ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Biodegradable porous poly(L-lactic acid) (PLLA) scaffolds were prepared using gasfoaming method. The PLLA scaffolds with a hydrophobic surface were subjected to Ar plasmatreatment and in situ acrylic acid (AA) grafting to obtain hydrophilic PLLA scaffold (PLLA-PAA).Cell-adhesive RGD peptide was then immobilized onto the AA-grafted PLLA (PLLA-PAA-RGD).Once rabbit bone marrow-derived mesenchymal stem cells (BM-MSC) were isolated, MSCs wereseeded into PLLA control, PLLA-PAA, and PLLA-PAA-RGD scaffold and cultured for up to 4weeks in chondrogenic medium with the addition of 10 ng/ml transforming growth factor (TGF)-β1.Surface analysis of AA-grafted PLLA identified significant alterations of surface characteristics,including reduced contact angle and different atomic compositions. From WST-1 assay at 4 weeks,cells were found more proliferative in PLLA-PAA than the others. Upon the histological analysis ofSafranin O staining, chondrogenic differentiation of MSCs appeared to be progressed more activelyin PLLA-PAA. The effect of RGD immobilization on MSC differentiation was barely notable
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.297.pdf
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