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  • 1
    Electronic Resource
    Electronic Resource
    Coexpression of Several Types of Metabotropic Nucleotide Receptors in Single Cerebellar Astrocytes (2000)
    Oxford UK : Blackwell Science Ltd.
    Journal of neurochemistry 75 (2000), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We have examined the expression of mRNA for several P2Y nucleotide receptors by northern blot analysis in purified type 1 cerebellar astrocyte cultures. These results suggest that different P2Y subtypes could be responsible for ATP metabotropic calcium responses in single type 1 astrocytes. To identify these subtypes we have studied the pharmacological profile of ATP calcium responses using fura-2 microfluorimetry. All tested astrocytes responded to ATP and UTP stimulations evoking similar calcium transients. Most astrocytes also responded to 2-methylthioATP and ADP challenges. The agonist potency order was 2-methylthioATP 〉 ADP 〉 ATP = UTP. Cross-desensitization experiments carried out with ATP, UTP, and 2-methylthioATP showed that 2-methylthioATP and UTP interact with different receptors, P2Y1 and P2Y2 or P2Y4. In a subpopulation of type 1 astrocytes, ATP prestimulation did not block UTP responses, and UDP elicited clear intracellular Ca2+ concentration responses at very low concentrations. 2-MethylthioATP and UTP calcium responses exhibited different sensitivity to pertussis toxin and different inhibition patterns in response to P2 antagonists. The P2Y1-specific antagonist N6-methyl-2′-deoxyadenosine 3′,5′-bisphosphate (MRS 2179) specifically blocked the 2-methylthio-ATP responses. We can conclude that all single astrocytes coexpressed at least two types of P2Y metabotropic receptors: P2Y1 and either P2Y2 or P2Y4 receptors. Moreover, 30-40% of astrocytes also coexpressed specific pyrimidine receptors of the P2Y6 subtype, highly selective for UDP coupled to pertussis-toxin insensitive G protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2000.0752071.x
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  • 2
    Electronic Resource
    Electronic Resource
    Alkaline Activation of Fly Ashes: NMR Study of the Reaction Products (2004)
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 87 (2004), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Reaction products formed during alkaline activation, with 8M NaOH solutions, of fly ashes have been characterized with 29Si and 27Al magic-angle spinning nuclear magnetic resonance (MASNMR) spectroscopy. In particular, the influence of curing conditions (time and temperature of reaction) has been analyzed. NMR results show the formation of amorphous tecto-silicates in which the amount of aluminum decreases in the two consecutive formed phases. The Si/Al ratio of zeolite precursor obtained at 85°C changes from 0.95 to 1.86 when curing time is increased from 5 h to 1 week. The evolution of the mechanical properties of prepared cements has also been discussed in terms of the phases formed and texture and morphology of samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1551-2916.2004.01141.x
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  • 3
    Electronic Resource
    Electronic Resource
    Structure of Calcium Silicate Hydrates Formed in Alkaline-Activated Slag: Influence of the Type of Alkaline Activator (2003)
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 86 (2003), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The influence of the alkaline activator (NaOH, waterglass, or Na2CO3) on the structure of the hydrated calcium silicate formed in alkali-activated slag (AAS) cement pastes has been investigated by FTIR, 29Si and 27Al magic-angle scattering nuclear magnetic resonance, and TEM/EDX techniques. In all cases, the main product formed after 7 d of activation, with activators giving an Na2O concentration of 4%, is a semicrystalline calcium silicate hydrate with a dreierkette-type anion. In these structures, linear finite chains of silicate tetrahedra (Q2 units) are linked to central Ca-O layers, and tetrahedral aluminum occupies bridging positions in the chains. The main chain length and the amount of aluminum incorporated in the tetrahedral chains depend on the activator used. The detection of Q3 silicon entities in alkaline-activated slags is discussed in relation to the possible formation of cross-linked structures that may be responsible for increased flexural and compressive strengths in AAS mortars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1151-2916.2003.tb03481.x
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in Texture and Cell Wall Polysaccharides of Olive Fruit during "Spanish Green Olive" Processing (1995)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 43 (1995), S. 2240-2246 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00056a051
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  • 5
    Electronic Resource
    Electronic Resource
    Specific diadenosine pentaphosphate receptor coupled to extracellular regulated kinases in cerebellar astrocytes (2002)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 83 (2002), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In this study, we show specific intracellular responses evoked by the stimulation of astrocytes with the P1,P5-di(adenosine-5′)pentaphosphate, Ap5A. The stimulation of astrocytes with micromolar concentrations of the dinucleotide elicited rapid increases in intracellular calcium concentration ([Ca2+]i), showing an EC50 value of 15.27 ± 0.61 µm. Moreover, the stimulation of cells with nanomolar concentrations of Ap5A, unable to induce calcium responses, increased the phosphorylated forms of extracellular-signal regulated kinase 1/2 (ERK) with an EC50 value of 9.8 ± 2.4 nm. The maximal activation was observed at 100 nm Ap5A, which was similar to that produced by epidermal growth factor (EGF) under the same experimental conditions. The present data reported here indicate that Ap5A mediated these effects by interacting with a specific receptor, not yet identified, which was different from the P2Y1 and P2Y2/P2Y4 receptors present in all individual astrocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2002.01111.x
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  • 6
    Electronic Resource
    Electronic Resource
