; callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chromosome studies have been conducted on the long-term callus cultures of threeAllium species:A. porrum (2n=32),A. tuberosum (2n=32) andA. fistulosum (2n=16). In cultures ofA. fistulosum several interesting cytological abnormalities were observed. They included direct elimination of chromatin from nuclei, multiple chromosome fusions and formation of polycentric and megachromosomes. The rate of abnormalities increased with the time of culture. Allium fistulosum may provide an excellent model system to analyse cytogenetic and molecular aspects of callus-induced genomic changes and, thus, somaclonal variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01435176
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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