ISSN:
1573-5001
Keywords:
HPr
;
side-chain amide proton
;
solvent exchange rates
;
temperature coefficients
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values 〉 8. An NOE between a side-chain amide proton and a bound water molecule was also observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008296932751
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