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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical analysis of steroid receptors and glycodelin A (PP14) in isolated glandular epithelial cells of normal human endometrium (2000)
    Springer
    Histochemistry and cell biology 114 (2000), S. 405-411 
    Details
    ISSN: 1432-119X
    Keywords: Endometrium Glandular cells Estrogen receptor (ER) Progesterone receptor (PR) Glycodelin A (PP14)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Highly purified fractions of isolated endometrial cells can be useful for investigating endometrial function. After a first collagenase digestion, normal human endometrial stromal and epithelial cells were separated by filtration. Glands were purified further by two collagenase digestion steps, filtration, differential sedimentations, and Ficoll gradient centrifugation. Epithelial cells were polyhedral and grew as islands in a whorl-like wavy pattern around glandular fragments. High cell culture purity was confirmed with the positive immunohistochemical reaction against cytokeratin 7,8,18,19. Isolated human glands had a similar distribution pattern of estrogen receptor (ER) and progesterone receptor (PR) as observed in vivo, suggesting that glands have a functional hormone receptor system at the time of plating. Using a specific monoclonal antibody against glycodelin A (GdA), a characteristic cyclical expression was demonstrated during the menstrual cycle. The GdA reaction was weak in the proliferative phase, increasing significantly till the late secretory phase, suggesting a similar GdA concentration in vitro as observed in vivo glands. In conclusion, this method could be a model for studying endometrial glandular cells from different menstrual phases, endometrial cell interactions, implantation mechanisms, GdA regulation mechanisms, and pharmacological or other influences on ER and PR alteration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180000196
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibin/activin subunits alpha, beta-A and beta-B are differentially expressed in normal human endometrium throughout the menstrual cycle (2000)
    Springer
    Histochemistry and cell biology 122 (2000), S. 461-471 
    Details
    ISSN: 1432-119X
    Keywords: Endometrium ; Normal ; Immunohistochemistry ; Immunofluorescence ; Inhibin/activin subunits ; Inhibin-alpha ; Inhibin-beta A ; Inhibin-beta B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: AbstractInhibins are dimeric glycoproteins composed of an alpha (α) subunit and one of two possible beta (β-) subunits (βA or βB). The aims of this study were to assess the frequency and tissue distribution patterns of the inhibin subunits in normal human endometrium. Samples from human endometrium from proliferative phase (PP; n=32), early secretory phase (ES; n=10) and late secretory phase (LS; n=12) were obtained. Immunohistochemistry, immunofluorescence and a statistical analysis were performed. All three inhibin subunits were expressed by normal endometrium by immunohistochemistry and immunofluorescence. Inhibin-α was primarily detected in glandular epithelial cells, while inhibin-β subunits were additionally localised in stromal tissue. Inhibin-α staining reaction increased significantly between PP and ES (P〈0.05), PP and LS (P〈0.01), and ES and LS (P〈0.02). Inhibin-βA and -βB were significant higher in LS than PP (P〈0.05) and LS than ES (P〈0.05). All three inhibin subunits were expressed by human endometrium varying across the menstrual cycle. This suggests substantial functions in human implantation of inhibin-α subunit, while stromal expression of the β subunits could be important in the paracrine signalling for adequate endometrial maturation. The distinct expression in human endometrial tissue suggests a synthesis of inhibins into the lumen and a predominant secretion of activins into the stroma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00418-004-0709-6
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  • 3
    Electronic Resource
    Electronic Resource
    D-Galacturonsäurederivate, VI. D-Galacturonsäurederivate mit flüssigkristallinen Eigenschaften (1992)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1992 (1992), S. 1171-1177 
    Details
    ISSN: 0170-2041
    Keywords: Carbohydrates ; Amphiphilic d-galacturonic acid derivatives ; Liquid crystals ; Discotics ; Calculations, MM2 ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: D-Galacturonic Acid Derivatives, VI.  -  D-Galacturonic Acid Derivatives with Liquid Crystalline PropertiesEsters, thioesters, and amides of D-galacturonic acid have been synthesized from alcohols, thioalcohols, and amines having long alkyl chains. Key intermediate of the reaction with the different nucleophiles was the 1,2;3,4-di-O-isopropylidene-α-D-galactopyranuronosyl chloride (3). Deisopropylidenation of the protected esters, thioesters, and amides was achieved by trifluoroacetic acid. The investigation of the liquid crystalline properties was carried out with a polarizing microscop, DSC measurements, and X-ray diffraction. Molecules with three long alkyl chains can be obtained by reaction of amides 5h and 5i with thioalcohols. MM2 calculations voted a Y-shaped structure of the mercaptals 8a and 8b which do exhibit thermotropically a columnar hexagonal mesophase (Dhd) as we have proved by X-ray diffraction.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.1992199201192
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  • 4
    Electronic Resource
    Electronic Resource
    Galacturonic Acid Derivatives, VIIIPart VII: See ref.[1].. Synthesis of N-(D-Galacturonoyl) Amino Acids and DipeptidesThis paper is dedicated to Professor Dr. Hans Paulsen on the occasion of his 75th birthday. (1997)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1997 (1997), S. 737-743 
    Details
    ISSN: 0947-3440
    Keywords: D-Galacturonic acid ; Amino Acids ; Amides ; Dipeptides ; Carbohydrates ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: D-Galacturonic acid derivatives, amide-linked to amino acids or dipeptides, have been synthesized. These glycoconjugates exhibit stability when the uronic acid residue is protected with isopropylidene and acetyl groups or is in its pyranoside form. The formation of the amide or peptide linkages was best achieved by the EDCI/HOBt coupling method; an alternative method employing the 1,2;3,4-di-O-isopropylidene-α-D-galactopyranuronosyl chloride 2 furnished only moderate yields of the conjugates. The use of NMR spectroscopy to detect racemization of the amino acid moiety is also discussed herein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199719970416
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