    Acute lymphangitic nocardiasis (1973)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 89 (1973), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The third case of lymphangitic nocardiasis caused by Nocardia brasiliensis to be recorded in Uruguay is presented.The clinical picture showed some of the features of sporotrichosis, but it was more acute, the nodules developing rapidly into abscesses.A review of eight similar cases, reported previously, reveals that N. brasiliensis was properly identified in five of them. All cases were localized on the upper limbs, and granules were not seen on direct examination.Mycetomas caused by N. brasiliensis have not been observed in Uruguay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1973.tb02979.x
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  • 7
    Electronic Resource
    Electronic Resource
    Activity of cell wall-associated enzymes in ripening olive fruit (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 93 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Enzymatically active cell wall isolaled from olive (Olea europaea) fruit was employed Hi investigate some hydrolytic enzymes bound to the cell wall and the changes in these during ripening. Seven glycosidases. β-glucosidase (EC 3.2.1.21) α-galactosidase (EC 3.2.1.22). β-galactosidase (EC 3.2.1.23). α-arabinosidase (EC 3.2.1.55), α-mannosidase (EC 3.2.1,24). β-xylosidase (EC 3.2.1.37) and β-N-acetylglucosamidase (EC 3.2.1.30). as well as Cx-cellulase (EC 3.2.1.4) and endo-polygalacturonase (EC 3.2.1.15). were identified in the cell wall preparation, at four stages of ripeness (mature green. changing colour, black and black-ripe). Activities of all these cell wall-associated enzymes fionicallv and covalently linked) were determined either by cell wall incubation with artificial substrate or after extraction from the cell wall with buffers of high salt concentration (Cx-cellulase). and were compared to those of forms solubilized from acetone powders with 500 nM citrate buffer (cytoplasmic and/or apoplastic plus ionically hound to cell wall) In general, the activities of low ionic strength buffer-soluble enzymes were found to be much higher than those of the bound enzymes. The bound enzymes are present in the fruit at the green colour stage, whereas the activities of the soluble enzymes only increased from the changing colour stage onwards. The tenacity of binding of enzymes to the wall was investigated by treating the walls with high salt and measuring residual activity. The nature of the ionic and covalent binding and the changes during ripening were also established for wall-hound glycosidase During ripening there was a marked change in the percentages of covalently- and tonically linked activities of β-glucosidase and β-galaclosidase: al the changing colour stages about 75–80% of the bound active in was present in high ionic strength buffer while al the black-ripe stage it was only 15–20. A possible role for these cell wall degradative enzymes in olive softening is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb05113.x
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  • 8
    Electronic Resource
    Electronic Resource
    Cell Wall Composition of Olives (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 59 (1994), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cell walls of olives (Olea europaea L.), Hojiblanca and Manzanilla, were isolated and fractionated into polysaccharides, and compositions compared. Pectic and hemicellulosic fractions were purified by ion exchange and gel filtration chromatography, and neutral sugar and uronic acid composition determined. Differences occurred between cultivars and seasons: Manzanilla had higher pectic polysaccharides and lower xylans. Hojiblanca showed similar but lesser differences. Arabinans were the main neutral pectic polysaccharides with arabinose 〉 80%. Homogalacturonans and rhamnogalacturonans were 〉 50% of the acidic pectic fractions. Degree of esterification and molecular weights were related to extracting solvent. Xyloglucans and galactoglucomannans were neutral hemicelluloses with molecular weights ≅ 260 kD. Glucuronoarabinoxylans had higher molecular weights (up to 400 kD). Acidic xylans were important in the pulp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1994.tb14674.x
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  • 9
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    Unknown
    José Miguel LORENZO ARRIBAS, "Hildegarda de Bingen (1098-179)" (Book Review) (1995)
    Barcelona : Periodicals Archive Online (PAO)
    Anuario de estudios medievales. 25:2 (1995) 840 
    Details
    ISSN: 0066-5061
    Topics: History
    Description / Table of Contents: RESEÑAS
    Notes: BIBLIOGRAFÍA
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:h372-1995-025-02-000024
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  • 10
    Electronic Resource
    Electronic Resource
    Activity of glycosidases during development and ripening of olive fruit (1993)
    Springer
    European food research and technology 196 (1993), S. 147-151 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Summary We have studied the activity of variousp-nitrophenyl-d-glycosidases during development and ripening of olive fruit in three seasons (1987–88, 1988–89, 1989–90). Activity was absent in the first phases of ripening but appeared in the completely developed fruit,α-Galactosidase,β-galactosidase,α-mannosidase,α-arabinosidase, andα-xylosidase activities were detected and their relative molecular mass determined by gel filtration chromatography using Sephadex G-100. There is a temporal correlation between enzymatic activity and decrease in non-cellulosic cell-wall neutral sugar composition.
    Notes: Zusammenfassung Es wurde die Aktivität von verschiedenenp-Nitrophenyl-d-glycosidasen während der Entwicklung und Reifung der Oliven in drei Jahren (1987–88; 1988–89, 1989–90) untersucht. Es wurde keine Aktivität in der voll entwickelten Frucht festgestellt. Es wurden die Aktivitätenα-Galactosidase,β-Galactosidase,α-Mannosidase,α-Arabinosidase undα-Xylosidase entdeckt und ihre Molekularmassen durch Gelfiltration-Chromatographie mit Sephadex G-100 bestimmt. Es gibt eine zeitliche Wechselwirkung zwischen der enzymatischen Aktivität und der Abnahme in der Zuckerzusammensetzung in dem Nichtcellulose-Anteil der Zellwand.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01185575
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