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  • 1
    Electronic Resource
    Electronic Resource
    The contribution of HLA-Class II antigens in humoral non-response and delayed response to HBsAg vaccination (2000)
    Springer
    Pflügers Archiv 440 (2000), S. r188 
    Details
    ISSN: 1432-2013
    Keywords: Key words HBsAg ; vaccination ; nonresponsiveness ; HLA ; PCR-based HLA typing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The variability in the immune response modulated by HLA alleles may be an important factor for the induction of the protective effect of HBsAg vaccines. We present here the analysis of HLA-DRB1, DQB1 and DQA1 alleles and their combinations in the group of 36 individuals with poor humoral immmune response to HBsAg vaccination. Comparison with the control group, consisted of 60 randomly choosen healthy subjects, revealed that the DRB1*1601, DQB1*0502, DQA1*0102 haplotype is overrepresented in the group of hyporesponders and may therefore be regarded as a factor influencing poor antibody responsiveness. We observed that after revaccination two of three individuals who failed to develop anti-HBs antibodies carry the same phenotype DRB1*0101,DRB1*0301;DQB1*0501, DQB1*0201;DQA1*0101,DQA1*0501, which supports the conjecture that immunogenicity of the HBsAg vaccine depends on specific combination of HLA DR and DQ molecules on antigen presenting cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240000059
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  • 2
    Electronic Resource
    Electronic Resource
    Vitamin C induced apoptosis in human articular chondrocytes (2000)
    Springer
    Pflügers Archiv 440 (2000), S. r046 
    Details
    ISSN: 1432-2013
    Keywords: Key words vitamin C (L-ascorbic acid) ; apoptosis ; human articular chondrocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Chondrocytes present in articular cartilage survive as a resident cell population throughout the lifespan of the individual organism. However, articular chondrocytes as other cells also undergo apoptosis and there is an ever increasing list of diverse stimuli that can induce this phenomenon in vitro. Our main interest was to investigate potential cytotoxic effects of vitamin C (L-ascorbic acid) on human articular chondrocytes. The present study suggests that vitamin C can induce apoptosis in a cell culture of chondrocytes after 18 h of cultivation. Apoptosis-inducing activity of L-ascorbic acid is dose dependent and significantly affected by the presence of serum. The increased number of vitamin C induced apoptotic cells was associated with DNA fragmentation and morphological changes of the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240000001
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    In vitro functional tests for evaluation of stimulating capacity of cultured human dendritic cells (2000)
    Springer
    Pflügers Archiv 440 (2000), S. r049 
    Details
    ISSN: 1432-2013
    Keywords: Key words dendritic cells-calcium ionophore ; maturation ; mixed lymphocyte reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Basic functional test for evaluation of in vitro cultured human dendritic cells (DC) is primary allogeneic one-way mixed lymphocyte reaction (MLR). In this way, one can evaluate stimulating capacity, which is a basic characteristic of DC. The proliferation of cells is measured through incorporation of 3H-thymidine. Normally proliferation is measured at days 5-7. We studied kinetics of proliferative responses initiated with different stimulating cell suspensions to evaluate differences and possibly reduce time needed to perform this test. Gradual increase in response from days 1 to 7 and a significant difference from controls (peripheral blood mononuclear cells) seen from day 4 was noted if macrophages were used as stimulators. A consistently higher proliferation, compared to controls, was always found already on day 2 when mature DC were used as stimulators. The reaction peaked 2 to 3 days earlier and was also more than two times more intense. This maximal and significantly higher response, consistently seen already after 48 hours, allows us to confirm the presence of mature DC in stimulating suspensions much earlier than previously.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240000002
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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