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  • 1
    Electronic Resource
    Electronic Resource
    Relationship of the central ACTH-immunoreactive opiocortin system to the median eminence and the pituitary gland of the rat (1981)
    Springer
    Cell & tissue research 215 (1981), S. 333-340 
    Details
    ISSN: 1432-0878
    Keywords: ACTH-opiocortin system ; Immunocytochemistry ; Median eminence ; Neural lobe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By immunocytochemical methods, the present study describes ACTH-immunoreactive fibers in the pituitary stalk and neural lobe. This opiocortin-hypothalamo-neural lobe projection arises in a bed nucleus of perikarya in the basal hypothalamus, follows supraoptico-hypophyseal fibers in the zona interna of the median eminence, and distributes throughout the neural lobe. No ACTH-immunoreactive fibers project to the zona externa; some are present in the subependymal layer and at the lateral margins of the median eminence. Further studies must identify the role of these fibers in posterior lobe function. It remains also to be determined whether this system terminates upon primary pituitary portal capillaries and delivers opiocortin neuropeptides to the adenohypophysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239119
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunocytochemical distribution of LHRH neurons and processes in the rat: Hypothalamic and extrahypothalamic locations (1982)
    Springer
    Cell & tissue research 221 (1982), S. 493-504 
    Details
    ISSN: 1432-0878
    Keywords: Luteinizing hormone ; releasing hormone ; Immunocytochemistry ; Distribution ; Forebrain ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of luteinizing hormone-releasing hormone (LHRH)-immunoreactive perikarya and processes was examined, in the untreated rat, with the unlabeled antibody enzyme method of immunocytochemistry on thick 50 μm vibratome sections. LHRH neurons were primarily observed in the preoptico-anterior hypothalamic and septal areas. Projections from these cell bodies to the median eminence form three distinct pathways, one laterally along the course of the optic tracts, one medially through the periventricular stratum of the third ventricle, and one through the tractus infundibularis. In addition, some of these cell bodies project to the organum vasculosum of the lamina terminalis (OVLT) and the subfornical organ (SFO). LHRH immunoreactive neurons were also noted in the anterior olfactory regions; they project along the medial olfactory tract to the olfactory bulb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215698
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunoreactive adrenocorticotropin in rat brain: A neuroanatomical study using antiserum generated against synthetic ACTH1-39 (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 158 (1980), S. 533-548 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In this study an antiserum generated against synthetic ACTH1-39 is used to provide a neuroanatomical map of ACTH-immunoreactivity in rat brain. Groups of ACTH-immunoreactive cell bodies were located in the basal hypothalamus, extending from the retrochiasmatic region to the mammillary nuclei. The fiber distribution indicates a broad innervation of structures in the hypothalamus, which include the medial preoptic region; bed nucleus of the anterior commissure and stria terminalis; perifornical region; periventricular stratum; and paraventricular, dorsomedial, arcuate, and mammillary nuclei. Fibers from the hypothalamus reach the amygdaloid nuclei directly in two separate bundles. A large dorsal ACTH-immunoreactive bundle emerges from the anterior hypothalamic region, distributes to the periventricular thalamic nuclei, and continues more posteriorly to the midbrain periaquaductal gray. It is speculated that the cell bodies in the ventral tuberal region of the hypothalamus are responsible for the vast innervation of fibers to the forebrain, diencephalon, and brainstem.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001580413
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Dual immunostaining procedure demonstrating neurotransmitter and neuropeptide codistribution in the same brain section (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 175 (1986), S. 331-342 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A method for sequential immunostaining of two antigens in the same section and the application to neuronal elements in the CNS is described. This method employs the peroxidase-antiperoxidase (PAP) technique as a first sequence, followed by glucose oxidase-antiglucose oxidase (GAG) complex as the second sequence, yielding rich brown and vibrant blue reaction products, respectively. Application of the PAP/GAG immunoreagents as dual markers for various neuropeptide and neurotransmitter systems is described in detail and the procedure is outlined. Comparisons with other dual staining methods are discussed and the application to other systems is presented.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001750215
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    Paper (German National Licenses)
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