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  • 1
    Electronic Resource
    Electronic Resource
    Effect of human fibroblast-derived dermis on expansion of tissue from venous leg ulcers (2003)
    Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 11 (2003), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Novel approaches to healing of chronic wounds, such as venous leg ulcers, include the use of tissue-engineered skin substitutes, e.g., human fibroblast-derived dermis. The exact mechanisms of action of these products and their effects on wound healing at a cellular level are yet to be fully defined. The aim of our study was to evaluate the potential effects of human fibroblast-derived dermis on the healing of chronic wounds using an experimental model. We used a tissue expansion model to examine the effect of human fibroblast-derived dermis on the growth of human tissue biopsied from venous leg ulcers. Further characterization of the cytokine profile produced by human fibroblast-derived dermis in culture was performed using enzyme-linked immunosorbent assay techniques. Addition of medium conditioned with human fibroblast-derived dermis significantly increased the outgrowth of cells from venous leg ulcer biopsies (p = 0.001). We detected bioactive levels of hepatocyte growth factor/scatter factor and interleukin-8 in media conditioned with human fibroblast-derived dermis. Therefore, conditioned media from human fibroblast-derived dermis enhances ex vivo expansion of tissue taken from chronic venous leg ulcers, and contains potent angiogenic factors. These experimental findings may explain the enhanced healing seen with clinical applications of human fibroblast-derived dermis on chronic wounds. (WOUND REP REG 2003;11:292–296)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1524-475X.2003.11409.x
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  • 2
    Electronic Resource
    Electronic Resource
    Phenotypic variation in the production of bioactive hepatocyte growth factor/scatter factor by oral mucosal and skin fibroblasts (2001)
    Oxford, UK : Blackwell Science Inc
    Wound repair and regeneration 9 (2001), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic growth factor produced principally by cells of mesenchymal origin. HGF/SF is an important mitogen, morphogen, and motogen and plays an important role in wound healing, tumorigenesis and particularly fetal development. Oral mucosal fibroblasts exhibit a fetal phenotype, including an increased extracellular matrix reorganizational ability, cellular migration and experimental wound repopulation in comparison to skin fibroblasts. In this study the expression, production, and bioactivity of HGF/SF by oral mucosal and skin fibroblasts was investigated. Although both oral mucosal and skin fibroblasts expressed HGF/SF, the oral mucosal fibroblasts produced significantly increased amounts of total HGF/SF (p 〈 0.01) as measured by enzyme-linked immunosorbent assay and bioactive HGF/SF as measured by cell scatter and cell-dissociation techniques (p 〈 0.01). The possible effect of increased HGF/SF in production mediating the previously described preferential responses of oral mucosal fibroblasts was studied in vitro. Reverse transcriptase-polymerase chain reaction–Western blotting and immunocytochemistry methods all showed that both oral mucosal and skin fibroblasts expressed and produced the c-Met receptor. Recombinant HGF (20–40 ng/mL) however, failed to affect fibroblast repopulation of monolayer wounds or cellular proliferation. In contrast, recombinant HGF significantly increased ECV304 wound repopulation. These studies provide direct evidence of another mechanism by which site-specific variations in fibroblast phenotype may contribute in a paracrine fashion to the rapid reepithelialization and revascularization of oral wounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1524-475x.2001.00034.x
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  • 3
    Electronic Resource
    Electronic Resource
    Electrical and photoluminescent properties of high-quality GaSb and AlGaSb layers grown from Sb-rich solutions by liquid-phase epitaxy (1995)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 77 (1995), S. 1725-1728 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The growth-temperature dependence of electrical and photoluminescent (PL) properties of GaSb and Al0.065Ga0.935Sb layers grown from Sb-rich solutions by liquid-phase epitaxy has been studied. The GaSb and AlGaSb epitaxial layers grown at 606 °C exhibit a low hole concentration of 4×1015 and 2×1016 cm−3, respectively. The low-temperature PL spectra of GaSb and AlGaSb is dominated by free-exciton transition and excitons bound to neutral acceptors. As the growth temperature is increased, both the residual hole concentration and the intensity of band A related to native lattice defects in GaSb and AlGaSb increase. The high-quality GaSb and AlGaSb epitaxial layers can be grown at low temperatures from Sb-rich solutions. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.359576
