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  • 1
    Electronic Resource
    Electronic Resource
    Water Content of Hydrocarbons Modified Karl Fischer Method (1950)
    s.l. : American Chemical Society
    Analytical chemistry 22 (1950), S. 555-558 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60040a012
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  • 2
    Electronic Resource
    Electronic Resource
    A case of juvenile lipidosis: Electron microscopic, histochemical and biochemical studies (1968)
    Springer
    Acta neuropathologica 11 (1968), S. 122-139 
    Details
    ISSN: 1432-0533
    Keywords: Juvenile Lipidosis ; Electron Microscopy ; Histochemistry ; Biochemistry ; Granular Lipid Inclusions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopische, histochemische und biochemische Untersuchungen eines Falles von juveniler Lipidose werden mitgeteilt. Die neuronalen Einschlüsse sind zumeist von einer unit membrane umgeben und zeigen fingerabdruckähnliche Muster. Membranovesiculäre Körperchen finden sich ebenfalls in den Nervenzellperikaryen; typische granuläre Lipofuscinkörperchen werden aber nicht gesehen. Die Astrocyten der Rinde enthalten eine ähnliche Art von Einschlüssen, von denen aber die Einschlüsse in den Astrocyten der Marksubstanz völlig verschieden sind. Die Lokalisation der Aktivität von saurer Phosphatase in allen Einschlußtypen läßt an ihre mögliche lysosomale Natur denken. Die biochemische Analyse deckt keine spezifischen Abweichungen auf.
    Notes: Summary Electron microscopic, histochemical and biochemical studies of a case of juvenile lipidosis have been presented. The neuronal inclusions were mostly unit membranebound with fingerprint-like patterns. Membrano-vesicular bodies were also found in the neuronal perikarya, but “typical” granular lipofuscin bodies were not seen. Cortical astrocytes contained a similar type of inclusion but the inclusions in the astrocytes in the white matter were quite different. The localization of acid phosphatase activity in all types of inclusions suggests their possible lysosomal nature. Biochemical analysis failed to pin-point any specific abnormalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690216
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  • 3
    Electronic Resource
    Electronic Resource
    THE P2 PROTEIN OF BOVINE ROOT MYELIN: ISOLATION AND SOME CHEMICAL AND IMMUNOLOGICAL PROPERTIES (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 23 (1974), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— A small basic protein (mol.wt. 12,000), referred to as the P2 protein, was extracted with dilute acid from delipidated bovine root myelin and purified by ion exchange chromatography on cellulose phosphate. It appeared homogeneous on polyacrylamide gel electrophoresis. The P2 protein had a distinctly different amino acid composition than the larger basic protein (mol.wt. 18,000), referred to as the P1 protein, that is also present in peripheral nerve myelin. It contained relatively more hydrophobic residues and much less histidine and proline. The P2 protein conjugated with peroxidase was bound by lymph node cells and infiltrates in rabbits sensitized with whole bovine root myelin. No binding was evident with the bovine central nervous system myelin basic protein. Chemically and immunologically, the P2 protein appears to be specific to peripheral nervous system myelin. The isolated P2 protein produced mild clinical symptoms of experimental allergic neuritis, but no histological evidence of disease. It was suggested that the P2 protein is an important antigen for experimental allergic neuritis, and that its antigenic determinants are likely to be conformation-dependent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1974.tb10756.x
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  • 4
    Electronic Resource
    Electronic Resource
    Laparoscopic appendectomy is an acceptable alternative for the treatment of perforated appendicitis (1998)
    Springer
    Surgical endoscopy and other interventional techniques 12 (1998), S. 940-943 
    Details
    ISSN: 1432-2218
    Keywords: Key words: Laparoscopic appendectomy—Open appendectomy—Endoscopic linear stapler—Perforated appendicitis—Abscess
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Ever since laparoscopy was first applied to the treatment of appendicitis, a controversy has existed as to whether the acknowledged benefits of a minimally invasive approach warrant its preference over the conventional treatment, which historically has had relatively low morbidity. The purpose of this study was to determine if laparoscopic appendectomy should be performed preferentially in cases where surgeons are not limited by technical constraints. Methods: A retrospective chart review was performed of 112 patients operated on for suspected appendicitis from June 1995 to July 1996. Forty-eight patients underwent laparoscopic appendectomy, and 64 had conventional open appendectomy. Laparoscopic appendectomy