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Effect of spatial arrangement of the basement membrane on cultured pleomorphic adenoma cells. Study by immunocytochemistry and electron and confocal microscopy (1997)

Jaeger, Márcia M. M. ; Araújo, Vera C. ; Kachar, Bechara ; [et al.]

Springer

Virchows Archiv 430 (1997), S. 467-477

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ISSN: 1432-2307

Keywords: Key words Salivary gland neoplasms ; Myoepithelial cell ; Extracellular matrix ; Matrigel

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract In a cell line from human pleomorphic adenoma (AP2 cells) we studied the response of these cells to basement membrane proteins. The culture was characterized as myoepithelial-like by transmission electron microscopy and immunocytochemistry. AP2 cells were grown in contact with a reconstituted basement membrane (Matrigel). Cells grown on Matrigel showed conspicuous phenotypic alterations, depending on how the substrate was applied. Cells grown on the top of Matrigel developed a dendritic phenotype, exhibiting thin, long and intercommunicating cytoplasmic extensions resembling normal myoepithelial cells. Cells grown inside Matrigel formed multi-layered clusters. Light, confocal and transmission electron microscopy showed that these clusters were formed by double-layered epithelioid cells delimiting luminal spaces. The cells facing the lumen were cuboidal, showing microvilli at the apical plasmalemmal and junctional complexes. The spatial arrangement of basement membrane is a key modulator of morphogenetic changes and cytodifferentiation of tumour myoepithelial cell lineage in culture.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004280050057

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Analysis of the interdependent localization of vimentin and microtubules in neoplastic myoepithelial cells (1995)

Jaeger, Ruy G. ; Jaeger, Márcia M. M. ; Araújo, Vera C. ; [et al.]

New York, NY : Wiley-Blackwell

Cell Motility and the Cytoskeleton 32 (1995), S. 289-298

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ISSN: 0886-1544

Keywords: cytoskeleton ; intermediate filaments ; vimentin ; microtubules ; myoepithelial cells ; immunofluorescence ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Salivary gland neoplastic myoepithelial cells in culture form very thin cytoplasmic processes in which the vimentin network is well dispersed. These vimentin filaments can be individually visualized by immunofluorescence. In this study, we have analyzed the role of microtubules in the distension and organization of the vimentin filament network found in these cells. We find that vimentin filaments colocalize along microtubules; however, a significant number of filaments can also be found in microtubule-free domains. Additionally, vimentin filaments are absent from large domains of microtubule inhibitor nocodazole did not cause any retraction of the distended vimentin network. This observation suggests that the structural integrity of microtubules is not important for the stability of the vimentin network. Combining procedures for transient disruption of vimentin filaments and microtubules we observed that, in the absence of microtubules, the vimentin network could reassemble in the perinuclear region but was unable to extend toward the cell periphery. The dispersion of vimentin filaments to the peripheral regions of the cytoplasm could only be observed upon microtubule reassembly. This indicates that microtubules are not required for the stability of the vimentin network, but the dispersion of vimentin filaments to the peripheral cytoplasm depends on active interactions with microtubules. © 1995 Wiley-Liss, Inc.

Additional Material: 7 Ill.