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  • 4
    Electronic Resource
    Electronic Resource
    Optical properties of GaSb alloys and photodiodes grown by liquid-phase epitaxy (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 80 (1996), S. 1731-1734 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The carrier concentration of GaSb epitaxial layers grown from Sb-rich solutions by liquid-phase epitaxy is controlled by replacing nominally undoped GaSb by Te-doped polycrystalline GaSb to suppress or compensate the background hole concentration. The dependence of the 10 K photoluminescence peak wavelength and intensity on the doping level of the Te-compensated GaSb layers has been investigated. The absorption spectra are examined as a function of carrier concentration. Low-dopant-concentration p-type GaSb samples exhibit the band gap shrinkage with increasing hole concentration, while the lightly Te-doped n-type GaSb samples exhibit a band gap increase due to the Burstein–Moss shift with increasing electron concentration. By using Te-doped GaSb layers with an electron concentration of 5.6×1015 cm−3, the fabricated GaSb photodiodes exhibit a low dark current of 2 μA at −5 V, a high breakdown voltage of 28.7 V at 20 μA, and a maximum photoresponsivity of 0.55 A/W with an external quantum efficiency of 40% at 1.72 μm wavelength. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.362972
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  • 5
    Electronic Resource
    Electronic Resource
    High-frequency resonance in acoustic superlattice of LiNbO3 crystals (1988)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 53 (1988), S. 2278-2280 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The relationship between the resonance frequency and the periodicity of the acoustic superlattice of LiNbO3 crystals has been obtained by analysis of the excitation and propagation of elastic waves in this kind of material with which high-frequency resonance in the range of 500–800 MHz has been realized experimentally. The experimental results are in good agreement with the theoretical ones. The applications of acoustic devices operating at frequencies high above 500 MHz are to be expected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.100514
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  • 6
    Electronic Resource
    Electronic Resource
    Acoustic superlattice of LiNbO3 crystals and its applications to bulk-wave transducers for ultrasonic generation and detection up to 800 MHz (1988)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 53 (1988), S. 1381-1383 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In this letter theoretical calculations and experimental results of ultrasonic generation and detection up to 800 MHz using an acoustic superlattice of LiNbO3 crystals are reported. The experiment is in good agreement with theory. Transducers with an insertion loss of nearly 0 dB at 555 MHz and a 5.8% 3 dB bandwidth have been made.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.100455
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  • 7
    Electronic Resource
    Electronic Resource
    Endosperm-specific expression of green fluorescent protein driven by the hordein promoter is stably inherited in transgenic barley (Hordeum vulgare) plants (2002)
    Oxford, UK : Blackwell Science, Ltd
    Physiologia plantarum 115 (2002), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic barley (Hordeum vulgare L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by either a rice actin promoter or a barley endosperm-specific d-hordein promoter. The gene encoding phosphinothricin acetyltransferase (bar), driven by the maize ubiquitin promoter and intron, was used as a selectable marker to identify transgenic tissues. Strong GFP expression driven by the rice actin promoter was observed in callus cells and in a variety of tissues of T0 plants transformed with the sgfp(S65T)-containing construct. GFP expression, driven by the rice actin promoter, was observed in 14 out of 17 independent regenerable transgenic callus lines; however, expression was gradually lost in T0 and later generation progeny of diploid lines. Stable GFP