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  • 1
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of different concentrations of magnesium, potassium, phosphorus and copper on the growth kinetics of the methanotrophic bacterium GB 25 was investigated. Diverse inhibition models were used for the mathematical description of the results. On the basis of these model equations the optimal concentrations of the investigated ions were derived. Copper causes the greatest inhibition, on the contrary, potassium practically does not inhibit the growth of bacterium GB 25.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 13-19 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A thermotolerant yeast species of Lodderomyces elongisporus EH 60 was isolated and physiologically characterized. This yeast possesses a high specific growth rate with μmax = 0.61 h-1. The dependence of the specific growth rate and cell yield on temperature, dilution rate, sucrose concentration and pH-value is investigated. Sucrose concentrations greater than 10 g/l inhibit the growth velocity.The specific growth rate μ can be calculated by a simple combination equation in the form: \documentclass{article}\pagestyle{empty}\begin{document}$$ \begin{array}{*{20}c} {\mu \, = \,\mu _{\max } \frac{{\rm S}}{{{\rm K}_{\rm s} \, + \,{\rm S}}}\, - \,K_2 \cdot S^{K_1 } } \\ {{\rm (growth term)}\,{\rm (inhibition term)}} \\ \end{array} $$\end{document}.The total optimum values for a sucrose-based continuous growth process with regard to the optimum cell yeild are: YS = 0.50 g DM/g S. Topt. = 38,6 °C and Dopt. = 0,35 h-1. The function YS = f(D, T) is represented by a total model.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 11 (1991), S. 303-314 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In a process running more than five months it was proved that a nonsterile processing is possible at a stable microbial composition as well as stable performance parameters like productivity, growth yield, and product quality.For the stable processing it is important that methanol and dissolved oxygen concentration must not simultaneously exceed the threshold values (methanol〉 1 g/1; dissolved oxygen 〉 0,5 mg/1).The postulated transfer rate obstructs at higher biomass concentrations did not occur in the examined ranges up to 80 kg biomass/1. Consequently, the growth rate did not depend on high biomass concentrations in this range.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 13 (1993), S. 111-115 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The growth rate and the maximum cell concentration of methanotrophic bacteria are limited by the transfer of methane and oxygen to the culture fluid. The operation under moderate pressure results in an increase in driving force for the mass transfer of both nutrients and, therefore, in a large increase in the attainable biomass concentration.Our laboratory pressure fermenter with a volume of 12 litres operates under a system pressure of up to 0.5 MPa. In this reactor a maximum productivity of 6 g biomass/lh is achieved. However, operating under moderate system pressure and exhaust gas recycling has also disadvantages because the concentrations of the gas phase components may inhibit the growth process.From the results of the laboratory fermenter we have developed kinetic models of the influence of dissolved oxygen and carbon dioxide on the specific growth rate of the methanotrophic strain GB 25. These models are the basis for processing under increased system pressure and exhaust gas recycling.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 499-503 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A quadrupol mass spectrometer QMS c 300 developed and manufactured from the workshops of the Institute of Nuclear Research of the Hungarian Academy of Sciences, Debrecen, has been cuppled with a 12 l laboratory steril fermenter. The QMS apparatus is provided with 3 different possibilities for sampling. Via a dynamic air input system the discharged air of the fermenter can be analyzed continuously. The static sampling input allows the off-line measurement of gaseous and volatile compounds. Special membran samplers are used for measurements of volatile and gaseous substrates and microbial metabolites in the fermentation broth. The samplers can be adopted in the reactor vessel directly analogous to the pH and pO2-electrodes.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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