ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Measurements of the frequency of human erythrocytes with gene expression loss phenotypes at the glycophorin A locus (1986)

Langlois, R. G. ; Bigbee, W. L. ; Jensen, R. H.

Springer

Human genetics 〈Berlin〉 74 (1986), S. 353-362

add to mindlist on the mindlist

Details

ISSN: 1432-1203

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary An assay method is described for determining the frequency of human erythrocytes having a gene expression loss phenotype at the glycophorin A locus presumably due to in vivo somatic mutational events in erythroid precursor cells. Monoclonal antibodies specific for the M and N glycophorin A alleles are used to identify variant cells that lack the expression of one allele in blood samples from MN heterozygotes. Flow cytometry and sorting are used to enumerate and purify variant cells. Using three different antibody combinations which are sensitive to the loss of either the M or the N allele, we find that variant cells occur at a frequency of 1x10-5 in normal donors. We also detect variant cells with an apparent homozygous phenotype suggesting that events leading to homozygosity may occur at similar frequencies to gene loss events. Significant increases in variant cell frequency are observed in cancer patients after exposure to mutagenic chemotherapy drugs.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00280485

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Pressure drop and holdup in stratified gas-liquid flow (1974)

Russell, T. W. F. ; Etchells, A. W. ; Jensen, R. H. ; [et al.]

Hoboken, NJ : Wiley-Blackwell

AIChE Journal 20 (1974), S. 664-669

add to mindlist on the mindlist

Details

ISSN: 0001-1541

Keywords: Chemistry ; Chemical Engineering

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A mathematical model and an iterative procedure to calculate holdup and pressure drop in horizontal gas-liquid flow is developed. The predictions of the model agree with well over a hundred data points collected with air-water and air-glycerine solutions in 0.0254-, 0.0381-, and 0.0508-m. diameter pilot pipelines. A design procedure using the verified model is presented.

Additional Material: 7 Ill.