expression was observed in T2 progeny from only 6 out of the 14 (43%) independent GFP-expressing callus lines. Four of the 8 lines not expressing GFP in T2 progeny, lost GFP expression during T0 plant regeneration from calli; one lost GFP expression in the transition from the T0 to T1 generations and three lines were sterile. Similarly, expression of bar driven by the maize ubiquitin promoter was lost in T1 progeny; only 21 out of 26 (81%) independent lines were Basta-resistant. In contrast to actin-driven expression, GFP expression driven by the d-hordein promoter exhibited endosperm-specificity. All seven lines transformed with d-hordein-driven GFP (100%) expressed GFP in the T1 and T2 generations, regardless of ploidy levels, and expression segregated in a Mendelian fashion. We conclude that the sgfp(S65T) gene was successfully transformed into barley and that GFP expression driven by the d-hordein promoter was more stable in its inheritance pattern in T1 and T2 progeny than that driven by the rice actin promoter or the bar gene driven by the maize ubiquitin promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2002.1150117.x
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  • 8
    Electronic Resource
    Electronic Resource
    Mast cell density and IL-8 expression in nonlesional and lesional psoriatic skin (2001)
    Oxford, UK : Blackwell Science Ltd
    International journal of dermatology 40 (2001), S. 0 
    Details
    ISSN: 1365-4632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: AbstractBackground  An important cellular aberration at sites of psoriatic inflammation is an increase in the number of dermal mast cells. Being multifactorial immune effector cells, it is believed that mast cells play an essential role in perpetuating the inflammatory process of psoriasis. However, factors responsible for the infiltration and accumulation of mast cells in psoriatic lesions are largely unknown. Recent studies have demonstrated that Interleukin-8 (IL-8) exerts strong chemotactic effects on mast cells in vitro. Overexpression of IL-8 has also been reported in psoriatic lesions. In this study, we have found a correlation between the expression of IL-8 and dermal mast cell density in lesional psoriatic skin as compared to nonlesional psoriatic skin.Methods  Four-mm punch biopsies were taken from 14 psoriatic patients and eight healthy volunteers. Using immunohistochemical techniques, 8 μm sections of lesional psoriatic, nonlesional psoriatic, and normal control samples were evaluated for dermal mast cell density and the density of IL-8 expressing keratinocytes.Results  It was found that dermal mast cell density in lesional psoriatic, nonlesional psoriatic, and normal skin was 105.4 ± 71.2, 42.3 ± 30.1, and 47.5 ± 32.5 mast cells/mm2, respectively. IL-8+ keratinocyte density in lesional psoriatic, non lesional psoriatic, and normal skin was 171.5 ± 67.1, 25.4 ± 14.9 and 20.6 ± 8.7 IL-8+ Keratinocytes/mm2, respectively.Conclusions  The results of this study suggest that increased levels of IL-8 in the keratinocytes of psoriatic plaques play a contributing role in the migration of mast cells to lesion sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-4362.2001.01262.x
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  • 9
    Electronic Resource
    Electronic Resource
    Disruption of PTEN coupling with 5-HT2C receptors suppresses behavioral responses induced by drugs of abuse (2006)
    [s.l.] : Nature Publishing Group
    Nature medicine 12 (2006), S. 324-329 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The widespread distribution of the tumor suppressor PTEN (phosphatase and tensin homolog deleted on chromosome 10) in the adult brain suggests its role in a broad range of brain functions. Here we show evidence supporting a physical interaction of PTEN with a region in the third intracellular loop ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm1349
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  • 10
    Electronic Resource
    Electronic Resource
    KILLER/DR5 is a DNA damage–inducible p53–regulated death receptor gene (1997)
    [s.l.] : Nature Publishing Group
    Nature genetics 17 (1997), S. 141-143 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] P53 (also known as TP53), the cellular ‘gatekeeper for growth and division’1, is the most commonly mutated gene in human cancer, and its inactivation contributes not only to tumour progression but also to resistance of cancer cells to chemotherapy2,3. p53 normally controls cell ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1097-141
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