was performed using a three-trocar technique and the endoscopic stapler. Results: The histopathological diagnosis of appendicitis was confirmed in 82.6% of cases. Overall, laparoscopic appendectomy reduced length of hospital stay (1.54 versus 4.09 days; p 〈 0.0001) compared to conventional open appendectomy, with no significant difference in hospital cost ($6430 versus $6669; p= ns). Although the total OR time was longer in the laparoscopic group (75.8 versus 60.2 min; p 〈 0.0001), laparoscopy resulted in both a reduction in length of stay (2.17 versus 6.27 days; p 〈 0.0001) and hospital cost ($7506 versus $10,504; p 〈 0.02) for cases of perforated appendicitis. Conversion to open appendectomy was performed in 6% of patients, all of whom had perforated appendicitis. Conclusions: Our data suggest that most cases of acute appendicitis with suspected perforation could be managed laparoscopically. Laparoscopic appendectomy significantly reduces length of stay and hospital costs in patients with perforated appendicitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004649900752
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  • 5
    Electronic Resource
    Electronic Resource
    Choice of insufflating gas influences on wound metastasisrid=""id=""Presented at the annual meeting of the Society of American Gastrointestinal Endoscopic Surgeons (SAGES), San Antonio, Texas, USA, March 1999 (2000)
    Springer
    Surgical endoscopy and other interventional techniques 14 (2000), S. 1047 -1049 
    Details
    ISSN: 1432-2218
    Keywords: Key words: Colon cancer — Gases — Implantation — Laparoscopy — Pneumoperitoneum — Port site
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Laparoscopic cancer surgery is limited by concerns about port-site metastasis. No study has definitively addressed the behavior and growth of tumor cells after the use of specific laparoscopic gases. Methods: In athymic rats, 10,000 colon cancer cells were injected intraperitoneally. The rats received either no pneumoperitoneum (pneumo) or pneumo (8 mmHg, 10 min) with carbon dioxide (CO2), nitrous oxide (N2O), or air. Two full-thickness incisions were made and closed in the upper abdomen of each animal. After 4 weeks, implants were identified grossly at necropsy, and invasiveness was scored according to penetration through the layers of the abdominal wall. Results: Rats receiving pneumo had more frequent implants (p 〈 0.01) with deeper penetration (p 〈 0.001) than rats not receiving pneumo. Implants were more common after air pneumo than after CO2 (p 〈 0.05) or N2O (p= 0.07) pneumo, and were less penetrating after CO2 pneumo than after air (p 〈 0.001) or N2O (p 〈 0.05) pneumo. Conclusions: Carbon dioxide gas may limit the viability and invasiveness of free intraperitoneal tumor cells, as compared with air or N2O.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004640000219
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  • 6
    Electronic Resource
    Electronic Resource
    Laparoscopic Collis gastroplasty and Nissen fundoplication (1998)
    Springer
    Surgical endoscopy and other interventional techniques 12 (1998), S. 1055-1060 
    Details
    ISSN: 1432-2218
    Keywords: Key words: Hiatal hernia — Paraesophageal hernia — Gastroesophageal junction — Esophageal stricture — Collis gastroplasty — Laparoscopic Nissen fundoplication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The short esophagus increases the difficulty and limits the effectiveness of laparoscopic Nissen fundoplication. In our experience, ∼20–25% of esophagi judged by preoperative criteria to be foreshortened will, after dissection, be insufficiently long to allow 2 cm of esophagus to reside below the diaphragm without inferior distraction (i.e., tension free). Collis gastroplasty combined with Nissen fundoplication has become the standard approach for the creation of an intraabdominal neoesophagus and fundic wrap. Methods: After developing methods of performing totally laparoscopic stapled gastroplasty in the cadaver lab in 1994, we started applying the technique clinically in 1996. We performed 220 laparoscopic antireflux procedures between January 1996 and July 1997. Of these 220 patients, 26% were suspected to have esophageal foreshortening based on preoperative barium studies and/or endoscopy. Results: After hiatal dissection, nine patients, or 16% of those suspected to have esophageal foreshortening and 4% of the entire population, required the laparoscopic Collis-Nissen procedure. There was symptomatic improvement in all patients as assessed by patient-initiated symptom scores. Conclusions: The management of patients with esophageal foreshortening is a complex problem. We believe that our technique of laparoscopic Collis-Nissen provides an effective means of achieving intraabdominal placement of the fundic wrap while maintaining the benefits of a minimally invasive approach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004649900780
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