ISSN:
1432-041X
Keywords:
Key words Drosophila
;
pair-rule gene
;
phenocopy
;
even skipped
;
CALI
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The even skipped (eve) gene in Drosophila encodes a homeo-domain protein that acts as a trancriptional regulator during early embryogenesis. We show that an injection of a monoclonal antibody against the eve homeodomain in conjunction with chromophore-assisted laser inactivation (CALI) precisely phenocopies the eve mutant phenotype. Depending on the time of the laser treatment, both the early pair-rule function, as well as the later segmental function of eve can be blocked. This suggests that it might be possible to employ CALI to analyse the function of transcriptional regulators in species that are not amenable to genetic analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004270050034
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