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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 651 (1992), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Haemopoietic cells carry a variety of cell-surface molecules, some of which are known to have allotypic variation. In rats, the RT7 alloantigenic system has been well documented using alloantisera. We have produced the first mouse hybridomn cell line secreting an antibody. H1S41, which binds to leucocytes of rat strains carrying the RT7.2 but not the RT7.1 determinant. An lgG2b isotype switch variant (HIS4l.2b) of the original HIS41 (IgG1 isotype) was also made. HIS41 showed a clear and discrete binding in immunofluorescenl and histological experiments and has already been used in several studies on haemopoietic cell turnover and differentiation employing PVG rats congenic for RT7. The present study addresses the question of whether the RT7 gene products are members of the L-CA family, which has been a matter of controversy over the last decade. When using HIS41 for the analysis of tissue distribution and molecular weight of RT7 gene products, a strong similarity was evident with the data reported for the L-CA detected by MRC OX-1 and MRC OX-30. These two MoAb have been reported to bind to all members of the L-CA family. All haemopoietic cells, excluding erythrocytes and the more mature stages of erythropoiesis, stained with HIS41. The molecular weights of HIS41 binding molecules on thymocytes and peripheral T cells were comparable to the L-CA precipitated by MRC OX-1. Capping and sequential immunoprecipitation studies indicated that HIS41 and MRC OX-30- binding molecules were identical. MRC OX-1. however, appeared to bind only a subset of these molecules. Thus, our study confirms the identity of RT7.2 gene products and L-CA. lt also revealed a difference between MRC OX-1 and MRC OX-30 not noticed previously.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 196 (1979), S. 545-548 
    ISSN: 1432-0878
    Keywords: Fixed phagocytes ; Cellular defence ; Lymnaea stagnalis ; Freshwater snail
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Morphological and histochemical examination of the blood and connective tissue of the freshwater snail Lymnaea stagnalis injected with various types of foreign particulate materials has shown the existence of free as well as fixed phagocytic cells. The morphology of the fixed phagocytes is described, and the phagocytic system of the snail is compared with that of other molluscan species.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 231 (1983), S. 377-386 
    ISSN: 1432-0878
    Keywords: Chicken spleen ; Germinal centre ; Schweigger-Seidel sheath ; Follicular dendritic cells ; Immunohistoperoxidase technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By means of immunohistoperoxidase techniques and the use of HRP-anti-HRP complexes, follicular dendritic cells in chicken spleen can be characterized both at the light-microscopical and ultrastructural level. In contrast to findings in mammals follicular dendritic cells in chicken spleen exhibit evident acid-phosphatase activity and possess considerable numbers of primary lysosomes. After intravenous injection of immune complexes a transient immune complex-trapping occurs in the peripheral parts of the Schweigger-Seidel sheath. The immune complextrapping cells in the Schweigger-Seidel sheath and germinal centre show an identical enzyme histochemical pattern and only minor differences in ultrastructural characteristics. Shortly after intravenous injection of immune complexes and carbon particles these compounds show an identical distribution pattern; however, in the following days these distribution patterns become divergent.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 173 (1982), S. 279-284 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphology of the spleen of the Red-eared Slider, Chrysemys scripta elegans, has been described. The outer surface of the spleen is covered by a thin fibromuscular capsule, from which a number of trabeculae extend into the parenchyma. The parenchyma of the spleen shows a definite demarcation into a red and white pulp. The white pulp is composed of two lymphoid compartments: lymphoid tissue surrounds both central arterioles and ellipsoids, forming the periarteriolar and periellipsoidal lymphocyte sheath (PALS and PELS, respectively). The ellipsoids consist of a thick layer of reticular tissue, enclosing a capillary with cuboidal endothelium. In contrast with the PALS, reticular fibers are rarely found in the PELS. Even after severe stimulation with paratyphoid vaccine, germinal centers are not found in the spleen of C.s. elegans. The red pulp of the spleen is composed of a system of venous sinuses and cords.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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