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  • 1
    Electronic Resource
    Electronic Resource
    Transcriptional regulation of hydroxypyruvate reductase gene expression by cytokinin in etiolated pumpkin cotyledons (1996)
    Springer
    Planta 198 (1996), S. 1-5 
    Details
    ISSN: 1432-2048
    Keywords: Cucurbita ; Cytokinin ; Gene expression ; Hydroxypyruvate reductase ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To understand the mechanisms by which the expression of a specific gene is modulated by cytokinin, the regulation of hydroxypyruvate reductase (HPR) transcript levels by N6-benzyladenine (BA) in etiolated pumpkin (Cucurbita pepo L. cv. Halloween) cotyledons was investigated. A pumpkin HPR cDNA was generated by reverse transcriptase-polymerase chain reaction and its nucleotide sequence was determined. An antisense HPR RNA was prepared for RNase protection analysis of HPR-mRNA expression patterns in the cotyledons of dark-grown pumpkin seedlings. Treatment of the cotyledons with BA was shown to modulate HPR mRNA levels in a dose- and time-dependent manner. Similarly, nuclear run-on studies showed that the rate of transcription was also enhanced by BA treatment of the cotyledons. These results suggest that the enhancement of HPR mRNA by cytokinin is, at least in part, at the level of transcription.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197579
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of DNA-binding proteins with the 5′-flanking region of a cytokinin-responsive cucumber hydroxypyruvate reductase gene (1998)
    Springer
    Plant molecular biology 38 (1998), S. 713-723 
    Details
    ISSN: 1573-5028
    Keywords: cucumber ; cytokinin-responsive ; DNA-binding proteins ; hydroxypyruvate reductase ; transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription of the cucumber hpr-A gene is responsive to cytokinin and light. To investigate the molecular basis for transcriptional regulation by cytokinin, we have identified DNA sequences and proteins that may be involved in the regulation of hpr-A gene expression. Transient expression assays in etiolated cucumber cotyledons indicate that the 315 bp fragment (−382 to −67) contains sequences necessary for cytokinin responsiveness of the luciferase reporter gene. Band shift assays detected cytokinin-enhanced and -reduced protein binding sites in a 97 bp fragment (−382 to −285) upstream of the hpr-A gene. DNase I footprinting identified two protein-protected sites, a 15 bp sequence, 5′-AAATGACGAAAATGC-3′, that contains an as-1 TGACG motif found in other plant promoters, and a 13 bp sequence, 5′-AAGATTGATTGAG-3′, of unknown function. Two-dimensional band shift analysis of the cytokinin-responsive DNA protein complex revealed the presence of six DNA protein interactions. Band shift assays showed that cytokinin and light have different effects on the interaction of nuclear proteins to the 97 bp fragment of the hpr-A gene. These data suggest that cytokinin and light do not share identical signal transduction pathways in regulating hpr-A gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006034932322
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Modeling, scheduling and control of flexible manufacturing systems by extended high-level evaluation Petri nets (1997)
    Springer
    IIE transactions 29 (1997), S. 147-158 
    Details
    ISSN: 1573-9724
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper proposes an extended high level evaluation Petri nets (EHLEP-N) for modeling and control of flexible manufacturing systems (FMSs). This new class of models is simple, perceivable and descriptive, having the capability of inference and making decisions. When applied to FMSs, the EHLEP-N can model real-time decisions with which dynamic scheduling rules can be investigated and evaluated. Experimental results show that the throughput for such systems with the new scheduling strategies can increase on an average of 7% by decreasing no-inputs and blockings compared with that of the traditional strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018506809408
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Breakthrough curves for fixed-bed adsorbers: Quasi-lognormal distribution approximation (1997)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 43 (1997), S. 979-985 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The quasi-lognormal distribution (Q-LND) approximation was used to predict breakthrough curves in fixed-bed adsorbers for a linear adsorption system with axial dispersion, external film diffusion resistance, and intraparticle diffusion resistance for slab-, cylindrical-, and spherical-particle geometries. The exact solution and parabolic profile approximation were also obtained for different particle geometries. Numerical results show that the Q-LND approximation is a simple and handy solution. It predicts breakthrough curves with an accuracy comparable to the parabolic-profile approximation over a wide range of parameters; compared with the latter, it only takes less than one hundredth the computation time and does not have a convergence problem in numerical calculations. A criterion for the applicability of the Q-LND approximation is suggested. The effect of particle geometries on the breakthrough curves is discussed. A criterion is also provided for the Q-LND approximation to explore the conditions where one should consider this effect on breakthrough curves.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690430413
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    Paper (German National Licenses)
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